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Yorodumi- PDB-121p: STRUKTUR UND GUANOSINTRIPHOSPHAT-HYDROLYSEMECHANISMUS DES C-TERMI... -
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-Basic information
Entry | Database: PDB / ID: 121p | ||||||
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Title | STRUKTUR UND GUANOSINTRIPHOSPHAT-HYDROLYSEMECHANISMUS DES C-TERMINAL VERKUERZTEN MENSCHLICHEN KREBSPROTEINS P21-H-RAS | ||||||
Components | H-RAS P21 PROTEIN | ||||||
Keywords | ONCOGENE PROTEIN | ||||||
Function / homology | Function and homology information phospholipase C activator activity / GTPase complex / oncogene-induced cell senescence / positive regulation of ruffle assembly / negative regulation of GTPase activity / regulation of neurotransmitter receptor localization to postsynaptic specialization membrane / positive regulation of miRNA metabolic process / T-helper 1 type immune response / positive regulation of wound healing / defense response to protozoan ...phospholipase C activator activity / GTPase complex / oncogene-induced cell senescence / positive regulation of ruffle assembly / negative regulation of GTPase activity / regulation of neurotransmitter receptor localization to postsynaptic specialization membrane / positive regulation of miRNA metabolic process / T-helper 1 type immune response / positive regulation of wound healing / defense response to protozoan / Signaling by RAS GAP mutants / Signaling by RAS GTPase mutants / Activation of RAS in B cells / RAS signaling downstream of NF1 loss-of-function variants / SOS-mediated signalling / Activated NTRK3 signals through RAS / Activated NTRK2 signals through RAS / SHC1 events in ERBB4 signaling / positive regulation of protein targeting to membrane / Signalling to RAS / SHC-related events triggered by IGF1R / Activated NTRK2 signals through FRS2 and FRS3 / adipose tissue development / SHC-mediated cascade:FGFR2 / Estrogen-stimulated signaling through PRKCZ / SHC-mediated cascade:FGFR3 / MET activates RAS signaling / Signaling by PDGFRA transmembrane, juxtamembrane and kinase domain mutants / Signaling by PDGFRA extracellular domain mutants / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / Schwann cell development / SHC-mediated cascade:FGFR4 / Signaling by FGFR4 in disease / Erythropoietin activates RAS / SHC-mediated cascade:FGFR1 / FRS-mediated FGFR2 signaling / protein-membrane adaptor activity / FRS-mediated FGFR3 signaling / Signaling by FLT3 ITD and TKD mutants / Signaling by FGFR2 in disease / FRS-mediated FGFR4 signaling / p38MAPK events / Signaling by FGFR3 in disease / Tie2 Signaling / FRS-mediated FGFR1 signaling / GRB2 events in EGFR signaling / FLT3 Signaling / SHC1 events in EGFR signaling / EGFR Transactivation by Gastrin / Signaling by FLT3 fusion proteins / EPHB-mediated forward signaling / Signaling by FGFR1 in disease / myelination / Ras activation upon Ca2+ influx through NMDA receptor / GRB2 events in ERBB2 signaling / intrinsic apoptotic signaling pathway / CD209 (DC-SIGN) signaling / NCAM signaling for neurite out-growth / SHC1 events in ERBB2 signaling / Downstream signal transduction / Constitutive Signaling by Overexpressed ERBB2 / Insulin receptor signalling cascade / positive regulation of epithelial cell proliferation / Signaling by phosphorylated juxtamembrane, extracellular and kinase domain KIT mutants / positive regulation of GTPase activity / small monomeric GTPase / VEGFR2 mediated cell proliferation / G protein activity / regulation of actin cytoskeleton organization / animal organ morphogenesis / FCERI mediated MAPK activation / positive regulation of JNK cascade / Signaling by ERBB2 TMD/JMD mutants / RAF activation / positive regulation of MAP kinase activity / Signaling by high-kinase activity BRAF mutants / regulation of long-term neuronal synaptic plasticity / Constitutive Signaling by EGFRvIII / MAP2K and MAPK activation / Signaling by ERBB2 ECD mutants / Signaling by SCF-KIT / Signaling by ERBB2 KD Mutants / cellular response to gamma radiation / positive regulation of type II interferon production / Regulation of RAS by GAPs / endocytosis / RAS processing / Negative regulation of MAPK pathway / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / chemotaxis / Signaling by BRAF and RAF1 fusions / GDP binding / cellular senescence / MAPK cascade / positive regulation of fibroblast proliferation / DAP12 signaling / insulin receptor signaling pathway Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.54 Å | ||||||
Authors | Krengel, U. / Scheffzek, K. / Scherer, A. / Kabsch, W. / Wittinghofer, A. / Pai, E.F. | ||||||
Citation | Journal: Thesis / Year: 1991 Title: Struktur Und Guanosintriphosphat-Hydrolysemechanismus Des C-Terminal Verkuerzten Menschlichen Krebsproteins P21-H-Ras Authors: Krengel, U. #1: Journal: Nato Asi Ser.,Ser.A / Year: 1991 Title: The Three-Dimensional Structure of P21 in the Catalytically Active Conformation and Analysis of Oncogenic Mutants Authors: Krengel, U. / Schlichting, I. / Scheidig, A. / Frech, M. / John, J. / Lautwein, A. / Wittinghofer, F. / Kabsch, W. / Pai, E.F. #2: Journal: Embo J. / Year: 1990 Title: Refined Crystal Structure of the Triphosphate Conformation of H-Ras P21 at 1.35 Angstroms Resolution: Implications for the Mechanism of GTP Hydrolysis Authors: Pai, E.F. / Krengel, U. / Petsko, G.A. / Goody, R.S. / Kabsch, W. / Wittinghofer, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 121p.cif.gz | 52.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb121p.ent.gz | 36.3 KB | Display | PDB format |
PDBx/mmJSON format | 121p.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 121p_validation.pdf.gz | 444.1 KB | Display | wwPDB validaton report |
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Full document | 121p_full_validation.pdf.gz | 446.8 KB | Display | |
Data in XML | 121p_validation.xml.gz | 5.7 KB | Display | |
Data in CIF | 121p_validation.cif.gz | 8.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/21/121p ftp://data.pdbj.org/pub/pdb/validation_reports/21/121p | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUES 61 - 64 (GLN - GLU - GLU - TYR) ADOPT SEVERAL CONFORMATIONS IN THE CRYSTAL. THE COORDINATES GIVEN APPROXIMATE ONE OF THESE. THE ELECTRON DENSITY FOR THIS PART OF THE STRUCTURE IS NOT AS ...1: RESIDUES 61 - 64 (GLN - GLU - GLU - TYR) ADOPT SEVERAL CONFORMATIONS IN THE CRYSTAL. THE COORDINATES GIVEN APPROXIMATE ONE OF THESE. THE ELECTRON DENSITY FOR THIS PART OF THE STRUCTURE IS NOT AS WELL DEFINED AS FOR THE REST OF THE STRUCTURE. |
-Components
#1: Protein | Mass: 18875.191 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / References: UniProt: P01112 |
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#2: Chemical | ChemComp-MG / |
#3: Chemical | ChemComp-GCP / |
#4: Water | ChemComp-HOH / |
Compound details | SECONDARY STRUCTURE ELEMENTS HAVE BEEN ASSIGNED ACCORDING TO THE PROGRAM DSSP (W.KABSCH AND C. ...SECONDARY STRUCTURE ELEMENTS HAVE BEEN ASSIGNED ACCORDING TO THE PROGRAM DSSP (W.KABSCH AND C.SANDER, 1983, BIOPOLYMER |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38.32 % |
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
-Processing
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Refinement | Rfactor Rwork: 0.195 / Rfactor obs: 0.195 / Highest resolution: 1.54 Å Details: RESIDUES 61 - 64 (GLN - GLU - GLU - TYR) ADOPT SEVERAL CONFORMATIONS IN THE CRYSTAL. THE COORDINATES GIVEN APPROXIMATE ONE OF THESE. THE ELECTRON DENSITY FOR THIS PART OF THE STRUCTURE IS ...Details: RESIDUES 61 - 64 (GLN - GLU - GLU - TYR) ADOPT SEVERAL CONFORMATIONS IN THE CRYSTAL. THE COORDINATES GIVEN APPROXIMATE ONE OF THESE. THE ELECTRON DENSITY FOR THIS PART OF THE STRUCTURE IS NOT AS WELL DEFINED AS FOR THE REST OF THE STRUCTURE. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 1.54 Å
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