National Institutes of Health/National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIH/NIAMS)
United States
Citation
Journal: J Biol Chem / Year: 2026 Title: Structural Basis for HIV-1 Rev Recognition by the Histone Chaperone Human Nap1. Authors: Elif Eren / Norman R Watts / Dennis C Winkler / Paul T Wingfield / Abstract: Human Nap1 (hNap1) is a histone chaperone involved in chromatin dynamics and has been shown to interact with the HIV-1 regulatory protein Rev, which is essential for nuclear export of viral RNA. ...Human Nap1 (hNap1) is a histone chaperone involved in chromatin dynamics and has been shown to interact with the HIV-1 regulatory protein Rev, which is essential for nuclear export of viral RNA. Despite the functional significance of this interaction, its structural basis has remained elusive. Here, we present the X-ray crystal structure of hNap1 and the cryo-electron microscopy structure of the core domain of hNap1-Rev complex. The structure reveals that hNap1 binds Rev dimers via its acidic concave surface, engaging the Rev arginine-rich motif and oligomerization domain, and stabilizes Rev as a dimer-of-dimers tetramer. This interaction prevents higher-order Rev aggregation and enhances Rev's cooperative binding to the Rev Response Element. Surface plasmon resonance measurements confirm the formation of a stable complex with an apparent low-micromolar affinity between hNap1 and Rev, supporting a chaperone-like, reversible association. Our findings provide molecular insight into how hNap1 modulates Rev assembly and function, suggesting a model in which hNap1 primes Rev for productive engagement with viral RNA, thereby facilitating HIV-1 replication.
A1: Protein Rev B1: Protein Rev C1: Protein Rev D1: Protein Rev E1: Protein Rev F1: Protein Rev G1: Protein Rev H1: Protein Rev I1: Protein Rev J1: Protein Rev K1: Protein Rev L1: Protein Rev M1: Protein Rev N1: Protein Rev O1: Protein Rev P1: Protein Rev R1: Protein Rev S1: Protein Rev T1: Protein Rev U1: Protein Rev V1: Protein Rev W1: Protein Rev X1: Protein Rev Y1: Protein Rev c2: Protein Rev d2: Protein Rev e2: Protein Rev f2: Protein Rev g2: Protein Rev h2: Protein Rev v2: Protein Rev w2: Protein Rev x2: Protein Rev y2: Protein Rev a3: Protein Rev b3: Protein Rev i3: Protein Rev j3: Protein Rev k3: Protein Rev l3: Protein Rev m3: Protein Rev n3: Protein Rev o3: Protein Rev p3: Protein Rev r3: Protein Rev s3: Protein Rev t3: Protein Rev u3: Protein Rev A4: Protein Rev B4: Protein Rev I4: Protein Rev J4: Protein Rev K4: Protein Rev L4: Protein Rev M4: Protein Rev N4: Protein Rev O4: Protein Rev R4: Protein Rev S4: Protein Rev T4: Protein Rev U4: Protein Rev A5: Protein Rev B5: Protein Rev C5: Protein Rev D5: Protein Rev E5: Protein Rev F5: Protein Rev G5: Protein Rev H5: Protein Rev M5: Protein Rev N5: Protein Rev O5: Protein Rev P5: Protein Rev T5: Protein Rev U5: Protein Rev V5: Protein Rev W5: Protein Rev X5: Protein Rev Y5: Protein Rev C6: Protein Rev D6: Protein Rev E6: Protein Rev F6: Protein Rev G6: Protein Rev H6: Protein Rev V6: Protein Rev W6: Protein Rev X6: Protein Rev Y6: Protein Rev a7: Protein Rev b7: Protein Rev c7: Protein Rev d7: Protein Rev e7: Protein Rev f7: Protein Rev g7: Protein Rev h7: Protein Rev i7: Protein Rev j7: Protein Rev k7: Protein Rev l7: Protein Rev m7: Protein Rev n7: Protein Rev o7: Protein Rev p7: Protein Rev r7: Protein Rev s7: Protein Rev t7: Protein Rev u7: Protein Rev v7: Protein Rev w7: Protein Rev x7: Protein Rev y7: Protein Rev a8: Protein Rev b8: Protein Rev c8: Protein Rev d8: Protein Rev e8: Protein Rev f8: Protein Rev g8: Protein Rev h8: Protein Rev m8: Protein Rev n8: Protein Rev o8: Protein Rev p8: Protein Rev t8: Protein Rev u8: Protein Rev v8: Protein Rev w8: Protein Rev x8: Protein Rev y8: Protein Rev A9: Protein Rev B9: Protein Rev C9: Protein Rev D9: Protein Rev E9: Protein Rev F9: Protein Rev G9: Protein Rev H9: Protein Rev I9: Protein Rev J9: Protein Rev K9: Protein Rev L9: Protein Rev M9: Protein Rev N9: Protein Rev O9: Protein Rev P9: Protein Rev R9: Protein Rev S9: Protein Rev T9: Protein Rev U9: Protein Rev V9: Protein Rev W9: Protein Rev X9: Protein Rev Y9: Protein Rev
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Human immunodeficiency virus 1
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United States, 1items 
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Escherichia coli (E. coli) / References: UniProt: Q76PP8
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FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
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