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- PDB-1amf: CRYSTAL STRUCTURE OF MODA, A MOLYBDATE TRANSPORT PROTEIN, COMPLEX... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1amf | ||||||
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Title | CRYSTAL STRUCTURE OF MODA, A MOLYBDATE TRANSPORT PROTEIN, COMPLEXED WITH MOLYBDATE | ||||||
![]() | MOLYBDATE TRANSPORT PROTEIN MODA | ||||||
![]() | BINDING PROTEIN / MOLYBDATE TRANSPORT PROTEIN / MOLYBDATE / PERIPLASMIC | ||||||
Function / homology | ![]() response to chromate / tungstate ion transport / ABC-type molybdate transporter activity / molybdate ion binding / tungstate binding / molybdate ion transport / molybdenum ion binding / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / outer membrane-bounded periplasmic space / membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Hu, Y. / Rech, S. / Gunsalus, R.P. / Rees, D.C. | ||||||
![]() | ![]() Title: Crystal structure of the molybdate binding protein ModA. Authors: Hu, Y. / Rech, S. / Gunsalus, R.P. / Rees, D.C. #1: ![]() Title: Properties of the Periplasmic Moda Molybdate-Binding Protein of Escherichia Coli Authors: Rech, S. / Wolin, C. / Gunsalus, R.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 65.8 KB | Display | ![]() |
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PDB format | ![]() | 50 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 374.7 KB | Display | ![]() |
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Full document | ![]() | 374.3 KB | Display | |
Data in XML | ![]() | 6 KB | Display | |
Data in CIF | ![]() | 8.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 24948.264 Da / Num. of mol.: 1 / Fragment: N-DOMAIN, C-DOMAIN / Source method: isolated from a natural source Details: MOLYBDATE ANION IS SEQUESTERED BETWEEN N-AND C-DOMAINS Source: (natural) ![]() ![]() |
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#2: Chemical | ChemComp-MOO / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.1 Å3/Da / Density % sol: 61.73 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 4.7 Details: PROTEIN WAS CRYSTALLIZED FROM 27.5% PEG 8000, 0.1M SODIUM ACETATE, PH 4.7, AND 2MM SODIUM MOLYBDATE | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 300 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS / Detector: IMAGE PLATE / Date: Aug 1, 1996 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→30 Å / Num. obs: 30575 / % possible obs: 93.2 % / Redundancy: 3.7 % / Biso Wilson estimate: 24.9 Å2 / Rsym value: 0.055 / Net I/σ(I): 21 |
Reflection shell | Resolution: 1.75→1.81 Å / Redundancy: 1.4 % / Mean I/σ(I) obs: 1.9 / Rsym value: 0.244 / % possible all: 60.8 |
Reflection | *PLUS Num. measured all: 112116 / Rmerge(I) obs: 0.055 |
Reflection shell | *PLUS % possible obs: 60.8 % / Rmerge(I) obs: 0.244 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 28.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.75→30 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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