+Open data
-Basic information
Entry | Database: PDB / ID: 1a8p | ||||||
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Title | FERREDOXIN REDUCTASE FROM AZOTOBACTER VINELANDII | ||||||
Components | NADPH\:FERREDOXIN OXIDOREDUCTASE | ||||||
Keywords | OXIDOREDUCTASE | ||||||
Function / homology | Function and homology information ferredoxin-NADP+ reductase / ferredoxin-NADP+ reductase activity / nucleotide binding Similarity search - Function | ||||||
Biological species | Azotobacter vinelandii (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SIRAS / Resolution: 2 Å | ||||||
Authors | Prasad, G.S. / Kresge, N. / Muhlberg, A.B. / Shaw, A. / Jung, Y.S. / Burgess, B.K. / Stout, C.D. | ||||||
Citation | Journal: Protein Sci. / Year: 1998 Title: The crystal structure of NADPH:ferredoxin reductase from Azotobacter vinelandii. Authors: Sridhar Prasad, G. / Kresge, N. / Muhlberg, A.B. / Shaw, A. / Jung, Y.S. / Burgess, B.K. / Stout, C.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1a8p.cif.gz | 66.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1a8p.ent.gz | 48.5 KB | Display | PDB format |
PDBx/mmJSON format | 1a8p.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a8/1a8p ftp://data.pdbj.org/pub/pdb/validation_reports/a8/1a8p | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 29450.594 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Azotobacter vinelandii (bacteria) / Strain: LM100 / References: UniProt: Q44532 |
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#2: Chemical | ChemComp-FAD / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.84 % | |||||||||||||||||||||||||
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Crystal grow | pH: 5.5 / Details: pH 5.5 | |||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 22 ℃ / pH: 7.4 / Method: vapor diffusion, sitting dropDetails: drop consists of equal volume of protein and reservoir solutions | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 290 K |
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Diffraction source | Source: ROTATING ANODE / Type: MACSCIENCE / Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Feb 1, 1995 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 19076 / % possible obs: 99.8 % / Observed criterion σ(I): 0 / Redundancy: 5 % / Rsym value: 0.101 / Net I/σ(I): 6.8 |
Reflection shell | Resolution: 2→2.07 Å / Redundancy: 4.9 % / Mean I/σ(I) obs: 1.6 / Rsym value: 0.445 / % possible all: 99.3 |
Reflection | *PLUS Rmerge(I) obs: 0.101 |
Reflection shell | *PLUS % possible obs: 99.3 % / Rmerge(I) obs: 0.445 |
-Processing
Software |
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Refinement | Method to determine structure: SIRAS / Resolution: 2→8 Å / σ(F): 2
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Displacement parameters | Biso mean: 19.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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