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Yorodumi- EMDB-79007: LPT translocon complex from Flavobacter johnsoniae built in compo... -
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Open data
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Basic information
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| Title | LPT translocon complex from Flavobacter johnsoniae built in composite volume | ||||||||||||
Map data | composite volume | ||||||||||||
Sample |
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Keywords | LPS transport SLP transport outer membrane protein complex / MEMBRANE PROTEIN | ||||||||||||
| Function / homology | Function and homology informationglycolipid transfer activity / transporter complex / outer membrane / lipopolysaccharide transport / Gram-negative-bacterium-type cell outer membrane assembly / cell outer membrane / outer membrane-bounded periplasmic space Similarity search - Function | ||||||||||||
| Biological species | Flavobacterium johnsoniae UW101 (bacteria) | ||||||||||||
| Method | single particle reconstruction / cryo EM / Resolution: 3.2 Å | ||||||||||||
Authors | Lea SM / Berks BC | ||||||||||||
| Funding support | United States, European Union, 3 items
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Citation | Journal: To Be PublishedTitle: An expanded LPS translocon mediates surface lipoprotein export in the Bacteroidota Authors: Liu X / Orenday Tapia L / Bryant OJ / Lea SM / Berks BC | ||||||||||||
| History |
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Structure visualization
| Supplemental images |
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Downloads & links
-EMDB archive
| Map data | emd_79007.map.gz | 186.3 MB | EMDB map data format | |
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| Header (meta data) | emd-79007-v30.xml emd-79007.xml | 23 KB 23 KB | Display Display | EMDB header |
| Images | emd_79007.png | 33.9 KB | ||
| Filedesc metadata | emd-79007.cif.gz | 8.5 KB | ||
| Archive directory | https://data.pdbj.org/pub/emdb/structures/EMD-79007 ftp://data.pdbj.org/pub/emdb/structures/EMD-79007 | HTTPS FTP |
-Related structure data
| Related structure data | ![]() 38olMC ![]() 38qpC C: citing same article ( M: atomic model generated by this map |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
| EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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| Related items in Molecule of the Month |
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Map
| File | Download / File: emd_79007.map.gz / Format: CCP4 / Size: 209.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| Annotation | composite volume | ||||||||||||||||||||||||||||||||||||
| Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
| Voxel size | X=Y=Z: 1.315 Å | ||||||||||||||||||||||||||||||||||||
| Density |
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| Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
| Details | EMDB XML:
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-Supplemental data
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Sample components
+Entire : LPT complex
+Supramolecule #1: LPT complex
+Macromolecule #1: Hypothetical lipoprotein
+Macromolecule #2: OstA family protein
+Macromolecule #3: Porin
+Macromolecule #4: Hypothetical lipoprotein
+Macromolecule #5: Outer membrane protein beta-barrel domain-containing protein
+Macromolecule #6: Hypothetical lipoprotein
+Macromolecule #7: DUF5683 domain-containing protein
+Macromolecule #8: Organic solvent tolerance protein OstA-like protein
+Macromolecule #9: Hypothetical lipoprotein
+Macromolecule #12: alpha-D-galactopyranose
-Experimental details
-Structure determination
| Method | cryo EM |
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Processing | single particle reconstruction |
| Aggregation state | particle |
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Sample preparation
| Buffer | pH: 7.4 |
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| Vitrification | Cryogen name: ETHANE |
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Electron microscopy
| Microscope | TFS KRIOS |
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| Details | Data were collected at both 200kV and 300kV on two different Titan Krios and an Arctica |
| Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2 Details: Data were collected on three microscopes Titan Krios / Gatan K3 BioQuantum Titan Krios / Falcon 4i Arctica / Gatan K3 BioQuantum |
| Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
| Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm |
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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About Yorodumi



Keywords
Flavobacterium johnsoniae UW101 (bacteria)
Authors
United States, European Union, 3 items
Citation













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Processing
FIELD EMISSION GUN
