SOLUTION NMR STRUCTURE OF TUMOR SUPPRESSOR P16INK4A, 18 STRUCTURES
Components
TUMOR SUPPRESSOR P16INK4A
Keywords
ANTI-ONCOGENE / CELL CYCLE / ANK REPEAT
Function / homology
Function and homology information
senescence-associated heterochromatin focus / positive regulation of macrophage apoptotic process / Evasion of Oncogene Induced Senescence Due to Defective p16INK4A binding to CDK4 / Evasion of Oxidative Stress Induced Senescence Due to Defective p16INK4A binding to CDK4 / Evasion of Oncogene Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / Evasion of Oxidative Stress Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / positive regulation of smooth muscle cell apoptotic process / negative regulation of cyclin-dependent protein serine/threonine kinase activity / negative regulation of phosphorylation / cyclin-dependent protein serine/threonine kinase inhibitor activity ...senescence-associated heterochromatin focus / positive regulation of macrophage apoptotic process / Evasion of Oncogene Induced Senescence Due to Defective p16INK4A binding to CDK4 / Evasion of Oxidative Stress Induced Senescence Due to Defective p16INK4A binding to CDK4 / Evasion of Oncogene Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / Evasion of Oxidative Stress Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / positive regulation of smooth muscle cell apoptotic process / negative regulation of cyclin-dependent protein serine/threonine kinase activity / negative regulation of phosphorylation / cyclin-dependent protein serine/threonine kinase inhibitor activity / negative regulation of cell-matrix adhesion / negative regulation of NF-kappaB transcription factor activity / regulation of G1/S transition of mitotic cell cycle / Transcriptional Regulation by VENTX / NF-kappaB binding / replicative senescence / Oncogene Induced Senescence / negative regulation of cell growth / Cyclin D associated events in G1 / cellular senescence / Senescence-Associated Secretory Phenotype (SASP) / Oxidative Stress Induced Senescence / Ras protein signal transduction / regulation of cell cycle / cell cycle / negative regulation of cell population proliferation / negative regulation of DNA-templated transcription / protein kinase binding / RNA binding / nucleus / cytoplasm / cytosol Similarity search - Function
CLOSEST TO MEAN STRUCTURE WHICH SHOWS GOOD AGREEMENT WITH THE CONSTRAINTS. NONE OF THE CONSTRAINTS SHOW NOE VIOLATION BIGGER THAN 0.5 A AND DIHEDRAL ANGLE VIOLATION BIGGER THAN 5 DEGREE.
Representative
-
Components
#1: Protein
TUMORSUPPRESSORP16INK4A
Mass: 16554.641 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: TUMOR SUPPRESSOR P16INK4A / Source: (gene. exp.) Homo sapiens (human) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P42771
-
Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
NMR EXPERIMENTS FOR THE ASSIGNMENTS AND STRUCTURE CALCULATIONS : HN(CA)CB
1
2
1
HNCA
1
3
1
CBCA(CO)HN
1
4
1
HN(CO)CA
1
5
1
(H)CCH-TOCSY
1
6
1
HNHA
1
7
1
3D 15N-EDITED NOESY AND TOCSY
1
8
1
2D NOESY AND TOCSY
1
9
1
3D 13C-EDITED NOESY
1
10
1
3D 15N/13C SIMULTANEOUSLY EDITED NOESY
1
11
1
4D 15N/13C-EDITED NOESY
1
12
1
4D 13C/13C-EDITED NOESY.
NMR details
Text: THE SOLUTION STRUCTURE OF THE TUMOR SUPPRESSOR P16INK4A HAS BEEN DETERMINED BY MULTI-DIMENSIONAL HERERONUCLEAR NMR. THE STRUCTURES WERE CALCULATED USING THE SIMULATED ANNEALING PROTOCOL OF ...Text: THE SOLUTION STRUCTURE OF THE TUMOR SUPPRESSOR P16INK4A HAS BEEN DETERMINED BY MULTI-DIMENSIONAL HERERONUCLEAR NMR. THE STRUCTURES WERE CALCULATED USING THE SIMULATED ANNEALING PROTOCOL OF NILGES ET AL. (1988) FEBS LETT. 229, 129-136 USING THE PROGRAM X-PLOR 3.1 (BRUNGER). THE CALCULATION IS BASED ON 1437 EXPERIMENTAL NMR RESTRAINTS (1370 DISTANCE AND 67 TORSION ANGLE RESTRAINTS).
-
Sample preparation
Details
Contents: H2O AND D2O
Sample conditions
Ionic strength: CA. 0 / pH: 7.5 / Pressure: NO / Temperature: 293 K
Crystal grow
*PLUS
Method: other / Details: NMR
-
NMR measurement
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker DMX600
Bruker
DMX600
600
1
Bruker DRX800
Bruker
DRX800
800
2
-
Processing
Software
Name
Version
Classification
X-PLOR
3.1
modelbuilding
X-PLOR
3.1
refinement
X-PLOR
3.1
phasing
NMR software
Name
Classification
X-PLOR
structuresolution
X-PLOR
refinement
Refinement
Method: simulated annealing / Software ordinal: 1 Details: THE 18 STRUCTURES WERE SUPERIMPOSED IN ORDER TO HAVE BEST FITTING FOR THE BACKBONE ATOMS (N, CA, C) OF RESIDUES 14-134. THE EXPERIMENTAL RESTRAINTS ARE PRESENTED IN ENTRY 1INK.MR. RESIDUES 1- ...Details: THE 18 STRUCTURES WERE SUPERIMPOSED IN ORDER TO HAVE BEST FITTING FOR THE BACKBONE ATOMS (N, CA, C) OF RESIDUES 14-134. THE EXPERIMENTAL RESTRAINTS ARE PRESENTED IN ENTRY 1INK.MR. RESIDUES 1-13 AND 135-156 ARE POORLY DEFINED BY THE EXPERIMENTAL DATA. THUS, NO MEANING SHOULD BE GIVEN TO THOSE RESIDUES' COORDINATES.
NMR ensemble
Conformer selection criteria: CLOSEST TO MEAN STRUCTURE WHICH SHOWS GOOD AGREEMENT WITH THE CONSTRAINTS. NONE OF THE CONSTRAINTS SHOW NOE VIOLATION BIGGER THAN 0.5 A AND DIHEDRAL ANGLE VIOLATION BIGGER THAN 5 DEGREE. Conformers calculated total number: 90 / Conformers submitted total number: 18
+
About Yorodumi
-
News
-
Feb 9, 2022. New format data for meta-information of EMDB entries
New format data for meta-information of EMDB entries
Version 3 of the EMDB header file is now the official format.
The previous official version 1.9 will be removed from the archive.
In the structure databanks used in Yorodumi, some data are registered as the other names, "COVID-19 virus" and "2019-nCoV". Here are the details of the virus and the list of structure data.
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)
EMDB accession codes are about to change! (news from PDBe EMDB page)
The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
The EM Navigator/Yorodumi systems omit the EMD- prefix.
Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator
Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.
Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi