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- PDB-1d0w: SOLUTION STRUCTURE OF LACTAM-BRIDGED C-TERMINAL ANALOGUE-I OF NEU... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1d0w | ||||||
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Title | SOLUTION STRUCTURE OF LACTAM-BRIDGED C-TERMINAL ANALOGUE-I OF NEUROPEPTIDE Y | ||||||
![]() | C-TERMINAL ANALOGUE OF NEUROPEPTIDE Y, A POTENT Y2 RECEPTOR AGONIST | ||||||
![]() | NEUROPEPTIDE / LACTAM-BRIDGED / HELIX | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Yao, S. / Norton, R.S. | ||||||
![]() | ![]() Title: Stabilization of the helical structure of Y2-selective analogues of neuropeptide Y by lactam bridges. Authors: Yao, S. / Smith-White, M.A. / Potter, E.K. / Norton, R.S. #1: ![]() Title: Helical structure and self-association in a 13-residue neuropeptide Y Y2 receptor agonist: relationship to biological activity Authors: Barnham, K.J. / Catalfamo, F. / Pallaghy, P.K. / Howlett, G.J. / Norton, R.S. #2: ![]() Title: Solution structure of human neuropeptide Y Authors: Monks, S.A. / Karagianis, G. / Howlett, G.J. / Norton, R.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 93 KB | Display | ![]() |
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PDB format | ![]() | 71.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 352.4 KB | Display | ![]() |
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Full document | ![]() | 456.5 KB | Display | |
Data in XML | ![]() | 7.1 KB | Display | |
Data in CIF | ![]() | 10.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 1792.054 Da / Num. of mol.: 1 / Fragment: C-TERMINAL ANALOGUE Mutation: L24A, I28K, I31L, T32E, A LACTAM-BRIDGE WAS INTRODUCED BETWEEN POSITIONS (28) AND (32) Source method: obtained synthetically / Details: Chemically synthesized. |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: THIS STRUCTURE WAS DETERMINED USING STANDARD 2D HOMONUCLEAR TECHNIQUES. |
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Sample preparation
Details | Contents: 3.5 MG IN 0.6 ML H2O CONTAINING 30% TFE-D3 (BY VOLUME) |
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Sample conditions | pH: 5.0 / Pressure: 1 atm / Temperature: 308 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: THE STRUCTURE WAS CALCULATED USING A TOTAL OF 124 UPPER BOUND DISTANCE RESTRAINTS AND 8 BACKBONE DIHEDRAL ANGLE CONSTRAINTS | ||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |