|Entry||Database: EMDB / ID: 8833|
|Map data||Model of BRCA1-BARD1 isolated from the mutated human breast cancer cell line HCC1937|
|Sample||Mutated BRCA1-BARD1 complex.:|
|Function / homology||Ankyrin repeat region circular profile. / Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) / Homologous DNA Pairing and Strand Exchange / BRCT domain superfamily / Nonhomologous End-Joining (NHEJ) / BRCA1-associated RING domain protein 1 / BRCA1-associated / BRCA1, serine-rich domain / Resolution of D-loop Structures through Holliday Junction Intermediates / BRCT domain ...Ankyrin repeat region circular profile. / Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) / Homologous DNA Pairing and Strand Exchange / BRCT domain superfamily / Nonhomologous End-Joining (NHEJ) / BRCA1-associated RING domain protein 1 / BRCA1-associated / BRCA1, serine-rich domain / Resolution of D-loop Structures through Holliday Junction Intermediates / BRCT domain / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / Zinc finger, C3HC4 RING-type / Zinc finger, RING-type, conserved site / Zinc finger, C3HC4 type (RING finger) / Meiotic synapsis / Metalloprotease DUBs / Zinc finger, RING/FYVE/PHD-type / Breast cancer type 1 susceptibility protein (BRCA1) / Ankyrin repeat / UCH proteinases / HDR through Homologous Recombination (HRR) / HDR through Single Strand Annealing (SSA) / SUMOylation of DNA damage response and repair proteins / Zinc finger, RING-type / Ankyrin repeat-containing domain superfamily / Ankyrin repeat-containing domain / Processing of DNA double-strand break ends / Serine-rich domain associated with BRCT / Meiotic recombination / BRCT domain profile. / Transcriptional Regulation by E2F6 / Ankyrin repeat profile. / G2/M DNA damage checkpoint / Regulation of TP53 Activity through Phosphorylation / Zinc finger RING-type signature. / TP53 Regulates Transcription of DNA Repair Genes / Presynaptic phase of homologous DNA pairing and strand exchange / Zinc finger RING-type profile. / BRCA1 C Terminus (BRCT) domain / Ankyrin repeats (3 copies) / negative regulation of mRNA 3'-end processing / positive regulation of histone H4-K16 acetylation / mitotic G2/M transition checkpoint / positive regulation of histone H4-K20 methylation / gamma-tubulin ring complex / BRCA1-BARD1 complex / BRCA1-A complex / dosage compensation by inactivation of X chromosome / regulation of transcription by RNA polymerase III / negative regulation of histone H3-K9 methylation / negative regulation of centriole replication / positive regulation of histone H3-K9 methylation / negative regulation of histone H3-K4 methylation / negative regulation of intracellular estrogen receptor signaling pathway / protein K6-linked ubiquitination / lateral element / positive regulation of histone H3-K9 acetylation / negative regulation of histone acetylation / chordate embryonic development / regulation of phosphorylation / cellular response to indole-3-methanol / negative regulation of fatty acid biosynthetic process / DNA double-strand break processing / regulation of gene expression by genetic imprinting / regulation of DNA methylation / RNA polymerase binding / postreplication repair / centrosome cycle / positive regulation of histone H3-K4 methylation / positive regulation of histone acetylation / tissue homeostasis / response to ionizing radiation / negative regulation of protein export from nucleus / negative regulation of reactive oxygen species metabolic process / signal transduction involved in G2 DNA damage checkpoint / positive regulation of vascular endothelial growth factor production / negative regulation of G0 to G1 transition / positive regulation of DNA repair / protein autoubiquitination / ubiquitin ligase complex / DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / tubulin binding / go:0001105: / cytoplasmic ribonucleoprotein granule / positive regulation of protein ubiquitination / double-strand break repair / androgen receptor binding / androgen receptor signaling pathway / positive regulation of cell cycle arrest / chromosome segregation / negative regulation of extrinsic apoptotic signaling pathway via death domain receptors / double-strand break repair via nonhomologous end joining / ribonucleoprotein complex / double-strand break repair via homologous recombination / positive regulation of protein catabolic process / kinase binding / ec:126.96.36.199: / fatty acid biosynthetic process / cell cycle arrest / chromosome|
Function and homology information
|Source||Homo sapiens (human)|
|Method||single particle reconstruction / negative staining / 14.7 Å resolution|
|Authors||Kelly DF / Dearnaley WJ|
|Citation||Journal: Sci Adv / Year: 2017|
Title: Structural analysis of BRCA1 reveals modification hotspot.
Authors: Yanping Liang / William J Dearnaley / A Cameron Varano / Carly E Winton / Brian L Gilmore / Nick A Alden / Zhi Sheng / Deborah F Kelly
|Date||Deposition: Jul 17, 2017 / Header (metadata) release: Aug 9, 2017 / Map release: Oct 11, 2017 / Last update: Feb 14, 2018|
|Structure viewer||EM map: |
Downloads & links
|File||emd_8833.map.gz (map file in CCP4 format, 865 KB)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 4.4 Å|
CCP4 map header:
-Entire Mutated BRCA1-BARD1 complex.
|Entire||Name: Mutated BRCA1-BARD1 complex. / Number of components: 1|
-Component #1: protein, Mutated BRCA1-BARD1 complex.
|Protein||Name: Mutated BRCA1-BARD1 complex. / Recombinant expression: No|
|Source||Species: Homo sapiens (human)|
|Specimen||Specimen state: particle / Method: negative staining|
|Sample solution||Specimen conc.: 0.02 mg/ml|
Buffer solution: 20 mM HEPES buffer pH 7.2, 150 mM NaCl, 10 mM CaCl2, 10 mM MgCl2
|Staining||1% uranyl formate|
|Vitrification||Cryogen name: NONE|
-Electron microscopy imaging
Model: Tecnai Spirit / Image courtesy: FEI Company
|Imaging||Microscope: FEI TECNAI SPIRIT|
|Electron gun||Electron source: LAB6 / Accelerating voltage: 120 kV / Electron dose: 5 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Magnification: 68000 X (nominal) / Cs: 2 mm / Imaging mode: BRIGHT FIELD / Defocus: -1500 - nm|
|Specimen Holder||Model: SIDE ENTRY, EUCENTRIC|
|Camera||Detector: FEI EAGLE (2k x 2k)|
|Image acquisition||Number of digital images: 100 / Sampling size: 30 microns|
|Processing||Method: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 4222|
|3D reconstruction||Software: RELION / Resolution: 14.7 Å / Resolution method: FSC 0.5 CUT-OFF / Euler angles: Empirical Bayesian approach|
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