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Yorodumi- PDB-1c1g: CRYSTAL STRUCTURE OF TROPOMYOSIN AT 7 ANGSTROMS RESOLUTION IN THE... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1c1g | ||||||
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Title | CRYSTAL STRUCTURE OF TROPOMYOSIN AT 7 ANGSTROMS RESOLUTION IN THE SPERMINE-INDUCED CRYSTAL FORM | ||||||
Components | TROPOMYOSIN | ||||||
Keywords | CONTRACTILE PROTEIN / TROPOMYOSIN COILED-COIL ALPHA-HELICAL | ||||||
Function / homology | Function and homology information cardiac muscle contraction / actin filament / actin filament organization / actin filament binding / actin cytoskeleton / protein heterodimerization activity / protein homodimerization activity / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | Sus scrofa (pig) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SIR / Resolution: 7 Å | ||||||
Authors | Whitby, F.G. / Phillips Jr., G.N. | ||||||
Citation | Journal: Proteins / Year: 2000 Title: Crystal structure of tropomyosin at 7 Angstroms resolution. Authors: Whitby, F.G. / Phillips Jr., G.N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1c1g.cif.gz | 204 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1c1g.ent.gz | 159.3 KB | Display | PDB format |
PDBx/mmJSON format | 1c1g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1c1g_validation.pdf.gz | 418.4 KB | Display | wwPDB validaton report |
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Full document | 1c1g_full_validation.pdf.gz | 479.8 KB | Display | |
Data in XML | 1c1g_validation.xml.gz | 29.9 KB | Display | |
Data in CIF | 1c1g_validation.cif.gz | 43 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/c1/1c1g ftp://data.pdbj.org/pub/pdb/validation_reports/c1/1c1g | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 32784.637 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / Tissue: CARDIAC MUSCLE / References: UniProt: P42639 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.7 Å3/Da / Density % sol: 68 % Description: Thimerosal was pre-reacted with purified tropomyosin prior growing crystals (spermine-induced crystal form). One mercury reacts with cyteine-190 of each chain of the molecule. Thus four ...Description: Thimerosal was pre-reacted with purified tropomyosin prior growing crystals (spermine-induced crystal form). One mercury reacts with cyteine-190 of each chain of the molecule. Thus four mercury atoms are present in the asymmetric unit. Two mercury atoms are located adjecent to each other on neighboring chains on the molecule, and for heavy-atom phasing purposes, these pairs were considered as a single point scatterer (due to 7 angstrom resolution limit). SIR phases determined from this derivative showed clear molecular packing consistent with the previously determined 9 angstrom structure in the same crystal form which was determined by molecular replacement using a highly simplified model (Whitby, et al. JMB 1992;227:441-452) | ||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: liquid diffusion / pH: 7.4 Details: SPERMINE, pH 7.4, LIQUID DIFFUSION, temperature 18K | ||||||||||||||||||||
Crystal grow | *PLUS Temperature: 15-17.5 ℃ | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 21 K | |||||||||
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X8C / Wavelength: 1.000, 1.54 | |||||||||
Detector | Detector: CCD | |||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 7→100 Å / Num. obs: 3117 / % possible obs: 96.38 % / Observed criterion σ(I): 0 / Redundancy: 3.4 % |
-Processing
Software |
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Refinement | Method to determine structure: SIR / Resolution: 7→100 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 7→100 Å
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LS refinement shell | Resolution: 7→7.25 Å / Total num. of bins used: 10
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