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- PDB-1ajr: REFINEMENT AND COMPARISON OF THE CRYSTAL STRUCTURES OF PIG CYTOSO... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ajr | ||||||
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Title | REFINEMENT AND COMPARISON OF THE CRYSTAL STRUCTURES OF PIG CYTOSOLIC ASPARTATE AMINOTRANSFERASE AND ITS COMPLEX WITH 2-METHYLASPARTATE | ||||||
![]() | ASPARTATE AMINOTRANSFERASE | ||||||
![]() | AMINOTRANSFERASE / CYTOSOLIC ASPARTATE AMINOTRANSFERASE / PIG / HOMODIMER IN THE ABSENCE OF LIGAND | ||||||
Function / homology | ![]() Aspartate and asparagine metabolism / Malate-aspartate shuttle / phosphatidylserine decarboxylase activity / glutamate catabolic process to aspartate / glutamate catabolic process to 2-oxoglutarate / cysteine transaminase / L-cysteine transaminase activity / glycerol biosynthetic process / aspartate catabolic process / aspartate biosynthetic process ...Aspartate and asparagine metabolism / Malate-aspartate shuttle / phosphatidylserine decarboxylase activity / glutamate catabolic process to aspartate / glutamate catabolic process to 2-oxoglutarate / cysteine transaminase / L-cysteine transaminase activity / glycerol biosynthetic process / aspartate catabolic process / aspartate biosynthetic process / aspartate metabolic process / glutamate metabolic process / 2-oxoglutarate metabolic process / aspartate transaminase / oxaloacetate metabolic process / L-aspartate:2-oxoglutarate aminotransferase activity / fatty acid homeostasis / response to glucocorticoid / Notch signaling pathway / gluconeogenesis / cellular response to insulin stimulus / pyridoxal phosphate binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Rhee, S. / Silva, M.M. / Hyde, C.C. / Rogers, P.H. / Metzler, C.M. / Metzler, D.E. / Arnone, A. | ||||||
![]() | ![]() Title: Refinement and comparisons of the crystal structures of pig cytosolic aspartate aminotransferase and its complex with 2-methylaspartate. Authors: Rhee, S. / Silva, M.M. / Hyde, C.C. / Rogers, P.H. / Metzler, C.M. / Metzler, D.E. / Arnone, A. #1: ![]() Title: Pig Cytosolic Aspartate Aminotransferase Authors: Arnone, A. / Rogers, P.H. / Hyde, C.C. / Briley, P.D. / Metzler, C.M. / Metzler, D.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 179.1 KB | Display | ![]() |
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PDB format | ![]() | 142.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.999731, 0.005529, 0.022518), Vector: |
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Components
#1: Protein | Mass: 46621.641 Da / Num. of mol.: 2 / Source method: isolated from a natural source Details: LYS258 SIDE CHAIN FORMS THE SCHIFF BASE WITH COENZYME PYRIDOXAL 5'-PHOSPHATE Source: (natural) ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 48 % | |||||||||||||||
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Crystal grow | pH: 5.4 / Details: 40MM SODIUM ACETATE (PH 5.4)/8% PEG6000 | |||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: Dec 1, 1990 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.74→100 Å / Num. obs: 91745 / % possible obs: 95.9 % / Observed criterion σ(I): 0 / Redundancy: 4.9 % / Biso Wilson estimate: 16.6 Å2 / Rsym value: 0.054 / Net I/σ(I): 12.1 |
Reflection shell | Resolution: 1.74→1.87 Å / Redundancy: 3.2 % / Mean I/σ(I) obs: 3.1 / Rsym value: 0.17 / % possible all: 79.7 |
Reflection | *PLUS Num. measured all: 444811 / Rmerge(I) obs: 0.054 |
Reflection shell | *PLUS % possible obs: 79.7 % / Rmerge(I) obs: 0.17 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 24.1 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.2 Å / Luzzati d res low obs: 8 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.74→8 Å
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Refine LS restraints |
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Software | *PLUS Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.17 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |