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- PDB-11dg: Chimeric Escherichia coli 70S ribosome containing an evolved Vibr... -

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Basic information

Entry
Database: PDB / ID: 11dg
TitleChimeric Escherichia coli 70S ribosome containing an evolved Vibrio cholerae 16S rRNA (VC-S4.4)
Components
  • (30S ribosomal protein ...) x 2
  • (50S ribosomal protein ...) x 12
  • (Large ribosomal subunit protein ...) x 17
  • (Small ribosomal subunit protein ...) x 18
  • 16S ribosomal RNA
  • 23S ribosomal RNA
  • 5S ribosomal RNA
  • E-site tRNA
KeywordsRIBOSOME / Directed evolution / translation
Function / homology
Function and homology information


negative regulation of cytoplasmic translational initiation / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / positive regulation of ribosome biogenesis / RNA-binding transcription regulator activity / four-way junction DNA binding / negative regulation of cytoplasmic translation ...negative regulation of cytoplasmic translational initiation / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / positive regulation of ribosome biogenesis / RNA-binding transcription regulator activity / four-way junction DNA binding / negative regulation of cytoplasmic translation / DnaA-L2 complex / translation repressor activity / negative regulation of translational initiation / negative regulation of DNA-templated DNA replication initiation / mRNA regulatory element binding translation repressor activity / positive regulation of RNA splicing / cytosolic ribosome assembly / ribosome assembly / assembly of large subunit precursor of preribosome / transcription antitermination / DNA endonuclease activity / translational initiation / regulation of cell growth / response to radiation / DNA-templated transcription termination / maintenance of translational fidelity / mRNA 5'-UTR binding / large ribosomal subunit / ribosomal small subunit assembly / transferase activity / ribosome binding / ribosomal small subunit biogenesis / 5S rRNA binding / small ribosomal subunit / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / response to antibiotic / negative regulation of DNA-templated transcription / hydrolase activity / mRNA binding / DNA binding / RNA binding / zinc ion binding / membrane / cytoplasm / cytosol
Similarity search - Function
Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L31 type A / Ribosomal protein S21 superfamily / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L31 signature. ...Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L31 type A / Ribosomal protein S21 superfamily / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein S21 / Ribosomal protein L16 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L9 signature. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / : / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L28/L24 superfamily / : / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L9 / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L28 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein S19, bacterial-type / Ribosomal protein S13, bacterial-type / : / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L6, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S4, bacterial-type / Ribosomal protein L5, bacterial-type / Ribosomal protein S5, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / : / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L14P, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L35 / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L33 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 / Ribosomal protein S9, bacterial/plastid / Ribosomal L28 family / Ribosomal protein L33 superfamily / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein L28/L24 / Ribosomal protein L30, bacterial-type / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / L28p-like / Ribosomal protein S15, bacterial-type / Ribosomal protein L16
Similarity search - Domain/homology
SPERMIDINE / SPERMINE / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein bL28 ...SPERMIDINE / SPERMINE / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL33 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL36A / Large ribosomal subunit protein bL9 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein bL25
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3 Å
AuthorsRaskar, T. / Badran, A. / Fraser, J.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)GM145238 United States
National Institutes of Health/Office of the DirectorDP5OD024590 United States
CitationJournal: bioRxiv / Year: 2026
Title: Structural adaptations for enhanced translation kinetics in evolved ribosomes.
Authors: Tushar Raskar / Alan Costello / Ahmed H Badran / James S Fraser /
Abstract: The ribosomal RNA sequence governs translation dynamics, yet understanding how changes beyond the conserved catalytic centers influence kinetics and protein yield remains limited. Using orthogonal ...The ribosomal RNA sequence governs translation dynamics, yet understanding how changes beyond the conserved catalytic centers influence kinetics and protein yield remains limited. Using orthogonal ribosome phage-assisted continuous evolution (oRibo-PACE), we recently reported chimeric ribosomes derived from , , and endowed with elevated translation rates as compared to their starting counterparts. Here, we structurally characterize these kinetically enhanced ribosomes using cryo-electron microscopy and uncover a potential relationship between 16S rRNA stability and translation efficiency. Compared to their naive starting points, evolved ribosomes exhibit extensive RNA structural adaptation, often introduced by mismatches at key helical junctions, which leads to local RNA-protein rearrangements and destabilizes non-canonical base pairs. Compensatory mutations that restore base-pairing stability and eliminate flexibility reduced translational activity to wild-type levels. Across trajectories, increased translational output correlates with subtle, localized changes in the 16S rRNA sequence that introduce limited structural destabilization at specific elements. Taken together, our work provides new insights into rRNA structural malleability and establishes principles for engineering ribosomes with altered translation properties.
History
DepositionFeb 18, 2026Deposition site: RCSB / Processing site: RCSB
Revision 1.0Apr 8, 2026Provider: repository / Type: Initial release
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Revision 1.0Apr 8, 2026Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
0: 50S ribosomal protein L33
1: Large ribosomal subunit protein bL34
2: 50S ribosomal protein L35
3: Large ribosomal subunit protein bL36A
4: 50S ribosomal protein L31
5: E-site tRNA
A: 16S ribosomal RNA
B: Small ribosomal subunit protein uS2
C: 30S ribosomal protein S3
D: Small ribosomal subunit protein uS4
E: Small ribosomal subunit protein uS5
F: Small ribosomal subunit protein bS6
G: Small ribosomal subunit protein uS7
H: Small ribosomal subunit protein uS8
I: Small ribosomal subunit protein uS9
J: Small ribosomal subunit protein uS10
K: Small ribosomal subunit protein uS11
L: 30S ribosomal protein S12
M: Small ribosomal subunit protein uS13
N: Small ribosomal subunit protein uS14
O: Small ribosomal subunit protein uS15
P: Small ribosomal subunit protein bS16
Q: Small ribosomal subunit protein uS17
R: Small ribosomal subunit protein bS18
S: Small ribosomal subunit protein uS19
T: Small ribosomal subunit protein bS20
U: Small ribosomal subunit protein bS21
a: 23S ribosomal RNA
b: 5S ribosomal RNA
c: 50S ribosomal protein L2
d: 50S ribosomal protein L3
e: Large ribosomal subunit protein uL4
f: Large ribosomal subunit protein uL5
g: Large ribosomal subunit protein uL6
h: Large ribosomal subunit protein bL9
i: Large ribosomal subunit protein uL13
j: Large ribosomal subunit protein uL14
k: Large ribosomal subunit protein uL15
l: Large ribosomal subunit protein uL16
m: Large ribosomal subunit protein bL17
n: Large ribosomal subunit protein uL18
o: Large ribosomal subunit protein bL19
p: 50S ribosomal protein L20
q: Large ribosomal subunit protein bL21
r: Large ribosomal subunit protein uL22
s: 50S ribosomal protein L23
t: 50S ribosomal protein L24
u: Large ribosomal subunit protein bL25
v: 50S ribosomal protein L27
w: 50S ribosomal protein L28
x: Large ribosomal subunit protein uL29
y: 50S ribosomal protein L30
z: 50S ribosomal protein L32
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,142,103342
Polymers2,133,12553
Non-polymers8,978289
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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50S ribosomal protein ... , 12 types, 12 molecules 024cdpstvwyz

#1: Protein 50S ribosomal protein L33


Mass: 6388.631 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmG, rpmG_1 / Production host: Escherichia coli (E. coli) / References: UniProt: C3SME7
#3: Protein 50S ribosomal protein L35 / Large ribosomal subunit protein bL35 / Ribosomal protein A


Mass: 7313.032 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmI, b1717, JW1707 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7Q1
#5: Protein 50S ribosomal protein L31 / Large ribosomal subunit protein bL31-A


Mass: 7887.117 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmE, b3936, JW3907 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7M9
#30: Protein 50S ribosomal protein L2 / Large ribosomal subunit protein uL2


Mass: 29923.619 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplB, b3317, JW3279 / Production host: Escherichia coli (E. coli) / References: UniProt: P60422
#31: Protein 50S ribosomal protein L3


Mass: 22291.562 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / References: UniProt: C3SQU2
#43: Protein 50S ribosomal protein L20


Mass: 13528.024 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplT / Production host: Escherichia coli (E. coli) / References: UniProt: B7MAS6
#46: Protein 50S ribosomal protein L23


Mass: 11222.160 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rplW, rplW_1, A5U30_004552, A8502_004023, A9X72_02185, ACN81_25520, ACU57_20960, AM464_09220, AW118_24900, AWP47_12645, B6R15_004069, B6R31_004968, BANRA_04328, BCB93_004293, BER14_25190, BF481_ ...Gene: rplW, rplW_1, A5U30_004552, A8502_004023, A9X72_02185, ACN81_25520, ACU57_20960, AM464_09220, AW118_24900, AWP47_12645, B6R15_004069, B6R31_004968, BANRA_04328, BCB93_004293, BER14_25190, BF481_004930, BG944_001133, BGM66_003593, BJI68_08675, BJJ90_02125, BK292_20505, BK383_24645, BKL28_004543, BTB68_004356, BTQ06_10690, BvCmsKKP061_03030, BvCmsSIP010_02685, BXT93_05475, C0P57_002668, C1Q91_004904, C2121_004162, C2M16_25405, C2R31_004633, C3F40_19720, C9E67_02725, C9Z68_22635, CA593_09650, CF22_004849, CG692_20185, CG704_20870, CG831_003649, CIG67_10675, CQ986_004881, CR538_02420, CR539_21900, CR628_001366, CTR35_003630, CV83915_02780, CY655_21320, D1912_09370, D3C88_31150, D3G36_23050, D4M65_20655, D4N09_20255, D9D43_22550, D9E49_05240, D9J61_16735, DD762_23630, DIV22_15175, DNQ45_03840, DNX30_25395, DQ87_004776, DS732_24345, DTL43_20915, DU321_12085, E4K51_21575, E5H86_24440, E6D34_21675, EAI46_07560, EC95NR1_02709, ECs4183, EEP55_004492, EIA08_24040, EIZ93_12385, EN85_004349, EPS76_07130, EPS97_20010, EWK56_25080, ExPECSC038_03640, F7F11_22640, F7N46_23925, F9407_19210, F9413_21150, F9461_25570, F9B07_24600, FEJ01_22430, FIJ20_21110, FJQ40_18390, FKO60_25720, FOI11_019175, FOI11_04005, FPI65_20390, FPS11_25345, FTV92_20630, FV293_22380, FVB16_04955, FWK02_12650, FZN31_02550, FZU14_21905, G3V95_19930, G3W53_21040, G4A38_21595, G4A47_20865, G5603_24670, G8C01_22470, G9448_16990, GAI89_24745, GAJ12_24635, GGB84_004451, GJ11_21470, GKF66_21630, GNW61_16715, GOP25_22790, GP711_23390, GP954_00860, GP965_04380, GP975_01270, GP979_01920, GQA06_03865, GQM04_10650, GQM13_25285, GQM21_11315, GQN34_23350, GQW07_21465, GRO95_20730, GRW05_09145, GRW24_04670, GRW56_00380, GRW57_03455, GTP92_23110, GUC01_21265, H0O72_19500, HCQ42_003932, HEP30_018720, HEP34_004754, HHH44_004519, HI055_004156, HIE29_005203, HJQ60_004995, HLX92_09990, HLZ50_22095, HMV95_19690, HV109_02105, HV146_21755, HV209_14630, HVW04_17450, HVW43_18585, HVY77_02090, HVZ29_19180, HX086_19490, HX136_02110, I0E88_004501, I6H00_20840, I6H02_12075, IFB95_004822, IUC12_004844, J0541_004413, J5U05_004071, JNP96_25390, KV259_004342, KV317_004314, KV371_004328, KV406_004321, KV449_004356, KV455_004319, KV463_004341, KV469_004334, KV499_004405, KV500_004292, NCTC10082_02823, NCTC10089_00486, NCTC10090_03511, NCTC10418_00674, NCTC10429_01077, NCTC10764_03847, NCTC10767_01500, NCTC10865_00642, NCTC10974_00533, NCTC11112_01052, NCTC11124_04730, NCTC11126_02754, NCTC11181_02722, NCTC11341_01905, NCTC12950_00463, NCTC13148_03456, NCTC4450_02459, NCTC7927_00535, NCTC7928_02533, NCTC8009_01493, NCTC8179_05925, NCTC8333_00473, NCTC8500_00318, NCTC8621_00492, NCTC8622_00522, NCTC8959_03277, NCTC8960_03054, NCTC8985_04965, NCTC9001_04236, NCTC9044_01376, NCTC9045_00549, NCTC9073_00398, NCTC9075_00708, NCTC9081_00901, NCTC9117_00740, NCTC9702_00521, NCTC9706_02721, NCTC9962_06781, NEP60_26375, O5851_12245, RG28_21695, SAMEA3752557_01968, TUM18780_04200
Production host: Escherichia coli (E. coli) / References: UniProt: C3SQV2
#47: Protein 50S ribosomal protein L24


Mass: 11339.250 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rplX, A8502_004032, A9X72_02230, ACN81_25475, ACU57_21005, AM464_09175, AW118_24855, AWP47_12690, B6R15_004078, B6R31_004977, BANRA_04319, BCB93_004284, BER14_25145, BF481_004939, BG944_001124, ...Gene: rplX, A8502_004032, A9X72_02230, ACN81_25475, ACU57_21005, AM464_09175, AW118_24855, AWP47_12690, B6R15_004078, B6R31_004977, BANRA_04319, BCB93_004284, BER14_25145, BF481_004939, BG944_001124, BGM66_003602, BJI68_08720, BJJ90_02170, BK292_20460, BK383_24690, BKL28_004552, BTB68_004365, BTQ06_10645, BvCmsKKP061_03021, BvCmsSIP010_02676, BXT93_05520, C0P57_002659, C1Q91_004913, C2121_004171, C2M16_25450, C2R31_004624, C3F40_19765, C9E67_02770, C9Z68_22590, CA593_09695, CF22_004858, CG704_20915, CG831_003640, CIG67_10720, CQ986_004890, CR538_02465, CR539_21855, CR628_001357, CTR35_003621, CV83915_02789, CY655_21275, D4M65_20610, D9D43_22595, DD762_23585, DIV22_15220, DNQ45_03795, DNX30_25440, DQ87_004785, DS732_24300, DTL43_20960, DU321_12040, E4K51_21530, E5H86_24485, E6D34_21720, EAI46_07515, EC95NR1_02700, ECs4174, EEP55_004501, EIA08_23995, EIZ93_12430, EN85_004358, EPS97_20055, ExPECSC038_03649, F7F11_22685, F7N46_23970, F9407_19255, F9413_21195, F9461_25615, F9B07_24645, FEJ01_22475, FIJ20_21155, FJQ40_18345, FKO60_25675, FOI11_019220, FOI11_03960, FPS11_25390, FTV92_20675, FTV93_20860, FV293_22425, FVB16_04910, FWK02_06310, FZN31_02595, FZU14_21860, G3V95_19885, G4A38_21550, G4A47_20820, G5603_24625, G8C01_22515, G9448_17035, GAI89_24790, GAJ12_24680, GJ11_21425, GKF66_21585, GNW61_16670, GOP25_22745, GP954_00905, GP965_04335, GP975_01225, GP979_01965, GQA06_03910, GQM04_10605, GQM13_25240, GQM21_11270, GQW07_21510, GRO95_20775, GRW05_09100, GRW24_04715, GRW56_00335, GRW57_03500, GTP92_23155, GUC01_21220, H0O72_19455, HCQ42_003941, HEP30_018675, HEP34_004763, HHH44_004528, HI055_004147, HJQ60_005004, HLX92_10035, HLZ50_22140, HMV95_19645, HV109_02150, HV146_21710, HV209_14675, HVW04_17495, HVW43_18630, HVY77_02135, HVZ29_19135, HX086_19445, HX136_02155, I0E88_004510, I6H00_20885, I6H02_12030, IUC12_004853, J0541_004404, J5U05_004080, JNP96_25345, KV259_004351, KV317_004323, KV371_004337, KV406_004330, KV449_004365, KV455_004328, KV463_004350, KV469_004343, KV499_004414, KV500_004301, NCTC10082_02832, NCTC10418_00684, NCTC10764_03856, NCTC10865_00652, NCTC10974_00542, NCTC11124_04721, NCTC11181_02731, NCTC11341_01896, NCTC12950_00472, NCTC7922_04049, NCTC7927_00544, NCTC8009_01504, NCTC8179_05935, NCTC8333_00482, NCTC8500_00327, NCTC8621_00501, NCTC8622_00513, NCTC8960_03063, NCTC8985_04975, NCTC9044_01365, NCTC9045_00558, NCTC9077_00597, NCTC9117_00749, NCTC9702_00530, NCTC9706_02730, NEP60_26330, O5851_12290, RG28_21650, SAMEA3752557_01959, TUM18780_04290, WR15_19645
Production host: Escherichia coli (E. coli) / References: UniProt: C3SQZ7
#49: Protein 50S ribosomal protein L27 / Large ribosomal subunit protein bL27


Mass: 9146.540 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmA, b3185, JW3152 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7L8
#50: Protein 50S ribosomal protein L28


Mass: 9027.551 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmB, ECVG_03476 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A1X3JA73
#52: Protein 50S ribosomal protein L30


Mass: 6554.820 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rpmD, A5U30_004536, A6592_25715, A8499_003316, A8502_004039, A9X72_02265, AC065_15925, ACN81_25440, ACU57_21040, AM464_09140, APX88_16155, AW118_24820, AWP47_12725, B6R12_002823, B6R15_004085, ...Gene: rpmD, A5U30_004536, A6592_25715, A8499_003316, A8502_004039, A9X72_02265, AC065_15925, ACN81_25440, ACU57_21040, AM464_09140, APX88_16155, AW118_24820, AWP47_12725, B6R12_002823, B6R15_004085, B6R17_004958, B6R31_004984, B6R48_005051, B6R87_004961, BANRA_04312, BB540_01660, BER14_25110, BF481_004946, BG944_001117, BGM66_003609, BHS81_19755, BJI68_08755, BJJ90_02205, BK292_20425, BK383_24725, BKL28_004559, BMT50_12295, BMT91_19990, BR158_003774, BTB68_004372, BTQ06_10610, BUO55_004713, BvCmsKKP061_03014, BvCmsSIP010_02669, BXT93_05555, BZL69_25950, C0P57_002652, C1Q91_004920, C2121_004178, C2M16_25485, C2R31_004617, C3F40_19800, C5N07_23570, C9E67_02805, C9Z68_22555, CA593_09730, CCS08_00135, CCU84_002918, CDL36_25590, CF22_004865, CG692_20265, CG704_20950, CG831_003633, CIG67_10755, CQ986_004897, CQB02_00630, CR538_02500, CR539_21820, CR628_001350, CRM83_22925, CTR35_003614, CV83915_02796, CWS33_24860, CX938_003815, CY655_21240, D1912_11425, D3G36_23130, D4M65_20575, D4N09_20175, D6T60_05695, D9D43_22630, D9E34_23605, D9E49_05160, D9H94_20830, D9J03_20055, D9J61_16655, DD762_23550, DIV22_15255, DNQ45_03760, DNX30_25475, DS732_24265, DTL43_20995, DTL90_24695, DTM45_24550, DU321_12005, E0I52_20145, E4K51_21495, E5H86_24520, E6D34_21755, EA239_22795, EAI46_07480, EB534_RS01705, EC95NR1_02693, ECs4167, EIA08_23960, EIZ93_12465, EN85_004365, EPS76_07210, EPS97_20090, ERS085406_04406, ERS139208_04354, EWK56_25000, ExPECSC038_03656, F3P10_05565, F7F11_22720, F7N46_24005, F9413_21230, F9461_25650, F9B07_24680, FA868_22750, FEJ01_22510, FFF58_25450, FGG80_25130, FHD44_21270, FHQ97_20115, FIJ20_21190, FJQ40_18310, FKO60_25640, FN993_004711, FOI11_019255, FOI11_03925, FPI65_20310, FPS11_25425, FTV92_20710, FTV93_20895, FV293_22460, FVB16_04875, FWK02_06275, FZN31_02630, FZU14_21825, G3V95_19850, G3W53_20960, G4A38_21515, G4A47_20785, G5603_24590, G9448_17070, GAI89_24825, GAJ12_24715, GAJ26_22015, GGB84_004467, GIB53_19395, GJ11_21390, GKF52_21245, GKF66_21550, GKF89_18475, GNW61_16635, GOP14_004273, GOP25_22710, GP711_23310, GP954_00940, GP965_04300, GP975_01190, GP979_02000, GQA06_03945, GQM04_10570, GQM13_25205, GQM17_19920, GQM21_11235, GQN34_23270, GQW07_21545, GRO95_20810, GRW05_09065, GRW24_04750, GRW56_00300, GRW57_03535, GSM54_23325, GSY44_20005, GUC01_21185, GUI33_20050, H0O53_20825, H0O72_19420, H0P11_20920, H5Z35_004789, H6Y26_003164, HCQ42_003948, HEP30_018640, HEP34_004770, HHH44_004535, HI055_004140, HIE29_005187, HJY54_004753, HKA49_005035, HLQ92_22595, HLV18_23335, HLX92_10070, HLZ50_22175, HMV95_19610, HMW38_23110, HV109_02185, HV146_21675, HV209_14710, HVV39_09270, HVW04_17530, HVW43_18665, HVY77_02170, HVZ29_19100, HVZ30_02050, HVZ71_02355, HX136_02190, I6H00_20920, I6H02_11995, IFB95_004806, IUD45_003540, J0541_004397, J4S20_004802, J5U05_004087, J7T50_001453, JFD_01884, JFD_03713, JNP96_25310, KQ660_11775, NCTC10082_02839, NCTC10089_00502, NCTC10090_03527, NCTC10418_00690, NCTC10429_01095, NCTC10487_00445, NCTC10764_03863, NCTC10767_01516, NCTC10865_00661, NCTC10974_00551, NCTC11126_02771, NCTC11127_03223, NCTC11181_02738, NCTC11341_01889, NCTC12950_00479, NCTC13127_00786, NCTC4450_02475, NCTC7922_04042, NCTC7927_00551, NCTC7928_02516, NCTC8009_01511, NCTC8179_05942, NCTC8333_00489, NCTC8450_04937, NCTC8500_00335, NCTC8621_00508, NCTC8622_00507, NCTC8959_03261, NCTC8960_03070, NCTC8985_04982, NCTC9001_04254, NCTC9044_01356, NCTC9045_00566, NCTC9050_03611, NCTC9071_00483, NCTC9073_00380, NCTC9075_00724, NCTC9077_00604, NCTC9081_00918, NCTC9111_00867, NCTC9117_00756, NCTC9702_00537, NCTC9706_02737, NCTC9775_04003, NCTC9777_01891, NEP60_26295, OS110_25120, RG28_21615, SAMEA3472044_01162, SAMEA3472056_05411, SAMEA3752557_01952, SAMEA3753106_04634, TUM18780_04360, WR15_19610
Production host: Escherichia coli (E. coli) / References: UniProt: C3SR32
#53: Protein 50S ribosomal protein L32


Mass: 6463.445 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rpmF, rpmF_1, A5U30_002830, A8502_000661, A9X72_15435, ACN81_09130, ACU57_04225, AM464_23480, AW118_12110, AWP47_13180, B6R15_001792, B6R31_003138, BANRA_01624, BCB93_002043, BER14_10210, BF481_ ...Gene: rpmF, rpmF_1, A5U30_002830, A8502_000661, A9X72_15435, ACN81_09130, ACU57_04225, AM464_23480, AW118_12110, AWP47_13180, B6R15_001792, B6R31_003138, BANRA_01624, BCB93_002043, BER14_10210, BF481_002131, BG944_003607, BGM66_002729, BJI68_20640, BJJ90_15780, BK292_02505, BK383_03640, BKL28_002219, BTB68_003153, BTQ06_08080, BvCmsKKP061_02173, BvCmsSIP010_04163, BXT93_21825, C0P57_001747, C1Q91_003167, C2121_002257, C2M16_23600, C2R31_002348, C3F40_06530, C9E67_18185, C9Z68_07340, CA593_23040, CF22_003088, CG692_05970, CG704_04105, CG831_001542, CIG67_21935, CQ986_001084, CR538_15635, CR539_10135, CR628_002785, CTR35_002374, CV83915_00494, CY655_06595, D1912_08350, D3C88_19355, D3G36_11860, D4M65_04795, D4N09_11015, D9D43_11825, D9E49_23135, D9J61_04035, DD762_03625, DIV22_29035, DNQ45_10015, DNX30_12140, DQ87_002042, DS732_10505, DTL43_16270, DU321_22840, E4K51_18340, E5H86_12535, E6D34_10935, EAI46_03975, EC95NR1_05485, ECs1467, EEP55_000822, EIA08_02925, EIZ93_13150, EN85_002840, EPS76_00770, EPS97_06370, EWK56_20060, ExPECSC038_03490, F7F11_10320, F7N46_02450, F9413_18150, F9461_13585, F9B07_16900, FEJ01_12565, FIJ20_07300, FJQ40_00940, FKO60_17370, FOI11_007500, FOI11_14470, FPI65_06540, FPS11_20235, FTV92_08325, FTV93_08485, FV293_19565, FVB16_04695, FWK02_15570, FZN31_14620, FZU14_20010, G3V95_08050, G3W53_15755, G4A38_16050, G4A47_15410, G5603_00600, G8C01_10030, G9448_05130, GAI89_13280, GAJ12_13930, GGB84_000483, GJ11_06555, GKF66_05180, GNW61_10640, GOP25_17405, GP711_16475, GP954_12815, GP965_04045, GP975_03585, GP979_13150, GQA06_08430, GQM04_02110, GQM13_14340, GQM21_06935, GQN34_10245, GQW07_08880, GRO95_10420, GRW05_02410, GRW24_27585, GRW56_06550, GRW57_07595, GTP92_19120, GUC01_01680, H0O72_16850, HCQ42_001914, HEP30_011955, HEP34_001075, HHH44_003019, HI055_001485, HIE29_000052, HJQ60_005092, HLX92_03690, HLZ50_01830, HMV95_16370, HV109_14610, HV146_09250, HV209_29060, HVV39_00410, HVW04_05590, HVW43_06430, HVY77_15765, HVZ29_08715, HX086_07035, HX136_15650, I0E88_003516, I6H00_08540, I6H02_24530, IFB95_003696, IUC12_000121, J0541_003169, J5U05_002959, JNP96_10710, KV259_001392, KV317_003230, KV371_000953, KV406_001102, KV449_001342, KV455_003227, KV463_001049, KV469_000542, KV499_000679, KV500_001199, NCTC10082_00641, NCTC10089_03005, NCTC10090_00680, NCTC10418_04509, NCTC10764_02647, NCTC10767_05044, NCTC10865_03684, NCTC10974_03416, NCTC11126_00919, NCTC11181_00164, NCTC11341_04894, NCTC12950_03296, NCTC13148_05706, NCTC4450_04127, NCTC7922_00634, NCTC7927_03385, NCTC7928_00455, NCTC8009_05657, NCTC8179_02874, NCTC8333_03590, NCTC8621_03238, NCTC8622_05813, NCTC8959_00826, NCTC8960_00763, NCTC8985_01747, NCTC9044_04397, NCTC9045_03550, NCTC9073_01975, NCTC9075_04022, NCTC9077_03763, NCTC9081_03511, NCTC9117_03837, NCTC9706_00412, NCTC9962_06984, NEP60_22810, O5851_21660, RG28_16890, SAMEA3752557_01555, TUM18780_25170, WR15_23855
Production host: Escherichia coli (E. coli) / References: UniProt: C3TE02

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Large ribosomal subunit protein ... , 17 types, 17 molecules 13efghijklmnoqrux

#2: Protein/peptide Large ribosomal subunit protein bL34 / 50S ribosomal protein L34


Mass: 5397.463 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmH, rimA, ssaF, b3703, JW3680 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7P5
#4: Protein/peptide Large ribosomal subunit protein bL36A / 50S ribosomal protein L36 / Ribosomal protein B


Mass: 4377.390 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmJ, b3299, JW3261 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7Q6
#32: Protein Large ribosomal subunit protein uL4 / 50S ribosomal protein L4


Mass: 22121.566 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplD, eryA, b3319, JW3281 / Production host: Escherichia coli (E. coli) / References: UniProt: P60723
#33: Protein Large ribosomal subunit protein uL5 / 50S ribosomal protein L5


Mass: 20333.611 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplE, b3308, JW3270 / Production host: Escherichia coli (E. coli) / References: UniProt: P62399
#34: Protein Large ribosomal subunit protein uL6 / 50S ribosomal protein L6


Mass: 18932.791 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplF, b3305, JW3267 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AG55
#35: Protein Large ribosomal subunit protein bL9 / 50S ribosomal protein L9


Mass: 15789.020 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplI, b4203, JW4161 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7R1
#36: Protein Large ribosomal subunit protein uL13 / 50S ribosomal protein L13


Mass: 16050.606 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplM, b3231, JW3200 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AA10
#37: Protein Large ribosomal subunit protein uL14 / 50S ribosomal protein L14


Mass: 13565.067 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplN, b3310, JW3272 / Production host: Escherichia coli (E. coli) / References: UniProt: P0ADY3
#38: Protein Large ribosomal subunit protein uL15 / 50S ribosomal protein L15


Mass: 15008.471 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplO, b3301, JW3263 / Production host: Escherichia coli (E. coli) / References: UniProt: P02413
#39: Protein Large ribosomal subunit protein uL16 / 50S ribosomal protein L16


Mass: 15343.327 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplP, EcE24377A_3796 / Production host: Escherichia coli (E. coli) / References: UniProt: A7ZSK2
#40: Protein Large ribosomal subunit protein bL17 / 50S ribosomal protein L17


Mass: 14393.657 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplQ, b3294, JW3256 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AG44
#41: Protein Large ribosomal subunit protein uL18 / 50S ribosomal protein L18


Mass: 12794.668 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplR, b3304, JW3266 / Production host: Escherichia coli (E. coli) / References: UniProt: P0C018
#42: Protein Large ribosomal subunit protein bL19


Mass: 13159.278 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplS / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7K8
#44: Protein Large ribosomal subunit protein bL21 / 50S ribosomal protein L21


Mass: 11586.374 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplU, b3186, JW3153 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AG48
#45: Protein Large ribosomal subunit protein uL22 / 50S ribosomal protein L22


Mass: 12253.359 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplV, eryB, b3315, JW3277 / Production host: Escherichia coli (E. coli) / References: UniProt: P61175
#48: Protein Large ribosomal subunit protein bL25 / 50S ribosomal protein L25


Mass: 10713.465 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplY, b2185, JW2173 / Production host: Escherichia coli (E. coli) / References: UniProt: P68919
#51: Protein Large ribosomal subunit protein uL29


Mass: 7286.464 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rpmC, A5U30_004546, A8502_004029, ACN81_25490, ACU57_20990, AW118_24870, AWP47_12675, B6R15_004075, B6R31_004974, BANRA_01943, BANRA_04322, BE932_15505, BER14_25160, BG944_001127, BGM66_003599, ...Gene: rpmC, A5U30_004546, A8502_004029, ACN81_25490, ACU57_20990, AW118_24870, AWP47_12675, B6R15_004075, B6R31_004974, BANRA_01943, BANRA_04322, BE932_15505, BER14_25160, BG944_001127, BGM66_003599, BGZ_02596, BGZ_04119, BJI68_08705, BK292_20475, BK383_24675, BKL28_004549, BTB68_004362, BTQ06_10660, BvCmsKKP061_03024, BvCmsSIP010_02679, BXT93_05505, C0P57_002662, C1Q91_004910, C2121_004168, C2M16_25435, C2R31_004627, C3F40_19750, C9Z68_22605, CF22_004855, CG704_20900, CG831_003643, CIG67_10705, CTR35_003624, CV83915_02786, D3G36_23080, D4M65_20625, D4N09_20225, D9D43_22580, D9E49_05210, D9J61_16705, DD762_23600, DIV22_15205, DNQ45_03810, DNX30_25425, DS732_24315, DTL43_20945, DU321_12055, E4K51_21545, E5H86_24470, E6D34_21705, EAI46_07530, ECs4177, EIA08_24010, EIMP300_17750, EIZ93_12415, EN85_004355, EPS76_07160, EPS97_20040, EWK56_25050, ExPECSC038_03646, F7F11_22670, F7N46_23955, F9413_21180, F9461_25600, F9B07_24630, FGAF848_25910, FIJ20_21140, FJQ40_18360, FKO60_25690, FOI11_019205, FOI11_03975, FORC44_0486, FPI65_20360, FPS11_25375, FTV93_20845, FVB16_04925, FWK02_06325, FZU14_21875, G3V95_19900, G3W53_21010, G4A38_21565, G4A47_20835, G5603_24640, GAI89_24775, GAJ12_24665, GKF66_21600, GNW61_16685, GOP25_22760, GP711_23360, GP954_00890, GP965_04350, GP975_01240, GP979_01950, GQA06_03895, GQE86_20135, GQM04_10620, GQM13_25255, GQM21_11285, GQN34_23320, GQW07_21495, GRC73_21695, GRO95_20760, GRW05_09115, GRW24_04700, GRW56_00350, GRW57_03485, GUC01_21235, H0O72_19470, HEP30_018690, HEP34_004760, HHH44_004525, HI055_004150, HIE29_005197, HJQ60_005001, HLX92_10020, HLZ50_22125, HMV95_19660, HV109_02135, HV209_14660, HVV39_09320, HVW04_17480, HVW43_18615, HVY77_02120, I6H00_20870, I6H02_12045, IFB95_004816, J0541_004407, J5U05_004077, JNP96_25360, KV499_004411, NCTC10082_02829, NCTC10089_00492, NCTC10418_00681, NCTC10429_01084, NCTC10764_03853, NCTC10767_01506, NCTC10865_00649, NCTC10974_00539, NCTC11126_02760, NCTC11181_02728, NCTC11341_01899, NCTC7922_04052, NCTC7927_00541, NCTC7928_02527, NCTC8009_01501, NCTC8179_05932, NCTC8333_00479, NCTC8500_00324, NCTC8621_00498, NCTC8622_00516, NCTC8959_03271, NCTC8960_03060, NCTC8985_04972, NCTC9001_04244, NCTC9044_01368, NCTC9045_00555, NCTC9075_00714, NCTC9077_00594, NCTC9081_00907, NCTC9117_00746, NCTC9702_00527, NCTC9706_02727, NCTC9962_06787, NEP60_26345, OGM49_22385, QDW62_02140, RZR61_12945, SAMEA3472112_00683, SAMEA3752557_01962, WR15_19660
Production host: Escherichia coli (E. coli) / References: UniProt: C3SQY2

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RNA chain , 4 types, 4 molecules 5Aab

#6: RNA chain E-site tRNA


Mass: 589.430 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli)
#7: RNA chain 16S ribosomal RNA


Mass: 491856.719 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli)
#28: RNA chain 23S ribosomal RNA


Mass: 941811.562 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli)
#29: RNA chain 5S ribosomal RNA


Mass: 38790.090 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / References: GenBank: 1273279017

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Small ribosomal subunit protein ... , 18 types, 18 molecules BDEFGHIJKMNOPQRSTU

#8: Protein Small ribosomal subunit protein uS2 / 30S ribosomal protein S2


Mass: 26781.670 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsB, b0169, JW0164 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7V0
#10: Protein Small ribosomal subunit protein uS4


Mass: 23514.199 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsD, ramA, b3296, JW3258 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7V8
#11: Protein Small ribosomal subunit protein uS5


Mass: 17629.398 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsE, spc, b3303, JW3265 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7W1
#12: Protein Small ribosomal subunit protein bS6


Mass: 15727.512 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsF, b4200, JW4158 / Production host: Escherichia coli (E. coli) / References: UniProt: P02358
#13: Protein Small ribosomal subunit protein uS7


Mass: 20055.156 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsG, b3341, JW3303 / Production host: Escherichia coli (E. coli) / References: UniProt: P02359
#14: Protein Small ribosomal subunit protein uS8


Mass: 14146.557 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsH / Production host: Escherichia coli (E. coli) / References: UniProt: C3SR12
#15: Protein Small ribosomal subunit protein uS9


Mass: 14886.270 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsI / Production host: Escherichia coli (E. coli) / References: UniProt: C3SRY2
#16: Protein Small ribosomal subunit protein uS10


Mass: 11755.597 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsJ / Production host: Escherichia coli (E. coli) / References: UniProt: C3SQT7
#17: Protein Small ribosomal subunit protein uS11 / 30S ribosomal protein S11


Mass: 13887.958 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsK, EcE24377A_3780 / Production host: Escherichia coli (E. coli) / References: UniProt: A7ZSI6
#19: Protein Small ribosomal subunit protein uS13


Mass: 13128.467 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsM, G711_03878 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A7U9IV78
#20: Protein Small ribosomal subunit protein uS14


Mass: 11606.560 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsN, NCTC9073_00385 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A2X1PQW4
#21: Protein Small ribosomal subunit protein uS15


Mass: 10290.816 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsO / Production host: Escherichia coli (E. coli) / References: UniProt: C3SSQ7
#22: Protein Small ribosomal subunit protein bS16 / 30S ribosomal protein S16


Mass: 9207.572 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsP, b2609, JW2590 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7T3
#23: Protein Small ribosomal subunit protein uS17


Mass: 9724.491 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsQ / Production host: Escherichia coli (E. coli) / References: UniProt: C3SQY7
#24: Protein Small ribosomal subunit protein bS18


Mass: 9005.472 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsR / Production host: Escherichia coli (E. coli) / References: UniProt: A0A0E2KXL3
#25: Protein Small ribosomal subunit protein uS19


Mass: 10455.355 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsS / Production host: Escherichia coli (E. coli) / References: UniProt: C3SQW2
#26: Protein Small ribosomal subunit protein bS20 / 30S ribosomal protein S20


Mass: 9712.476 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsT, EcE24377A_0023 / Production host: Escherichia coli (E. coli) / References: UniProt: A7ZHB2
#27: Protein Small ribosomal subunit protein bS21


Mass: 8524.039 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsU / Production host: Escherichia coli (E. coli) / References: UniProt: A0A0E2L2J1

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30S ribosomal protein ... , 2 types, 2 molecules CL

#9: Protein 30S ribosomal protein S3


Mass: 26031.316 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7V3
#18: Protein 30S ribosomal protein S12 / Small ribosomal subunit protein uS12


Mass: 13814.249 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsL, strA, b3342, JW3304 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7S3

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Non-polymers , 4 types, 289 molecules

#54: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#55: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 272 / Source method: obtained synthetically / Formula: Mg
#56: Chemical
ChemComp-SPD / SPERMIDINE / N-(2-AMINO-PROPYL)-1,4-DIAMINOBUTANE / PA(34)


Mass: 145.246 Da / Num. of mol.: 14 / Source method: obtained synthetically / Formula: C7H19N3
#57: Chemical ChemComp-SPM / SPERMINE


Mass: 202.340 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H26N4

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Details

Has ligand of interestN
Has protein modificationY

-
Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

-
Sample preparation

ComponentName: Chimeric Escherichia coli 70S ribosome containing an evolved Vibrio cholerae 16S rRNA (VC-S4.4)
Type: RIBOSOME / Entity ID: #1-#53 / Source: RECOMBINANT
Source (natural)Organism: Escherichia coli (E. coli)
Source (recombinant)Organism: Escherichia coli (E. coli)
Buffer solutionpH: 7.4
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

-
Electron microscopy imaging

MicroscopyModel: TFS GLACIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 500 nm
Image recordingElectron dose: 60 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k)

-
Processing

EM software
IDNameCategory
1cisTEMparticle selection
13cisTEM3D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 450287 / Symmetry type: POINT

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