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Open data
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Basic information
Entry | Database: PDB / ID: 1br9 | ||||||
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Title | HUMAN TISSUE INHIBITOR OF METALLOPROTEINASE-2 | ||||||
![]() | METALLOPROTEINASE-2 INHIBITOR | ||||||
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Function / homology | ![]() negative regulation of metallopeptidase activity / ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tuuttila, A. / Morgunova, E. / Bergmann, U. / Lindqvist, Y. / Tryggvason, K. / Schneider, G. | ||||||
![]() | ![]() Title: Three-dimensional structure of human tissue inhibitor of metalloproteinases-2 at 2.1 A resolution. Authors: Tuuttila, A. / Morgunova, E. / Bergmann, U. / Lindqvist, Y. / Maskos, K. / Fernandez-Catalan, C. / Bode, W. / Tryggvason, K. / Schneider, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 48 KB | Display | ![]() |
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PDB format | ![]() | 34.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 21783.039 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 38.74 % | ||||||||||||||||||||||||||||||
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Crystal grow![]() | pH: 8 / Details: pH 8.0 | ||||||||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.1→15 Å / Num. obs: 10532 / % possible obs: 98.8 % / Redundancy: 10 % / Rmerge(I) obs: 0.083 / Rsym value: 0.083 / Net I/σ(I): 15.4 |
Reflection shell | Resolution: 2.1→2.2 Å / Rmerge(I) obs: 0.284 / Mean I/σ(I) obs: 4.6 / Rsym value: 0.284 / % possible all: 98.5 |
Reflection | *PLUS Num. measured all: 100589 |
Reflection shell | *PLUS % possible obs: 98.5 % |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: BOVINE TISSUE INHIBITOR, FERNANDEZ-CATALAN ET AL., 1998, EMBO J., IN PRESS Resolution: 2.1→15 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Details: INITIAL REFINEMENT WITH X-PLOR, THEN REFMAC
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Displacement parameters | Biso mean: 34 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→15 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.238 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |