+
Open data
-
Basic information
Entry | Database: PDB / ID: 1br9 | ||||||
---|---|---|---|---|---|---|---|
Title | HUMAN TISSUE INHIBITOR OF METALLOPROTEINASE-2 | ||||||
![]() | METALLOPROTEINASE-2 INHIBITOR | ||||||
![]() | PROTEINASE INHIBITOR | ||||||
Function / homology | ![]() negative regulation of metallopeptidase activity / negative regulation of membrane protein ectodomain proteolysis / peptidase inhibitor activity / metalloendopeptidase inhibitor activity / molecular function inhibitor activity / Activation of Matrix Metalloproteinases / extracellular matrix / response to hormone / response to cytokine / specific granule lumen ...negative regulation of metallopeptidase activity / negative regulation of membrane protein ectodomain proteolysis / peptidase inhibitor activity / metalloendopeptidase inhibitor activity / molecular function inhibitor activity / Activation of Matrix Metalloproteinases / extracellular matrix / response to hormone / response to cytokine / specific granule lumen / tertiary granule lumen / protease binding / collagen-containing extracellular matrix / ficolin-1-rich granule lumen / Neutrophil degranulation / extracellular space / zinc ion binding / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tuuttila, A. / Morgunova, E. / Bergmann, U. / Lindqvist, Y. / Tryggvason, K. / Schneider, G. | ||||||
![]() | ![]() Title: Three-dimensional structure of human tissue inhibitor of metalloproteinases-2 at 2.1 A resolution. Authors: Tuuttila, A. / Morgunova, E. / Bergmann, U. / Lindqvist, Y. / Maskos, K. / Fernandez-Catalan, C. / Bode, W. / Tryggvason, K. / Schneider, G. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 48 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 34.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 21783.039 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
---|---|
#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 38.74 % | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | pH: 8 / Details: pH 8.0 | ||||||||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9095 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→15 Å / Num. obs: 10532 / % possible obs: 98.8 % / Redundancy: 10 % / Rmerge(I) obs: 0.083 / Rsym value: 0.083 / Net I/σ(I): 15.4 |
Reflection shell | Resolution: 2.1→2.2 Å / Rmerge(I) obs: 0.284 / Mean I/σ(I) obs: 4.6 / Rsym value: 0.284 / % possible all: 98.5 |
Reflection | *PLUS Num. measured all: 100589 |
Reflection shell | *PLUS % possible obs: 98.5 % |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: BOVINE TISSUE INHIBITOR, FERNANDEZ-CATALAN ET AL., 1998, EMBO J., IN PRESS Resolution: 2.1→15 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Details: INITIAL REFINEMENT WITH X-PLOR, THEN REFMAC
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 34 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→15 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.238 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |