[English] 日本語
Yorodumi- PDB-1ceu: NMR STRUCTURE OF THE (1-51) N-TERMINAL DOMAIN OF THE HIV-1 REGULA... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ceu | ||||||
---|---|---|---|---|---|---|---|
Title | NMR STRUCTURE OF THE (1-51) N-TERMINAL DOMAIN OF THE HIV-1 REGULATORY PROTEIN | ||||||
Components | PROTEIN (HIV-1 REGULATORY PROTEIN N-TERMINAL DOMAIN VPR) | ||||||
Keywords | VIRAL PROTEIN / REGULATORY PROTEIN / HELICAL DOMAIN / AMPHIPATICITY | ||||||
Function / homology | Function and homology information symbiont-mediated arrest of host cell cycle during G2/M transition / monoatomic ion transmembrane transport / virion component / protein homooligomerization / viral penetration into host nucleus / host cell / host extracellular space / symbiont entry into host cell / cell cycle / DNA-templated transcription ...symbiont-mediated arrest of host cell cycle during G2/M transition / monoatomic ion transmembrane transport / virion component / protein homooligomerization / viral penetration into host nucleus / host cell / host extracellular space / symbiont entry into host cell / cell cycle / DNA-templated transcription / host cell nucleus / regulation of DNA-templated transcription Similarity search - Function | ||||||
Method | SOLUTION NMR / DYNAMICAL SIMULATED ANNEALING, MOLECULAR DYNAMICS, ENERGY MINIMIZATION | ||||||
Authors | Wecker, K. / Roques, B.P. | ||||||
Citation | Journal: Eur.J.Biochem. / Year: 1999 Title: NMR structure of the (1-51) N-terminal domain of the HIV-1 regulatory protein Vpr. Authors: Wecker, K. / Roques, B.P. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1ceu.cif.gz | 26.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1ceu.ent.gz | 16.9 KB | Display | PDB format |
PDBx/mmJSON format | 1ceu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1ceu_validation.pdf.gz | 244.3 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1ceu_full_validation.pdf.gz | 244 KB | Display | |
Data in XML | 1ceu_validation.xml.gz | 2.3 KB | Display | |
Data in CIF | 1ceu_validation.cif.gz | 2.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ce/1ceu ftp://data.pdbj.org/pub/pdb/validation_reports/ce/1ceu | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 6172.783 Da / Num. of mol.: 1 / Fragment: 1-51 / Source method: obtained synthetically Details: THIS PROTEIN WAS CHEMICALLY SYNTHESIZED BY SOLID PHASE SYNTHESIS. THE SEQUENCE OF THIS PROTEIN IS FOUND IN THE VPR GENE, HIV-1 LAI ISOLATE VARIANT OF V6750. References: UniProt: Q73369 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||||||
NMR details | Text: THE STRUCTURE WAS DETERMINED USING 1H-NMR SPECTROSCOPY ON THE N-TERMINAL DOMAIN (1-51)VPR. |
-Sample preparation
Sample conditions | pH: 3.4 / Pressure: 1 atm / Temperature: 323 K |
---|---|
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker AMX600 / Manufacturer: Bruker / Model: AMX600 / Field strength: 600 MHz |
---|
-Processing
NMR software |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: DYNAMICAL SIMULATED ANNEALING, MOLECULAR DYNAMICS, ENERGY MINIMIZATION Software ordinal: 1 Details: FIFTY STRUCTURES WERE GENERATED BY SIMULATED ANNEALING AND ENERGY MINIMIZATION. THE BEST STRUCTURE SELECTED WITH RESPECT TO RESTRAINT VIOLATIONS AND TOTAL ENERGY IS PRESENTED IN THIS ENTRY. | ||||||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATIONS, LOWEST TOTAL ENERGY Conformers calculated total number: 16 / Conformers submitted total number: 1 |