RESTRAINT ENERGY VIOLATION WITHIN 30% OF THE CONFORMER OF LOWER ENERGY VIOLATION. 28 CONFORMERS SATISFIED THE CRITERIA, ONLY 10 ARE SHOWN.
Representative
-
Components
#1: Protein
SACY
Mass: 6365.466 Da / Num. of mol.: 2 / Fragment: RNA BINDING DOMAIN, RESIDUES 1 - 55 Source method: isolated from a genetically manipulated source Details: N TERMINAL REGION OF THE SACY PROTEIN. THE STRUCTURE IS A SYMMETRIC DIMER IN SOLUTION Source: (gene. exp.) Bacillus subtilis (bacteria) / Cell line: BL21 / Plasmid: PGEX-2T / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P15401
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
NOESY
1
2
1
TOCSY
1
3
1
COSY
1
4
1
HSQC
-
Sample preparation
Sample conditions
pH: 6 / Temperature: 297 K
Crystal grow
*PLUS
Method: other / Details: NMR
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NMR measurement
NMR spectrometer
Type: Bruker AMX600 / Manufacturer: Bruker / Model: AMX600 / Field strength: 600 MHz
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Processing
Software
Name
Version
Classification
X-PLOR
3.1
modelbuilding
X-PLOR
3.1
refinement
X-PLOR
3.1
phasing
NMR software
Name
Version
Developer
Classification
X-PLOR
3.1
BRUNGER
refinement
X-PLOR
3.1
structuresolution
Refinement
Method: simulated annealing / Software ordinal: 1 Details: THE MONOMERIC UNIT WAS MODELLED FIRST USING SAFE NOE WITH THE DG-SA PROTOCOL PROVIDED WITH X-PLOR. THE DIMER WAS THEN GENERATED BY ADDING EXTRA INTERMOLECULAR CONSTRAINTS PLUS A LIST OF ...Details: THE MONOMERIC UNIT WAS MODELLED FIRST USING SAFE NOE WITH THE DG-SA PROTOCOL PROVIDED WITH X-PLOR. THE DIMER WAS THEN GENERATED BY ADDING EXTRA INTERMOLECULAR CONSTRAINTS PLUS A LIST OF AMBIGUOUS (INTER OR INTRA MOLECULAR) RESTRAINTS USING THE PROTOCOL DESCRIBED IN NILGES (1993), PROTEINS 17, 297-309.
NMR ensemble
Conformer selection criteria: RESTRAINT ENERGY VIOLATION WITHIN 30% OF THE CONFORMER OF LOWER ENERGY VIOLATION. 28 CONFORMERS SATISFIED THE CRITERIA, ONLY 10 ARE SHOWN. Conformers calculated total number: 40 / Conformers submitted total number: 10
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