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Yorodumi- PDB-1a24: SOLUTION NMR STRUCTURE OF REDUCED DSBA FROM ESCHERICHIA COLI, FAM... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1a24 | ||||||
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Title | SOLUTION NMR STRUCTURE OF REDUCED DSBA FROM ESCHERICHIA COLI, FAMILY OF 20 STRUCTURES | ||||||
Components | DSBA | ||||||
Keywords | OXIDOREDUCTASE / THIOL-DISULFIDE OXIDOREDUCTASE / INTRODUCTION OF DISULFIDE BONDS / PROTEIN FOLDING / REDOX-ACTIVE CENTER | ||||||
Function / homology | Function and homology information cellular response to antibiotic / protein disulfide isomerase activity / protein-disulfide reductase activity / outer membrane-bounded periplasmic space / periplasmic space / oxidoreductase activity Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Schirra, H.J. / Renner, C. / Czisch, M. / Huber-Wunderlich, M. / Holak, T.A. / Glockshuber, R. | ||||||
Citation | Journal: Biochemistry / Year: 1998 Title: Structure of reduced DsbA from Escherichia coli in solution. Authors: Schirra, H.J. / Renner, C. / Czisch, M. / Huber-Wunderlich, M. / Holak, T.A. / Glockshuber, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1a24.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1a24.ent.gz | 988.2 KB | Display | PDB format |
PDBx/mmJSON format | 1a24.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a2/1a24 ftp://data.pdbj.org/pub/pdb/validation_reports/a2/1a24 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 21155.025 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: THZ2 / Cellular location: PERIPLASM / Gene: DSBA / Plasmid: PDSBA2 / Cellular location (production host): PERIPLASM / Gene (production host): DSBA / Production host: Escherichia coli (E. coli) / Strain (production host): THZ2 / References: UniProt: P24991, UniProt: P0AEG4*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED WITH TRIPLE-RESONANCE NMR SPECTROSCOPY AND MULTIDIMENSIONAL NMR EXPERIMENTS ON 13C/15N LABELED DSBA |
-Sample preparation
Details | Contents: 20 MM SODIUM PHOSPHATE IN H2O |
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Sample conditions | Ionic strength: 20mM / pH: 3.7 / Pressure: 1013 HPA / Temperature: 300 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
Software |
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NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: REPEATED SIMULATED ANNEALING WITH 2000 HIGH TEMPERATURE STEPS AT 1000K AND 4000 COOLING STEPS | ||||||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATION / Conformers calculated total number: 80 / Conformers submitted total number: 20 |