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Open data
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Basic information
Entry | Database: PDB / ID: 1bhm | ||||||
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Title | RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA | ||||||
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![]() | HYDROLASE/DNA / HYDROLASE / COMPLEX (ENDONUCLEASE-DNA) / NUCLEASE / HYDROLASE-DNA COMPLEX | ||||||
Function / homology | ![]() type II site-specific deoxyribonuclease / type II site-specific deoxyribonuclease activity / DNA restriction-modification system / magnesium ion binding / DNA binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Aggarwal, A.K. / Newman, M. | ||||||
![]() | ![]() Title: Structure of Bam HI endonuclease bound to DNA: partial folding and unfolding on DNA binding. Authors: Newman, M. / Strzelecka, T. / Dorner, L.F. / Schildkraut, I. / Aggarwal, A.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 108.6 KB | Display | ![]() |
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PDB format | ![]() | 81.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 385.8 KB | Display | ![]() |
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Full document | ![]() | 410.6 KB | Display | |
Data in XML | ![]() | 12.7 KB | Display | |
Data in CIF | ![]() | 19.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT CONTAINS TWO BAMHI MONOMERS (CHAINS A AND B) BOUND TO A PALINDROMIC 12 BASE PAIR DNA COMPLEX. |
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Components
#1: DNA chain | Mass: 3661.416 Da / Num. of mol.: 2 / Source method: obtained synthetically #2: Protein | Mass: 24602.297 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() References: UniProt: P23940, type II site-specific deoxyribonuclease #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 54.41 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop / Details: VAPOR DIFFUSION, HANGING DROP / Temp details: ROOM TEMPERATURE | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Details: Strzelecka, T., (1994) J. Mol. Biol, 239, 430. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.2→30 Å / Num. obs: 30078 / % possible obs: 94.2 % |
Reflection | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 30 Å / % possible obs: 94.2 % / Num. measured all: 103881 / Rmerge(I) obs: 0.118 |
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Processing
Software | Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||
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Refinement | Resolution: 2.2→10 Å / σ(F): 2 Details: THE 3' ADENINE RESIDUE ON ONE OF THE DNA STRANDS IS DISORDERED.
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Refinement step | Cycle: LAST / Resolution: 2.2→10 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 10 Å / σ(F): 2 | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: t_angle_deg / Dev ideal: 2.8 |