|Entry||Database: PDB / ID: 1atp|
|Title||2.2 angstrom refined crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MNATP and a peptide inhibitor|
|Function / homology||DARPP-32 events / AGC-kinase, C-terminal / Interleukin-3, Interleukin-5 and GM-CSF signaling / Protein kinase-like domain superfamily / VEGFA-VEGFR2 Pathway / Serine/threonine-protein kinase, active site / cAMP-dependent protein kinase inhibitor / Vasopressin regulates renal water homeostasis via Aquaporins / Protein kinase domain / Protein kinase domain ...DARPP-32 events / AGC-kinase, C-terminal / Interleukin-3, Interleukin-5 and GM-CSF signaling / Protein kinase-like domain superfamily / VEGFA-VEGFR2 Pathway / Serine/threonine-protein kinase, active site / cAMP-dependent protein kinase inhibitor / Vasopressin regulates renal water homeostasis via Aquaporins / Protein kinase domain / Protein kinase domain / Regulation of insulin secretion / Rap1 signalling / Loss of Nlp from mitotic centrosomes / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / Recruitment of NuMA to mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Regulation of PLK1 Activity at G2/M Transition / cAMP-dependent protein kinase catalytic subunit alpha / Protein kinase, ATP binding site / Ion homeostasis / AURKA Activation by TPX2 / PKA activation / AGC-kinase C-terminal domain profile. / Protein kinase domain profile. / HDL assembly / Serine/Threonine protein kinases active-site signature. / GLI3 is processed to GLI3R by the proteasome / Protein kinases ATP-binding region signature. / Factors involved in megakaryocyte development and platelet production / RET signaling / MAPK6/MAPK4 signaling / CD209 (DC-SIGN) signaling / Anchoring of the basal body to the plasma membrane / Hedgehog 'off' state / cAMP-dependent protein kinase inhibitor / PKA activation in glucagon signalling / calcium-dependent protein kinase activity / negative regulation of meiotic cell cycle / regulation of cellular respiration / sperm midpiece / nucleotide-activated protein kinase complex / negative regulation of protein import into nucleus / negative regulation of cAMP-dependent protein kinase activity / cAMP-dependent protein kinase inhibitor activity / negative regulation of catalytic activity / cAMP-dependent protein kinase / regulation of protein processing / negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning / cellular response to parathyroid hormone stimulus / cAMP-dependent protein kinase activity / regulation of bicellular tight junction assembly / cAMP-dependent protein kinase complex / ciliary base / protein kinase A regulatory subunit binding / sperm capacitation / protein kinase A signaling / axoneme / sperm flagellum / mesoderm formation / regulation of osteoblast differentiation / plasma membrane raft / intercellular bridge / Rab GTPase binding / protein kinase A catalytic subunit binding / regulation of synaptic transmission, glutamatergic / acrosomal vesicle / positive regulation of cell cycle arrest / regulation of proteasomal protein catabolic process / negative regulation of protein kinase activity / cellular response to glucose stimulus / neural tube closure / protein serine/threonine/tyrosine kinase activity / positive regulation of protein export from nucleus / modulation of chemical synaptic transmission / neuromuscular junction / mRNA processing / regulation of G2/M transition of mitotic cell cycle / cellular response to heat / peptidyl-threonine phosphorylation / manganese ion binding / dendritic spine / peptidyl-serine phosphorylation / protein-containing complex binding / nuclear speck / neuron projection / protein kinase activity / centrosome / protein autophosphorylation / protein domain specific binding / ubiquitin protein ligase binding / protein serine/threonine kinase activity / protein phosphorylation / positive regulation of cell population proliferation / protein kinase binding / Golgi apparatus / negative regulation of transcription by RNA polymerase II / perinuclear region of cytoplasm / magnesium ion binding / mitochondrion / protein-containing complex|
Function and homology information
|Specimen source||Mus musculus (house mouse)|
|Method||X-RAY DIFFRACTION / 2.2 Å resolution|
|Authors||Zheng, J. / Trafny, E.A. / Knighton, D.R. / Xuong, N.-H. / Taylor, S.S. / Teneyck, L.F. / Sowadski, J.M.|
Journal: Acta Crystallogr.,Sect.D / Year: 1993
Title: 2.2 A refined crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MnATP and a peptide inhibitor.
Authors: Zheng, J. / Trafny, E.A. / Knighton, D.R. / Xuong, N.H. / Taylor, S.S. / Ten Eyck, L.F. / Sowadski, J.M.
#1: Journal: To be Published
Title: 2.0 Angstrom Refined Crystal Structure of the Catalytic Subunit of Camp-Dependent Protein Kinase Complexed with a Peptide Inhibitor and Detergent
Authors: Knighton, D.R. / Bell, S.M. / Zheng, J. / Teneyck, L.F. / Xuong, N.-H. / Taylor, S.S. / Sowadski, J.M.
#2: Journal: Biochemistry / Year: 1993
Title: Crystal Structure of the Catalytic Subunit of cAMP-Dependent Protein Kinase Complexed with Mg/ATP and Peptide Inhibitor
Authors: Zheng, J. / Knighton, D.R. / Teneyck, L.F. / Karlsson, R. / Xuong, N.-H. / Taylor, S.S. / Sowadski, J.M.
#3: Journal: Science / Year: 1991
Title: Crystal Structure of the Catalytic Subunit of Cyclic Adenosine Monophosphate-Dependent Protein Kinase
Authors: Knighton, D.R. / Zheng, J. / Teneyck, L.F. / Xuong, V.A. / Ashford, N.-H. / Taylor, S.S. / Sowadski, J.M.
#4: Journal: Science / Year: 1991
Title: Structure of a Peptide Inhibitor Bound to the Catalytic Subunit of Cyclic Adenosine Monophosphate-Dependent Protein Kinase
Authors: Knighton, D.R. / Zheng, J. / Teneyck, L.F. / Xuong, N.-H. / Taylor, S.S. / Sowadski, J.M.
SummaryFull reportAbout validation report
|Date||Deposition: Jan 8, 1993 / Release: Apr 15, 1993|
|Structure viewer||Molecule: |
Downloads & links
E: cAMP-DEPENDENT PROTEIN KINASE
I: PEPTIDE INHIBITOR PKI(5-24)
|Atom site foot note||1: RESIDUES THR E 197 AND SER E 338 ARE PHOSPHORYLATED.|
|#1: Protein/peptide|| |
Mass: 40657.316 Da / Num. of mol.: 1 / Source: (gene. exp.) Mus musculus (house mouse) / Genus: Mus / References: UniProt: P05132, EC: 188.8.131.52
|#2: Protein/peptide|| |
Mass: 2226.411 Da / Num. of mol.: 1 / Source: (gene. exp.) Mus musculus (house mouse) / Genus: Mus / References: UniProt: P63248
|#3: Chemical||#4: Chemical|| ChemComp-ATP / ||#5: Water|| ChemComp-HOH / ||Nonpolymer details||INHIBITOR RESIDUE NUMBERING CORRESPONDS TO NUMBERS FROM THE SEQUENCE OF THE LARGER NATURALLY ...INHIBITOR RESIDUE NUMBERING CORRESPOND|
|Experiment||Method: X-RAY DIFFRACTION|
|Crystal||Density Matthews: 2.65 / Density percent sol: 53.51 %|
*PLUSTemp: 4 ℃ / pH: 8 / Method: vapor diffusion, hanging drop / Details: Zheng, J., (1993) Biochemistry, 32, 2154.
|components of the solutions|
|Radiation||Scattering type: x-ray|
|Radiation wavelength||Relative weight: 1|
*PLUSD resolution high: 2.2 / Number obs: 21443 / Percent possible obs: 90.6 / Rmerge I obs: 0.069
*PLUSHighest resolution: 2.2 / Lowest resolution: 2.37 / Rmerge I obs: 0.079
|Refine||Sigma F: 2|
|Least-squares process||R factor R work: 0.177 / Highest resolution: 2.2 / Lowest resolution: 10|
|Refine hist #LAST||Highest resolution: 2.2 / Lowest resolution: 10|
|Number of atoms included #LAST||Protein: 2934 / Nucleic acid: 0 / Ligand: 33 / Solvent: 103 / Total: 3070|
|Refine LS restraints|
*PLUSName: XPLOR/TNT / Classification: refinement
*PLUSR factor all: 0.199 / R factor obs: 0.177
|Refine LS restraints|
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