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- EMDB-75189: HSV-2 gHecto/gL/CHL37-Fab complex -

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Basic information

Entry
Database: EMDB / ID: EMD-75189
TitleHSV-2 gHecto/gL/CHL37-Fab complex
Map dataFull cryoEM generated 3D density map used for gHgL-CHL37 model reconstruction.
Sample
  • Complex: HSV-2 gHecto/gL/CHL37-Fab antibody complex
    • Protein or peptide: Envelope glycoprotein H
    • Protein or peptide: Envelope glycoprotein L
    • Protein or peptide: CHL37-Fab Heavy Chain
    • Protein or peptide: CHL37-Fab Light Chain
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
  • Ligand: water
KeywordsViral Glycoprotein / Fusion Regulator / Antibody Complex / VIRAL PROTEIN-IMMUNE SYSTEM complex
Function / homology
Function and homology information


host cell endosome membrane / host cell Golgi apparatus / fusion of virus membrane with host plasma membrane / viral envelope / symbiont entry into host cell / host cell plasma membrane / virion membrane
Similarity search - Function
Herpesvirus glycoprotein L, C-terminal / dsDNA virus glycoprotein L C terminal / Herpesvirus glycoprotein L, N-terminal / Herpesvirus glycoprotein L, N-terminal domain superfamily / Herpesvirus glycoprotein L / Alphaherpesvirus glycoprotein L (gL) domain profile. / Envelope glycoprotein L / Herpesvirus glycoprotein H main domain / Herpesvirus glycoprotein H / Herpesvirus glycoprotein H, C-terminal ...Herpesvirus glycoprotein L, C-terminal / dsDNA virus glycoprotein L C terminal / Herpesvirus glycoprotein L, N-terminal / Herpesvirus glycoprotein L, N-terminal domain superfamily / Herpesvirus glycoprotein L / Alphaherpesvirus glycoprotein L (gL) domain profile. / Envelope glycoprotein L / Herpesvirus glycoprotein H main domain / Herpesvirus glycoprotein H / Herpesvirus glycoprotein H, C-terminal / Herpesvirus glycoprotein H, C-terminal domain superfamily / Herpesvirus glycoprotein H C-terminal domain
Similarity search - Domain/homology
Envelope glycoprotein L / Envelope glycoprotein H
Similarity search - Component
Biological speciesHuman herpesvirus 2 strain HG52 / Mus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.89 Å
AuthorsRussell HC / Gonzalez-Del Pino GL / Walsh RM / Heldwein EE
Funding support United States, 3 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01 AI164698 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)K12 GM133314 United States
Howard Hughes Medical Institute (HHMI)55108533 United States
CitationJournal: To Be Published
Title: HSV-2 gHecto/gL/CHL37-Fab complex
Authors: Russell HC / Gonzalez-Del Pino GL / Walsh RM / Heldwein EE
History
DepositionJan 21, 2026-
Header (metadata) releaseSep 30, 2026-
Map releaseSep 30, 2026-
UpdateSep 30, 2026-
Current statusSep 30, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75189.png

Downloads & links

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Map

FileDownload / File: emd_75189.map.gz / Format: CCP4 / Size: 371.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationFull cryoEM generated 3D density map used for gHgL-CHL37 model reconstruction.
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.9 Å/pix.
x 460 pix.
= 414. Å		0.9 Å/pix.
x 460 pix.
= 414. Å		0.9 Å/pix.
x 460 pix.
= 414. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.9 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.04
Minimum - Maximum-0.2468159 - 0.36684364
Average (Standard dev.)0.000025287154 (±0.0050484813)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions460460460
Spacing460460460
CellA=B=C: 414.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Half Map 1 of the the cryoEM generated...

Fileemd_75189_half_map_1.map
AnnotationHalf Map 1 of the the cryoEM generated 3D density map used to reconstruct the gHgL-CHL37 Fab Complex.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half Map 2 of the the cryoEM generated...

Fileemd_75189_half_map_2.map
AnnotationHalf Map 2 of the the cryoEM generated 3D density map used to reconstruct the gHgL-CHL37 Fab Complex.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : HSV-2 gHecto/gL/CHL37-Fab antibody complex

EntireName: HSV-2 gHecto/gL/CHL37-Fab antibody complex
Components
  • Complex: HSV-2 gHecto/gL/CHL37-Fab antibody complex
    • Protein or peptide: Envelope glycoprotein H
    • Protein or peptide: Envelope glycoprotein L
    • Protein or peptide: CHL37-Fab Heavy Chain
    • Protein or peptide: CHL37-Fab Light Chain
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
  • Ligand: water

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Supramolecule #1: HSV-2 gHecto/gL/CHL37-Fab antibody complex

SupramoleculeName: HSV-2 gHecto/gL/CHL37-Fab antibody complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4
Source (natural)Organism: Human herpesvirus 2 strain HG52

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Macromolecule #1: Envelope glycoprotein H

MacromoleculeName: Envelope glycoprotein H / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human herpesvirus 2 strain HG52
Molecular weightTheoretical: 86.887961 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MGPGLWVVMG VLVGVAGGHD TYWTEQIDPW FLHGLGLART YWRDTNTGRL WLPNTPDASD PQRGRLAPPG ELNLTTASVP MLRWYAERF CFVLVTTAEF PRDPGQLLYI PKTYLLGRPR NASLPELPEA GPTSRPPAEV TQLKGLSHNP GASALLRSRA W VTFAAAPD ...String:
MGPGLWVVMG VLVGVAGGHD TYWTEQIDPW FLHGLGLART YWRDTNTGRL WLPNTPDASD PQRGRLAPPG ELNLTTASVP MLRWYAERF CFVLVTTAEF PRDPGQLLYI PKTYLLGRPR NASLPELPEA GPTSRPPAEV TQLKGLSHNP GASALLRSRA W VTFAAAPD REGLTFPRGD DGATERHPDG RRNAPPPGPP AGTPRHPTTN LSIAHLHNAS VTWLAARGLL RTPGRYVYLS PS ASTWPVG VWTTGGLAFG CDAALVRARY GKGFMGLVIS MRDSPPAEII VVPADKTLAR VGNPTDENAP AVLPGPPAGP RYR VFVLGA PTPADNGSAL DALRRVAGYP EESTNYAQYM SRAYAEFLGE DPGSGTDARP SLFWRLAGLL ASSGFAFVNA AHAH DAIRL SDLLGFLAHS RVLAGLAARG AAGCAADSVF LNVSVLDPAA RLRLEARLGH LVAAILEREQ SLVAHALGYQ LAFVL DSPA AYGAVAPSAA RLIDALYAEF LGGRALTAPM VRRALFYATA VLRAPFLAGA PSAEQRERAR RGLLITTALC TSDVAA ATH ADLRAALART DHQKNLFWLP DHFSPCAASL RFDLAEGGFI LDALAMATRS DIPADVMAQQ TRGVASVLTR WAHYNAL IR AFVPEATHQC SGPSHNAEPR ILVPITHNAS YVVTHTPLPR GIGYKLTGVD VRRPLFITYL TATCEGHARE IEPKRLVR T ENRRDLGLVG AVFLRYTPAG EVMSVLLVDT DATQQQLAQG PVAGTPNVFS SDVPSVALLL FPNGTVIHLL AFDTLPIAT IAPHHHHHH

UniProtKB: Envelope glycoprotein H

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Macromolecule #2: Envelope glycoprotein L

MacromoleculeName: Envelope glycoprotein L / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human herpesvirus 2 strain HG52
Molecular weightTheoretical: 25.229676 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MGFVCLFGLV VMGAWGAWGG SQATEYVLRS VIAKEVGDIL RVPCMRTPAD DVSWRYEAPS VIDYARIDGI FLRYHCPGLD TFLWDRHAQ RAYLVNPFLF AAGFLEDLSH SVFPADTQET TTRRALYKEI RDALGSRKQA VSHAPVRAGC VNFDYSRTRR C VGRRDLRP ...String:
MGFVCLFGLV VMGAWGAWGG SQATEYVLRS VIAKEVGDIL RVPCMRTPAD DVSWRYEAPS VIDYARIDGI FLRYHCPGLD TFLWDRHAQ RAYLVNPFLF AAGFLEDLSH SVFPADTQET TTRRALYKEI RDALGSRKQA VSHAPVRAGC VNFDYSRTRR C VGRRDLRP ANTTSTWEPP VSSDDEASSQ SKPLATQPPV LALSNAPPRR VSPTRGRRRH TRLRRN

UniProtKB: Envelope glycoprotein L

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Macromolecule #3: CHL37-Fab Heavy Chain

MacromoleculeName: CHL37-Fab Heavy Chain / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 14.905138 KDa
Recombinant expressionOrganism: Mus musculus (house mouse)
SequenceString:
MAVLALLLCL VAFPSCTLSQ VQLKESGPGL VAPSQSLSIT CTVSGFSLAR YDINWIRQPP GKGLEWLGVI WTGGATNYNS AFMSRLSIS KDNSKSQVFL KMNSLLTDDT AIYYCVRGLL LDWYFDVWGA GTTVTVSS

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Macromolecule #4: CHL37-Fab Light Chain

MacromoleculeName: CHL37-Fab Light Chain / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 13.41806 KDa
Recombinant expressionOrganism: Mus musculus (house mouse)
SequenceString:
MAWISLILSL LALSSGAISQ AVVTQESALT TSPGETVTLT CRSSTGAVTT SNYANWVQEK PDHLFTGLIG GTNNRAPGVP ARFSGSLIG DKAALTITGA QTEDEAIYFC GLWYSNHWVF GGGTKLTVL

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Macromolecule #6: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 6 / Number of copies: 3 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

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Macromolecule #7: water

MacromoleculeName: water / type: ligand / ID: 7 / Number of copies: 5 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS GLACIOS
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Average electron dose: 49.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: 4D-STEM / Nominal defocus max: 2.2 µm / Nominal defocus min: 0.8 µm

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

10ie

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.89 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 80128
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE

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