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Yorodumi- PDB-1cx4: CRYSTAL STRUCTURE OF A DELETION MUTANT OF THE TYPE II BETA REGULA... -
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-Basic information
Entry | Database: PDB / ID: 1cx4 | ||||||
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Title | CRYSTAL STRUCTURE OF A DELETION MUTANT OF THE TYPE II BETA REGULATORY SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE | ||||||
Components | CAMP-DEPENDENT PROTEIN KINASE REGULATORY SUBUNIT TYPE II BETACAMP-dependent pathway | ||||||
Keywords | SIGNALING PROTEIN / BETA BARREL / CAMP-DEPENDENT PROTEIN KINASE / CAMP-BINDING / REGULATORY SUBUNIT | ||||||
Function / homology | Function and homology information GPER1 signaling / PKA activation in glucagon signalling / CREB1 phosphorylation through the activation of Adenylate Cyclase / DARPP-32 events / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 ...GPER1 signaling / PKA activation in glucagon signalling / CREB1 phosphorylation through the activation of Adenylate Cyclase / DARPP-32 events / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / Factors involved in megakaryocyte development and platelet production / Regulation of PLK1 Activity at G2/M Transition / Hedgehog 'off' state / PKA activation / response to antipsychotic drug / cAMP-dependent protein kinase regulator activity / Vasopressin regulates renal water homeostasis via Aquaporins / cAMP-dependent protein kinase inhibitor activity / ciliary base / cAMP-dependent protein kinase complex / protein kinase A catalytic subunit binding / cAMP binding / fatty acid metabolic process / dendritic shaft / learning / regulation of protein phosphorylation / modulation of chemical synaptic transmission / postsynapse / dendritic spine / protein domain specific binding / centrosome / dendrite / neuronal cell body / glutamatergic synapse / ubiquitin protein ligase binding / protein kinase binding / perinuclear region of cytoplasm / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.45 Å | ||||||
Authors | Diller, T.C. / Xuong, N.H. / Taylor, S.S. | ||||||
Citation | Journal: Structure / Year: 2001 Title: Molecular basis for regulatory subunit diversity in cAMP-dependent protein kinase: crystal structure of the type II beta regulatory subunit. Authors: Diller, T.C. / Madhusudan / Xuong, N.H. / Taylor, S.S. #1: Journal: Protein Expr.Purif. / Year: 2000 Title: Type II beta regulatory subunit of cAMP-dependent protein kinase: purification strategies to optimize crystallization. Authors: Diller, T.C. / Xuong, N.H. / Taylor, S.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1cx4.cif.gz | 73.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1cx4.ent.gz | 54.3 KB | Display | PDB format |
PDBx/mmJSON format | 1cx4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cx/1cx4 ftp://data.pdbj.org/pub/pdb/validation_reports/cx/1cx4 | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 34157.719 Da / Num. of mol.: 1 / Fragment: CAMP BINDING DOMAINS Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Plasmid: INVITROGEN PRSET B / Production host: Escherichia coli (E. coli) / References: UniProt: P12369 | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.38 Å3/Da / Density % sol: 70.8 % Description: PROTEIN RESIDUES PRECEDING ARG 130 AND POSTERIOR TO VAL 413 IN THE AMINO ACID SEQUENCE ARE NOT INCLUDED IN THIS MODEL. ADDITIONALLY, LOOP REGION CONSISTING OF RESIDUES 326 LYS THROUGH ...Description: PROTEIN RESIDUES PRECEDING ARG 130 AND POSTERIOR TO VAL 413 IN THE AMINO ACID SEQUENCE ARE NOT INCLUDED IN THIS MODEL. ADDITIONALLY, LOOP REGION CONSISTING OF RESIDUES 326 LYS THROUGH 333 GLU WERE NOT TRACED IN ELECTRON DENSITY MAPS: NO PEPTIDE BOND EXISTS BETWEEN ARG 325 AND ASN 334 IN THIS STRUCTURAL MODEL. | |||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.75 / Details: pH 7.75 | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging dropDetails: Diller, T.C., (2000) Protein Expression Purif., 20, 357. | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 300.3 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL7-1 / Wavelength: 1.08 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jan 6, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 2.45→25 Å / Num. obs: 21965 / % possible obs: 98.7 % / Observed criterion σ(I): 0 / Redundancy: 4.182 % / Biso Wilson estimate: 36.4 Å2 / Rmerge(I) obs: 0.052 / Net I/σ(I): 14.3 |
Reflection shell | Resolution: 2.45→2.56 Å / Redundancy: 4 % / Rmerge(I) obs: 0.325 / Mean I/σ(I) obs: 3.94 / % possible all: 97.9 |
Reflection shell | *PLUS % possible obs: 97.9 % |
-Processing
Software |
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Refinement | Resolution: 2.45→23 Å / Rfactor Rfree error: 0.004 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: ENGH & HUBER Details: INSUFFICIENT ELECTRON DENSITY EXISTED IN MAPS TO INCLUDE RESIDUE RANGES 112- 128, 326-333 AND 413-416.
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 49.37 Å2 / ksol: 0.33 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 43.6 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.45→23 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.45→2.56 Å / Rfactor Rfree error: 0.02 / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.5 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 23 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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