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Yorodumi- PDB-1det: RIBONUCLEASE T1 CARBOXYMETHYLATED AT GLU 58 IN COMPLEX WITH 2'GMP -
+Open data
-Basic information
Entry | Database: PDB / ID: 1det | ||||||
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Title | RIBONUCLEASE T1 CARBOXYMETHYLATED AT GLU 58 IN COMPLEX WITH 2'GMP | ||||||
Components | RIBONUCLEASE T1 | ||||||
Keywords | HYDROLASE (ENDORIBONUCLEASE) / HYDROLASE / ENDORIBONUCLEASE / NUCLEASE / ENDONUCLEASE | ||||||
Function / homology | Function and homology information hyphal tip / ribonuclease T1 activity / ribonuclease T1 / cell septum / endonuclease activity / lyase activity / RNA binding Similarity search - Function | ||||||
Biological species | Aspergillus oryzae (mold) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.8 Å | ||||||
Authors | Ishikawa, K. / Suzuki, E. / Tanokura, M. / Takahashi, K. | ||||||
Citation | Journal: Biochemistry / Year: 1996 Title: Crystal structure of ribonuclease T1 carboxymethylated at Glu58 in complex with 2'-GMP. Authors: Ishikawa, K. / Suzuki, E. / Tanokura, M. / Takahashi, K. #1: Journal: Eur.J.Biochem. / Year: 1993 Title: Three-Dimensional Structure of the Ternary Complex between Ribonuclease T1, Guanosine 3',5'-Bisphosphate and Inorganic Phosphate at 0.19 Nm Resolution Authors: Lenz, A. / Choe, H.W. / Granzin, J. / Heinemann, U. / Saenger, W. #2: Journal: Acta Crystallogr.,Sect.B / Year: 1991 Title: X-Ray Analysis of Cubic Crystals of the Complex Formed between Ribonuclease T1 and Guanosine-3',5'-Bisphosphate Authors: Lenz, A. / Heinemann, U. / Maslowska, M. / Saenger, W. #3: Journal: Nature / Year: 1982 Title: Specific Protein-Nucleic Acid Recognition in Ribonuclease T1-2'-Guanylic Acid Complex: An X-Ray Study Authors: Heinemann, U. / Saenger, W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1det.cif.gz | 36.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1det.ent.gz | 24.4 KB | Display | PDB format |
PDBx/mmJSON format | 1det.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1det_validation.pdf.gz | 845.2 KB | Display | wwPDB validaton report |
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Full document | 1det_full_validation.pdf.gz | 847.7 KB | Display | |
Data in XML | 1det_validation.xml.gz | 5.5 KB | Display | |
Data in CIF | 1det_validation.cif.gz | 7.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/de/1det ftp://data.pdbj.org/pub/pdb/validation_reports/de/1det | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 11151.681 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: THE G-CARBOXYL GROUP OF GLU 58 IS CARBOXYMETHYLATED Source: (natural) Aspergillus oryzae (mold) / References: UniProt: P00651, EC: 3.1.27.3 | ||||
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#2: Chemical | #3: Chemical | ChemComp-2GP / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.61 Å3/Da / Density % sol: 52.9 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 4.5 / Details: pH 4.5 | ||||||||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 52.9 % | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 3.2 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 288 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: BL-6A / Wavelength: 1 |
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: May 16, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Highest resolution: 1.8 Å / Num. obs: 10459 / % possible obs: 95.9 % / Observed criterion σ(I): 1 / Redundancy: 6.5 % / Rmerge(I) obs: 0.057 |
Reflection shell | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 1.89 Å / % possible obs: 89.7 % |
-Processing
Software |
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Refinement | Resolution: 1.8→8 Å / σ(F): 1 /
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Displacement parameters | Biso mean: 32.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→8 Å
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Refine LS restraints |
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Refinement | *PLUS Rfactor obs: 0.194 / Rfactor Rwork: 0.194 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |