+
データを開く
-
基本情報
登録情報 | データベース: EMDB / ID: EMD-9957 | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
タイトル | C2S2M2N2-type PSII-LHCII | ||||||||||||
![]() | |||||||||||||
![]() |
| ||||||||||||
![]() | Photosystem II / C2S2M2N2-type PSII-LHCII / PHOTOSYNTHESIS | ||||||||||||
機能・相同性 | ![]() thylakoid light-harvesting complex / photosystem II antenna complex / PSII associated light-harvesting complex II / nonphotochemical quenching / photosynthesis, light harvesting in photosystem I / photosynthesis, light harvesting / photosystem II oxygen evolving complex / photosystem II assembly / oxygen evolving activity / photosystem II stabilization ...thylakoid light-harvesting complex / photosystem II antenna complex / PSII associated light-harvesting complex II / nonphotochemical quenching / photosynthesis, light harvesting in photosystem I / photosynthesis, light harvesting / photosystem II oxygen evolving complex / photosystem II assembly / oxygen evolving activity / photosystem II stabilization / photosystem II reaction center / photosystem II / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / photosystem I / response to herbicide / photosystem II / chlorophyll binding / photosynthetic electron transport in photosystem II / phosphate ion binding / photosynthesis, light reaction / chloroplast thylakoid membrane / : / response to light stimulus / photosynthesis / electron transfer activity / protein stabilization / iron ion binding / protein domain specific binding / mRNA binding / heme binding / metal ion binding 類似検索 - 分子機能 | ||||||||||||
生物種 | ![]() ![]() | ||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.73 Å | ||||||||||||
![]() | Chang SH / Shen LL | ||||||||||||
資金援助 | ![]()
| ||||||||||||
![]() | ![]() タイトル: Structure of a CSMN-type PSII-LHCII supercomplex from the green alga . 著者: Liangliang Shen / Zihui Huang / Shenghai Chang / Wenda Wang / Jingfen Wang / Tingyun Kuang / Guangye Han / Jian-Ren Shen / Xing Zhang / ![]() ![]() 要旨: Photosystem II (PSII) in the thylakoid membranes of plants, algae, and cyanobacteria catalyzes light-induced oxidation of water by which light energy is converted to chemical energy and molecular ...Photosystem II (PSII) in the thylakoid membranes of plants, algae, and cyanobacteria catalyzes light-induced oxidation of water by which light energy is converted to chemical energy and molecular oxygen is produced. In higher plants and most eukaryotic algae, the PSII core is surrounded by variable numbers of light-harvesting antenna complex II (LHCII), forming a PSII-LHCII supercomplex. In order to harvest energy efficiently at low-light-intensity conditions under water, a complete PSII-LHCII supercomplex (CSMN) of the green alga (Cr) contains more antenna subunits and pigments than the dominant PSII-LHCII supercomplex (CSM) of plants. The detailed structure and energy transfer pathway of the Cr-PSII-LHCII remain unknown. Here we report a cryoelectron microscopy structure of a complete, CSMN-type PSII-LHCII supercomplex from at 3.37-Å resolution. The results show that the Cr-CSMN supercomplex is organized as a dimer, with 3 LHCII trimers, 1 CP26, and 1 CP29 peripheral antenna subunits surrounding each PSII core. The N-LHCII trimer partially occupies the position of CP24, which is present in the higher-plant PSII-LHCII but absent in the green alga. The M trimer is rotated relative to the corresponding M trimer in plant PSII-LHCII. In addition, some unique features were found in the green algal PSII core. The arrangement of a huge number of pigments allowed us to deduce possible energy transfer pathways from the peripheral antennae to the PSII core. | ||||||||||||
履歴 |
|
-
構造の表示
ムービー |
![]() |
---|---|
構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
-
ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 95.5 MB | ![]() | |
---|---|---|---|---|
ヘッダ (付随情報) | ![]() ![]() | 43.3 KB 43.3 KB | 表示 表示 | ![]() |
画像 | ![]() | 79 KB | ||
Filedesc metadata | ![]() | 9.8 KB | ||
その他 | ![]() ![]() | 74.3 MB 73 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
-
リンク
EMDBのページ | ![]() ![]() |
---|---|
「今月の分子」の関連する項目 |
-
マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.307 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
|
-添付データ
-追加マップ: #2
ファイル | emd_9957_additional_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
投影像・断面図 |
| ||||||||||||
密度ヒストグラム |
-追加マップ: #1
ファイル | emd_9957_additional_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
投影像・断面図 |
| ||||||||||||
密度ヒストグラム |
-
試料の構成要素
+全体 : C2S2M2N2-type PSII-LHCII supercomplex of Chlamydomonas reinhardtii
+超分子 #1: C2S2M2N2-type PSII-LHCII supercomplex of Chlamydomonas reinhardtii
+分子 #1: Chlorophyll a-b binding protein CP29
+分子 #2: Photosystem II protein D1
+分子 #3: Photosystem II D2 protein
+分子 #4: Cytochrome b559 subunit beta
+分子 #5: Photosystem II reaction center protein H
+分子 #6: Photosystem II reaction center protein I
+分子 #7: Photosystem II reaction center protein J, PsbJ
+分子 #8: Photosystem II reaction center protein K
+分子 #9: Photosystem II reaction center protein L
+分子 #10: Photosystem II reaction center protein M
+分子 #11: Oxygen-evolving enhancer protein 1 of photosystem II
+分子 #12: Photosystem II reaction center protein T
+分子 #13: Photosystem II reaction center W protein, chloroplastic
+分子 #14: 4.1 kDa photosystem II subunit
+分子 #15: Photosystem II reaction center protein Z
+分子 #16: Photosystem II reaction center protein 30, Psb30
+分子 #17: Cytochrome b559 subunit alpha
+分子 #18: Chlorophyll a-b binding protein, chloroplastic
+分子 #19: Chlorophyll a-b binding protein, chloroplastic
+分子 #20: Photosystem II CP43 reaction center protein
+分子 #21: Photosystem II CP47 reaction center protein
+分子 #22: CHLOROPHYLL A
+分子 #23: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
+分子 #24: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...
+分子 #25: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+分子 #26: CHLOROPHYLL B
+分子 #27: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY...
+分子 #28: FE (II) ION
+分子 #29: CHLORIDE ION
+分子 #30: PHEOPHYTIN A
+分子 #31: BETA-CAROTENE
+分子 #32: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+分子 #33: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...
+分子 #34: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+分子 #35: BICARBONATE ION
+分子 #36: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+分子 #37: PROTOPORPHYRIN IX CONTAINING FE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
---|---|
![]() | 単粒子再構成法 |
試料の集合状態 | particle |
-
試料調製
緩衝液 | pH: 7.5 |
---|---|
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % |
-
電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
---|---|
撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 検出モード: SUPER-RESOLUTION / 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: OTHER / 撮影モード: BRIGHT FIELD |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
-
画像解析
初期モデル | モデルのタイプ: OTHER |
---|---|
最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 3.73 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 89018 |
初期 角度割当 | タイプ: MAXIMUM LIKELIHOOD |
最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD |