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- EMDB-75794: Bacteriophage Goslar Collar -

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ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-75794
TitleBacteriophage Goslar Collar
Map data
Sample
  • Virus: Goslarvirus
    • Protein or peptide: Whiskers Protein gp126
    • Protein or peptide: Tail Tube of Bacteriophage Goslar
    • Protein or peptide: Tail sheath protein gp29 gp29PR domain-containing protein
    • Protein or peptide: Tail Adaptor Protein gp50 NTD
    • Protein or peptide: Tail Terminator protein gp51
KeywordsCollar / WAC / Whiskers / VIRAL PROTEIN
Function / homology
Function and homology information


: / : / Putative phage head-tail joining protein / Phage head to tail associated domain / : / Phage tail tube protein / : / Bacteriophage phiKZ, gp29PR
Similarity search - Domain/homology
Uncharacterized protein / Virion structural protein / Tail sheath protein gp29 gp29PR domain-containing protein
Similarity search - Component
Biological speciesGoslarvirus
Methodsingle particle reconstruction / cryo EM / Resolution: 4.16 Å
AuthorsBasu D / Gu Y / Corbett KD
Funding support United States, 1 items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI)Emerging Pathogens Initiative United States
CitationJournal: To Be Published
Title: Bacteriophage Goslar Collar
Authors: Basu D
History
DepositionMar 2, 2026-
Header (metadata) releaseAug 19, 2026-
Map releaseAug 19, 2026-
UpdateAug 19, 2026-
Current statusAug 19, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75794.png

Downloads & links

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Map

FileDownload / File: emd_75794.map.gz / Format: CCP4 / Size: 1000 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.84 Å/pix.
x 640 pix.
= 1176. Å		1.84 Å/pix.
x 640 pix.
= 1176. Å		1.84 Å/pix.
x 640 pix.
= 1176. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.8375 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.09
Minimum - Maximum-0.5452262 - 1.1243379
Average (Standard dev.)-0.0011725317 (±0.057307955)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions640640640
Spacing640640640
CellA=B=C: 1176.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_75794_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_75794_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Goslarvirus

EntireName: Goslarvirus
Components
  • Virus: Goslarvirus
    • Protein or peptide: Whiskers Protein gp126
    • Protein or peptide: Tail Tube of Bacteriophage Goslar
    • Protein or peptide: Tail sheath protein gp29 gp29PR domain-containing protein
    • Protein or peptide: Tail Adaptor Protein gp50 NTD
    • Protein or peptide: Tail Terminator protein gp51

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Supramolecule #1: Goslarvirus

SupramoleculeName: Goslarvirus / type: virus / ID: 1 / Parent: 0 / Macromolecule list: #5, #1-#4 / NCBI-ID: 2733105 / Sci species name: Goslarvirus / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No
Host (natural)Organism: Escherichia coli (E. coli)

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Macromolecule #1: Tail Tube of Bacteriophage Goslar

MacromoleculeName: Tail Tube of Bacteriophage Goslar / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Goslarvirus
Molecular weightTheoretical: 32.990105 KDa
SequenceString: MRPDNTLLGE QGVGALSNAA MVDIRAGAQN GYISNIGTYV ANANYIPNQM FCLLLEAPRG FNYLPNPDVQ IGYLKALVEE VAHSITGLQ RGLEVEFVSV PVSGSGEIQE EVSDVKRPRS NPTFGIYEKE GRSVSYFLEQ WITYLLMDPD AKYPMLSSIV S TGGPTDLL ...String:
MRPDNTLLGE QGVGALSNAA MVDIRAGAQN GYISNIGTYV ANANYIPNQM FCLLLEAPRG FNYLPNPDVQ IGYLKALVEE VAHSITGLQ RGLEVEFVSV PVSGSGEIQE EVSDVKRPRS NPTFGIYEKE GRSVSYFLEQ WITYLLMDPD AKYPMLSSIV S TGGPTDLL ADYRSATMLF VEPDRQHKKV VNAWLCTNMM PHGTGDWTSR RNKNDAPNVV ELSIQFTALT QTNYGVRAFA QR LLDKMNL LKVNPDFRPA YLSDIDPAVA AQKVGYTFDF DNNDTFEVPN TYQNVTPRV

UniProtKB: Uncharacterized protein

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Macromolecule #2: Tail sheath protein gp29 gp29PR domain-containing protein

MacromoleculeName: Tail sheath protein gp29 gp29PR domain-containing protein
type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Goslarvirus
Molecular weightTheoretical: 76.777891 KDa
SequenceString: MRLKNSTPRA IFTGFKEGLT TDPVTAPEIT PIHLPYVFIQ GGRGKEDTLL LTGDALSTVY GEDMLNYRSK YASPATLIAR QAASTGSAI FTKRLVAPDA TAARIRIGVE VVADKLVVYQ RNADGSFVKD TLGNKIPDGD KTVDGLKMRW VVNHDKDAET P NAFGKDEV ...String:
MRLKNSTPRA IFTGFKEGLT TDPVTAPEIT PIHLPYVFIQ GGRGKEDTLL LTGDALSTVY GEDMLNYRSK YASPATLIAR QAASTGSAI FTKRLVAPDA TAARIRIGVE VVADKLVVYQ RNADGSFVKD TLGNKIPDGD KTVDGLKMRW VVNHDKDAET P NAFGKDEV VLGQLTATGG AQSNYYPILD GLVSWRGAEG DNIGIRLEAP TALSSNPTRT DIIERIGAFL YRIQFVERKS SR TAPTVIR TISGMEQETF ALQEGVIQPD DETDLSFGKV VVDAYEYNET GNTPIFAPMD QMHLYQKNID DVVKMLYESE RKV NNDLLT DVEFVEGQMN IFTGVDYNGI PYQTIEVLDA DQGGAIFDGT STFYAIDGSD GDVNWDTFDA LAKQQFESFG NMGE DLEDM AFYPFSIVYD IGYKVDTKKA MANVLSLRPD VMIIASTHTW KGVELNVDEE SSMGAMLRNY YRLHPESVLY NTPAC RAAV FMQYGESIEA PSLGKVPLTL EVANKLATYM GASDGVIRGT RIDNNPGNVV TTMRKINKTY RNATVRDRDW ENGLNY VQT YNRNSNFFPA WGTIYNNDRS VWRSLINVII GCDLTRVCYR LWAETTGTTG MDETLFLKLM GERFLEYTTG RYNDHVT VK GRFYLDKEDG YNGFSYHGDI SLIGDPGMTV GTFTITGLRR NTEAAEAA

UniProtKB: Tail sheath protein gp29 gp29PR domain-containing protein

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Macromolecule #3: Tail Adaptor Protein gp50 NTD

MacromoleculeName: Tail Adaptor Protein gp50 NTD / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Goslarvirus
Molecular weightTheoretical: 49.920422 KDa
SequenceString: MALAKKPAKP NINPQQPAKA RDDVYRSAVV DEQYNSQIGL VNYASGYSVE CDYYEQILGR DDEQSSFQAN RDIAEQQYRR IRHYPIKLM SSLTPSYDNG TARFQSEGTC ILPAVSVPLL GNMLLISGLD HRIAVYTITR ITPKNISLAP AYECDISFQR I LDEKQLIE ...String:
MALAKKPAKP NINPQQPAKA RDDVYRSAVV DEQYNSQIGL VNYASGYSVE CDYYEQILGR DDEQSSFQAN RDIAEQQYRR IRHYPIKLM SSLTPSYDNG TARFQSEGTC ILPAVSVPLL GNMLLISGLD HRIAVYTITR ITPKNISLAP AYECDISFQR I LDEKQLIE LEAKIVEDTL YDQDQLKHGK HPVMATEKYN RKYDYLRRAL GLCHAYYHEF YSRELNTLLV PGQDAATYDP WC VNAFTKL WSSLEGKPSW NFGRLNVDDN QEANAISVFE WVMDGRREDE ARLTVKFTLL STKRFYQYPA AGGIRFSGAR FCM WPATTS LGEDVGKELA VPENTPPTNP LVTNLTSMEY DGVPLFPSVA DDPYYVFTEA FYRNQAPMTI IESMVRDMVD EKAI SHPAL KQMVDAIPLL SPLDRYYFTP FVIMLLRAGA IEP

UniProtKB: Virion structural protein

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Macromolecule #4: Tail Terminator protein gp51

MacromoleculeName: Tail Terminator protein gp51 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Goslarvirus
Molecular weightTheoretical: 60.014035 KDa
SequenceString: MPKLDRKIND VYATVTRPVA IAVLRQISEL CGWDYNRENI LFRGEALHAK MSAANEQPPQ VVDQDVKGPA DGFIQIELNI VPEEQASMY DTVYKRNSLP VFEDRNLGVS LVPVYSTMVA TMQITKRFED QVTAERWWYD MKRLLTSQHV AAFLSAEYSY A IPTQFLML ...String:
MPKLDRKIND VYATVTRPVA IAVLRQISEL CGWDYNRENI LFRGEALHAK MSAANEQPPQ VVDQDVKGPA DGFIQIELNI VPEEQASMY DTVYKRNSLP VFEDRNLGVS LVPVYSTMVA TMQITKRFED QVTAERWWYD MKRLLTSQHV AAFLSAEYSY A IPTQFLML LKVIHTHREA VAGYGDKFSE YLRDGFDNRA TTMTTVAGDG KSVKLVIREM QEEISGQFDF TDAPKPEKNE NS SSYNATF SYRYTYDTPT HIVARYPVQV HQQILPEEYL YKPSLSVRDK SRARRGGMVV QYQDILRDSF LPEQGASIQA YRY PDLDEW LPPSSVETEL PSSTLFTALL AIDPEQPNLL VTLDSLAEEG EFHPDLVRWF REYPEWSKCH YQSPFFLEVW KGKY RYARD AWTLTDVGDL EAKDPLDLRS DYHVRLAILL DLSQLQVETL EALCCYPNLV DELFAVLGLD KKYWPKKNSR GCYTL IGLI EAIKRIPTTA SWFKHRSLFV PRILVGTIVG VKRYGAR

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Macromolecule #5: Whiskers Protein gp126

MacromoleculeName: Whiskers Protein gp126 / type: protein_or_peptide / ID: 5 / Number of copies: 10 / Enantiomer: LEVO
Source (natural)Organism: Goslarvirus
Molecular weightTheoretical: 37.008445 KDa
SequenceString: MASVGPIDKT RDAVKALLER IYFELANAEH PSVSLFDDKL TAINNVHPML YQDLLTSWKP MGVFANAFSD TVVANGFEKP KLPFVYEPS VDPRRERLGV LTHALIDSIG YYLSSEWTAK YTELLDEYEL WRNQILTDAE KKAYAVALAT KTRGDVGRLI H DFYAVLKP ...String:
MASVGPIDKT RDAVKALLER IYFELANAEH PSVSLFDDKL TAINNVHPML YQDLLTSWKP MGVFANAFSD TVVANGFEKP KLPFVYEPS VDPRRERLGV LTHALIDSIG YYLSSEWTAK YTELLDEYEL WRNQILTDAE KKAYAVALAT KTRGDVGRLI H DFYAVLKP TYYETKRQLT WEEAQALMQN AETADDVAGI IHDYCMSRIL LDKGNQKYVR EVPLPNNALG IANEIFEFLN EW NPPLLAI TYICDDILTE DLFLPLLGEA SLRRYSQTFK NSATMASTVP VSLPRRRKML TVSAGKINLL VLYYAVRNGL PTL IFRKK

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statehelical array

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Sample preparation

BufferpH: 7.5
GridModel: Quantifoil / Support film - Material: CARBON / Support film - topology: LACEY / Support film - Film thickness: 2
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK II

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOCONTINUUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionApplied symmetry - Point group: C6 (6 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 4.16 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.7.1) / Number images used: 32008
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelChain - Source name: AlphaFold / Chain - Initial model type: in silico model
Output model

PDB-11lc:
Bacteriophage Goslar Collar

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