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- EMDB-77157: VIPR Binary Complex with two filaments -

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Basic information

Entry
Database: EMDB / ID: EMD-77157
TitleVIPR Binary Complex with two filaments
Map dataSharpened Map
Sample
  • Complex: Binary complex of the SUSP1 Vipr protein with an associated viprRNA.
    • Protein or peptide: Vipr Protein
    • RNA: vrRNA
KeywordsRibonucleoprotein / VIPR / RNA Binding Protein / Binary Complex / RNA BINDING PROTEIN-RNA complex
Biological speciesSuspvirus SUSP1
Methodsingle particle reconstruction / cryo EM / Resolution: 2.7 Å
AuthorsDocter TA / Yoon PH / Zhang Z / Brohawn SG / Doudna JA
Funding support United States, 1 items
OrganizationGrant numberCountry
National Science Foundation (NSF, United States)NSF 2334028 United States
CitationJournal: Science / Year: 2026
Title: VIPR RNA-guided DNA recognition by noncontiguous geometric triplex formation
Authors: Yoon PH / Docter TA / Zhang ZT / Loi K / Lopez SC / Valentin-Alvarado LE / Tuck O / Brohawn SG / Doudna JA
History
DepositionMay 14, 2026-
Header (metadata) releaseSep 16, 2026-
Map releaseSep 16, 2026-
UpdateSep 16, 2026-
Current statusSep 16, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_77157.png

Downloads & links

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Map

FileDownload / File: emd_77157.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationSharpened Map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.85 Å/pix.
x 600 pix.
= 508.8 Å		0.85 Å/pix.
x 600 pix.
= 508.8 Å		0.85 Å/pix.
x 600 pix.
= 508.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.848 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.04
Minimum - Maximum-0.36526176 - 0.6167038
Average (Standard dev.)-0.000018911884 (±0.006265453)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions600600600
Spacing600600600
CellA=B=C: 508.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Unsharpened Map

Fileemd_77157_additional_1.map
AnnotationUnsharpened Map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half Map A

Fileemd_77157_half_map_1.map
AnnotationHalf Map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half Map B

Fileemd_77157_half_map_2.map
AnnotationHalf Map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Binary complex of the SUSP1 Vipr protein with an associated viprRNA.

EntireName: Binary complex of the SUSP1 Vipr protein with an associated viprRNA.
Components
  • Complex: Binary complex of the SUSP1 Vipr protein with an associated viprRNA.
    • Protein or peptide: Vipr Protein
    • RNA: vrRNA

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Supramolecule #1: Binary complex of the SUSP1 Vipr protein with an associated viprRNA.

SupramoleculeName: Binary complex of the SUSP1 Vipr protein with an associated viprRNA.
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Details: Dimeric conformation of the SUSP1 Vipr Binary complex with an associated viprRNA.
Source (natural)Organism: Suspvirus SUSP1

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Macromolecule #1: Vipr Protein

MacromoleculeName: Vipr Protein / type: protein_or_peptide / ID: 1 / Number of copies: 7 / Enantiomer: LEVO
Source (natural)Organism: Suspvirus SUSP1
Molecular weightTheoretical: 22.979531 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MKLLNIKITG TRPFLSHADT LSDPLNPLTK YHKSLSSKRK KTDEDYALLA ESQLVTSCYY DEQLGFVMNG EMIEACIKSG AKLNKLGKV IDRAIMLTDV VFPMTIKNCP ANPQELAKNQ DFIYAKSVKI GTARVMSYRP IFRDWSVEFG LMFDEEQITK E ELLMVLEN ...String:
MKLLNIKITG TRPFLSHADT LSDPLNPLTK YHKSLSSKRK KTDEDYALLA ESQLVTSCYY DEQLGFVMNG EMIEACIKSG AKLNKLGKV IDRAIMLTDV VFPMTIKNCP ANPQELAKNQ DFIYAKSVKI GTARVMSYRP IFRDWSVEFG LMFDEEQITK E ELLMVLEN AGNLCGVGDW RPRFGRFSVE IMSEGNVENL YFQS

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Macromolecule #2: vrRNA

MacromoleculeName: vrRNA / type: rna / ID: 2
Details: Resolved segment of a SUSP1 viprRNA. We note that the structure of the 3' pseudoknot was confirmed in additional structures present within our publication and allowed for a more ...Details: Resolved segment of a SUSP1 viprRNA. We note that the structure of the 3' pseudoknot was confirmed in additional structures present within our publication and allowed for a more comprehensive model. We did not use any additional maps for the refinement of the Pseudoknot.
Number of copies: 1
Source (natural)Organism: Suspvirus SUSP1
Molecular weightTheoretical: 30.630064 KDa
SequenceString:
UAUGGUAAGG UGUGGUUCGG UAAGGUGCGG UGGGGUAAGG CUAGGUUUGG UCUGGUGUGG GUGGAUGGUA GUAGCCACGU UAAAAAUCU ACCAA

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.2 µm / Nominal defocus min: 0.6 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 212926
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE

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