|Entry||Database: EMDB / ID: EMD-9766|
|Title||Doublet microtubule of fap45 null mutant|
|Sample||axoneme of Chlamydomonas reinhardtii|
|Biological species||Chlamydomonas reinhardtii (plant)|
|Method||subtomogram averaging / cryo EM / Resolution: 39.6 Å|
|Authors||Owa M / Uchihashi T / Yanagisawa H / Yamano T / Iguchi H / Fukuzawa H / Wakabayashi K / Ando T / Kikkawa M|
|Citation||Journal: Nat Commun / Year: 2019|
Title: Inner lumen proteins stabilize doublet microtubules in cilia and flagella.
Authors: Mikito Owa / Takayuki Uchihashi / Haru-Aki Yanagisawa / Takashi Yamano / Hiro Iguchi / Hideya Fukuzawa / Ken-Ichi Wakabayashi / Toshio Ando / Masahide Kikkawa /
Abstract: Motile cilia are microtubule-based organelles that play important roles in most eukaryotes. Although axonemal microtubules are sufficiently stable to withstand their beating motion, it remains ...Motile cilia are microtubule-based organelles that play important roles in most eukaryotes. Although axonemal microtubules are sufficiently stable to withstand their beating motion, it remains unknown how they are stabilized while serving as tracks for axonemal dyneins. To address this question, we have identified two uncharacterized proteins, FAP45 and FAP52, as microtubule inner proteins (MIPs) in Chlamydomonas. These proteins are conserved among eukaryotes with motile cilia. Using cryo-electron tomography (cryo-ET) and high-speed atomic force microscopy (HS-AFM), we show that lack of these proteins leads to a loss of inner protrusions in B-tubules and less stable microtubules. These protrusions are located near the inner junctions of doublet microtubules and lack of both FAP52 and a known inner junction protein FAP20 results in detachment of the B-tubule from the A-tubule, as well as flagellar shortening. These results demonstrate that FAP45 and FAP52 bind to the inside of microtubules and stabilize ciliary axonemes.
|Structure viewer||EM map: |
Downloads & links
|File||Download / File: emd_9766.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 7.1 Å|
|Symmetry||Space group: 1|
CCP4 map header:
-Entire axoneme of Chlamydomonas reinhardtii
|Entire||Name: axoneme of Chlamydomonas reinhardtii / Number of components: 1|
-Component #1: cellular-component, axoneme of Chlamydomonas reinhardtii
|Cellular-component||Name: axoneme of Chlamydomonas reinhardtii / Recombinant expression: No|
|Source||Species: Chlamydomonas reinhardtii (plant) / Strain: fap45|
|Specimen||Specimen state: Filament / Method: cryo EM|
|Sample solution||Specimen conc.: 0.02 mg/mL / pH: 7.4|
|Vitrification||Cryogen name: ETHANE|
-Electron microscopy imaging
|Imaging||Microscope: JEOL 3100FFC|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 100 e/Å2 / Illumination mode: OTHER|
|Lens||Imaging mode: DIFFRACTION|
|Specimen Holder||Model: OTHER|
|Camera||Detector: GATAN K2 SUMMIT (4k x 4k)|
|Processing||Method: subtomogram averaging / Applied symmetry: C1 (asymmetric) / Number of subtomograms: 2200|
|3D reconstruction||Resolution: 39.6 Å / Resolution method: FSC 0.5 CUT-OFF|
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