European Molecular Biology Organization (EMBO) Short-term fellowship
ASTF 352-2015
Canadian Institutes of Health Research (CIHR)
カナダ
European Molecular Biology Organization (EMBO) Long-term fellowship
ALTF 51-2014
Wellcome Trust
097383
英国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
PHS GM103314 and PHS GM109824
米国
Biotechnology and Biological Sciences Research Council (BBSRC)
BB/F010656/1
英国
FEBS long-term fellowship
Robertson Foundation
米国
Fonds de Recherche du Quebec-Sante (FRQ-S)
カナダ
引用
ジャーナル: Nat Commun / 年: 2016 タイトル: UtpA and UtpB chaperone nascent pre-ribosomal RNA and U3 snoRNA to initiate eukaryotic ribosome assembly. 著者: Mirjam Hunziker / Jonas Barandun / Elisabeth Petfalski / Dongyan Tan / Clémentine Delan-Forino / Kelly R Molloy / Kelly H Kim / Hywel Dunn-Davies / Yi Shi / Malik Chaker-Margot / Brian T ...著者: Mirjam Hunziker / Jonas Barandun / Elisabeth Petfalski / Dongyan Tan / Clémentine Delan-Forino / Kelly R Molloy / Kelly H Kim / Hywel Dunn-Davies / Yi Shi / Malik Chaker-Margot / Brian T Chait / Thomas Walz / David Tollervey / Sebastian Klinge / 要旨: Early eukaryotic ribosome biogenesis involves large multi-protein complexes, which co-transcriptionally associate with pre-ribosomal RNA to form the small subunit processome. The precise mechanisms ...Early eukaryotic ribosome biogenesis involves large multi-protein complexes, which co-transcriptionally associate with pre-ribosomal RNA to form the small subunit processome. The precise mechanisms by which two of the largest multi-protein complexes-UtpA and UtpB-interact with nascent pre-ribosomal RNA are poorly understood. Here, we combined biochemical and structural biology approaches with ensembles of RNA-protein cross-linking data to elucidate the essential functions of both complexes. We show that UtpA contains a large composite RNA-binding site and captures the 5' end of pre-ribosomal RNA. UtpB forms an extended structure that binds early pre-ribosomal intermediates in close proximity to architectural sites such as an RNA duplex formed by the 5' ETS and U3 snoRNA as well as the 3' boundary of the 18S rRNA. Both complexes therefore act as vital RNA chaperones to initiate eukaryotic ribosome assembly.