+Open data
-Basic information
Entry | Database: PDB / ID: 1b8t | ||||||
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Title | SOLUTION STRUCTURE OF THE CHICKEN CRP1 | ||||||
Components | PROTEIN (CRP1) | ||||||
Keywords | CONTRACTILE / LIM DOMAIN / CRP / MUSCLE DIFFERENTIATION | ||||||
Function / homology | Function and homology information muscle tissue development / actinin binding / structural constituent of muscle / sarcomere organization / cell leading edge / alpha-actinin binding / stress fiber / phosphoprotein binding / Z disc / focal adhesion ...muscle tissue development / actinin binding / structural constituent of muscle / sarcomere organization / cell leading edge / alpha-actinin binding / stress fiber / phosphoprotein binding / Z disc / focal adhesion / nucleus / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Gallus gallus (chicken) | ||||||
Method | SOLUTION NMR / DYANA | ||||||
Authors | Yao, X. / Perez-Alvarado, G.C. / Louis, H.A. / Pomies, P. / Hatt, C. / Summers, M.F. / Beckerle, M.C. | ||||||
Citation | Journal: Biochemistry / Year: 1999 Title: Solution structure of the chicken cysteine-rich protein, CRP1, a double-LIM protein implicated in muscle differentiation. Authors: Yao, X. / Perez-Alvarado, G.C. / Louis, H.A. / Pomies, P. / Hatt, C. / Summers, M.F. / Beckerle, M.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1b8t.cif.gz | 2 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1b8t.ent.gz | 1.7 MB | Display | PDB format |
PDBx/mmJSON format | 1b8t.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1b8t_validation.pdf.gz | 347.1 KB | Display | wwPDB validaton report |
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Full document | 1b8t_full_validation.pdf.gz | 722.8 KB | Display | |
Data in XML | 1b8t_validation.xml.gz | 165.7 KB | Display | |
Data in CIF | 1b8t_validation.cif.gz | 206.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b8/1b8t ftp://data.pdbj.org/pub/pdb/validation_reports/b8/1b8t | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 20348.316 Da / Num. of mol.: 1 / Fragment: LIM-DOMAIN PROTEIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Gallus gallus (chicken) / Tissue: MUSCLE / Plasmid: PET5 / Cell line (production host): BL21(DE3) / Production host: Escherichia coli (E. coli) / References: UniProt: P67966 |
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#2: Chemical | ChemComp-ZN / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING HOMONUCLEAR DATA ON THE TWO INDIVIDUAL DOMAINS OF CRP1 AND N15-EDITED NOESY AND TOCSY DATA ON N15-LABELED CRP1. |
-Sample preparation
Sample conditions | pH: 7.2 / Temperature: 298 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: GE OMEGA PSG / Manufacturer: GE / Model: OMEGA PSG / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: DYANA / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE | |||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 37 / Conformers submitted total number: 37 |