[English] 日本語
Yorodumi- PDB-1b5n: NMR STRUCTURE OF PSP1, PLASMATOCYTE-SPREADING PEPTIDE FROM PSEUDO... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1b5n | ||||||
---|---|---|---|---|---|---|---|
Title | NMR STRUCTURE OF PSP1, PLASMATOCYTE-SPREADING PEPTIDE FROM PSEUDOPLUSIA INCLUDENS | ||||||
Components | PROTEIN (PLASMATOCYTE-SPREADING PEPTIDE) | ||||||
Keywords | SIGNALING PROTEIN / INSECT CYTOKINE / PLASMATOCYTE-SPREADING / EGF-LIKE | ||||||
Function / homology | Paralytic/GBP/PSP peptide / Paralytic/GBP/PSP peptide / cytokine activity / extracellular space / Plasmatocyte-spreading peptide Function and homology information | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Volkman, B.F. / Clark, K.D. / Anderson, M.E. / Pech, L.L. / Markley, J.L. / Strand, M.R. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 1999 Title: Structure of the insect cytokine peptide plasmatocyte-spreading peptide 1 from Pseudoplusia includens. Authors: Volkman, B.F. / Anderson, M.E. / Clark, K.D. / Hayakawa, Y. / Strand, M.R. / Markley, J.L. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1b5n.cif.gz | 14.8 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1b5n.ent.gz | 8.1 KB | Display | PDB format |
PDBx/mmJSON format | 1b5n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1b5n_validation.pdf.gz | 242.3 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1b5n_full_validation.pdf.gz | 242.1 KB | Display | |
Data in XML | 1b5n_validation.xml.gz | 1.7 KB | Display | |
Data in CIF | 1b5n_validation.cif.gz | 1.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b5/1b5n ftp://data.pdbj.org/pub/pdb/validation_reports/b5/1b5n | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein/peptide | Mass: 2511.835 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: THE PEPTIDE WAS CHEMICALLY SYNTHESIZED BY SOLID-STATE PEPTIDE SYNTHESIS WITH T-BOC METHOD. THE SEQUENCE OF THIS PEPTIDE IS NATURALLY FOUND IN THE HEMOLYMPH TISSUE OF PSEUDOPLUSIA INCLUDENS (SOYBEAN LOOPER MOTH). References: UniProt: O61704 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||
NMR details | Text: MEAN STRUCTURE. BOTH: QUADRATURE IN INDIRECT DIM. WAS OBTAINED WITH STATES- TPPI. WATER SUPPRESION WAS ACHIEVED WITH A WATERGATE SEQ. WITH A 3-9-19 SELECTIVE INVERSION |
-Sample preparation
Sample conditions | Ionic strength: 50 mM PO4 BUFFER / pH: 6 / Pressure: 1 atm / Temperature: 283 K |
---|---|
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: torsion angle dynamics / Software ordinal: 1 Details: THE FILES IN THE DATABASE SHOULD BE SUFFICIENT TO RECONSTRUCT THE STRUCTURE. ALSO REFER TO THE REF. ABOVE AND THE PSP1 PAPER. | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST TARGET FUNCTION / Conformers submitted total number: 1 |