- EMDB-8401: Single particle cryo-EM reconstruction of the Salmonella SPI-1 ty... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-8401
タイトル
Single particle cryo-EM reconstruction of the Salmonella SPI-1 type III secretion injectisome secretin InvG at 4.4 Angstrom resolution
マップデータ
Single particle cryo-EM reconstruction of the Salmonella SPI-1 type III secretion injectisome secretin InvG at 4.4 Angstrom resolution
試料
複合体: Type III injectisome basal body
タンパク質・ペプチド: InvG
機能・相同性
機能・相同性情報
type III protein secretion system complex / type II protein secretion system complex / protein secretion by the type III secretion system / protein secretion / cell outer membrane / identical protein binding 類似検索 - 分子機能
: / SPI-1 type 3 secretion system secretin, N0 domain / Type III secretion system outer membrane pore YscC/HrcC / Bacterial type II secretion system protein D signature. / Type II secretion system protein GspD, conserved site / NolW-like / Bacterial type II/III secretion system short domain / NolW-like superfamily / Type II/III secretion system / Bacterial type II and III secretion system protein 類似検索 - ドメイン・相同性
SPI-1 type 3 secretion system secretin 類似検索 - 構成要素
ジャーナル: Nature / 年: 2016 タイトル: Near-atomic-resolution cryo-EM analysis of the Salmonella T3S injectisome basal body. 著者: L J Worrall / C Hong / M Vuckovic / W Deng / J R C Bergeron / D D Majewski / R K Huang / T Spreter / B B Finlay / Z Yu / N C J Strynadka / 要旨: The type III secretion (T3S) injectisome is a specialized protein nanomachine that is critical for the pathogenicity of many Gram-negative bacteria, including purveyors of plague, typhoid fever, ...The type III secretion (T3S) injectisome is a specialized protein nanomachine that is critical for the pathogenicity of many Gram-negative bacteria, including purveyors of plague, typhoid fever, whooping cough, sexually transmitted infections and major nosocomial infections. This syringe-shaped 3.5-MDa macromolecular assembly spans both bacterial membranes and that of the infected host cell. The internal channel formed by the injectisome allows for the direct delivery of partially unfolded virulence effectors into the host cytoplasm. The structural foundation of the injectisome is the basal body, a molecular lock-nut structure composed predominantly of three proteins that form highly oligomerized concentric rings spanning the inner and outer membranes. Here we present the structure of the prototypical Salmonella enterica serovar Typhimurium pathogenicity island 1 basal body, determined using single-particle cryo-electron microscopy, with the inner-membrane-ring and outer-membrane-ring oligomers defined at 4.3 Å and 3.6 Å resolution, respectively. This work presents the first, to our knowledge, high-resolution structural characterization of the major components of the basal body in the assembled state, including that of the widespread class of outer-membrane portals known as secretins.