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Basic information

Entry
Database: EMDB / ID: EMD-75133
TitleFcgRIIa in complex with IV.3 Fab
Map dataThe map of FcgRIIa-IV.3 Fab complex
Sample
  • Complex: fcgRIIa-IV.3 Fab complex
    • Complex: fcgRIIa
      • Protein or peptide: Low affinity immunoglobulin gamma Fc region receptor II-a
    • Complex: IV.3 Fab
      • Protein or peptide: IV.3 Fab heavy chain
      • Protein or peptide: IV.3 Fab light chain
Keywordsplatelet / FcgRIIa / mAb IV.3 / IMMUNE SYSTEM
Biological speciesHomo sapiens (human) / Mus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsColler BS / Wang JL
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)5R01HL019278-48 United States
CitationJournal: Biorxiv / Year: 2026
Title: Mechanistic Basis for the Selective Recognition of the Fc gamma Receptor IIa by Monoclonal Antibody IV.3.
Authors: Wang J / Novack S / Li J / Niejadlik EG / Bournazos S / Coller BS / Filizola M
History
DepositionJan 16, 2026-
Header (metadata) releaseJul 1, 2026-
Map releaseJul 1, 2026-
UpdateJul 1, 2026-
Current statusJul 1, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_75133.png

Downloads & links

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Map

FileReleased
AnnotationThe map of FcgRIIa-IV.3 Fab complex
Voxel sizeX=Y=Z: 0.86 Å
Density
Contour LevelBy AUTHOR: 0.6
Minimum - Maximum-6.2420034 - 8.183313
Average (Standard dev.)0.00083191274 (±0.109397516)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions280280280
Spacing280280280
CellA=B=C: 240.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : fcgRIIa-IV.3 Fab complex

EntireName: fcgRIIa-IV.3 Fab complex
Components
  • Complex: fcgRIIa-IV.3 Fab complex
    • Complex: fcgRIIa
      • Protein or peptide: Low affinity immunoglobulin gamma Fc region receptor II-a
    • Complex: IV.3 Fab
      • Protein or peptide: IV.3 Fab heavy chain
      • Protein or peptide: IV.3 Fab light chain

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Supramolecule #1: fcgRIIa-IV.3 Fab complex

SupramoleculeName: fcgRIIa-IV.3 Fab complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Molecular weightTheoretical: 750 KDa

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Supramolecule #2: fcgRIIa

SupramoleculeName: fcgRIIa / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #3: IV.3 Fab

SupramoleculeName: IV.3 Fab / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2-#3
Source (natural)Organism: Mus musculus (house mouse)

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Macromolecule #1: Low affinity immunoglobulin gamma Fc region receptor II-a

MacromoleculeName: Low affinity immunoglobulin gamma Fc region receptor II-a
type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.528963 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
QAAAPPKAVL KLEPPWINVL QEDSVTLTCQ GARSPESDSI QWFHNGNLIP THTQPSYRFK ANNNDSGEYT CQTGQTSLSD PVHLTVLSE WLVLQTPHLE FQEGETIMLR CHSWKDKPLV KVTFFQNGKS QKFSHLDPTF SIPQANHSHS GDYHCTGNIG Y TLFSSKPV TITVQVPSMG SSSPMGI

UniProtKB: Low affinity immunoglobulin gamma Fc region receptor II-a

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Macromolecule #2: IV.3 Fab heavy chain

MacromoleculeName: IV.3 Fab heavy chain / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 25.493609 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MGWSCIILFL VATATGAHSQ IQLVQSGPEL KKPGETVKIS CKASGYTFTN YGMNWVKQAP GKGLKWMGWL NTYTGESIYP DDFKGRFAF SSETSASTAY LQINNLKNED MATYFCARGD YGYDDPLDYW GQGTSVTVSS ASTKGPSVFP LAPSSKSTSG G TAALGCLV ...String:
MGWSCIILFL VATATGAHSQ IQLVQSGPEL KKPGETVKIS CKASGYTFTN YGMNWVKQAP GKGLKWMGWL NTYTGESIYP DDFKGRFAF SSETSASTAY LQINNLKNED MATYFCARGD YGYDDPLDYW GQGTSVTVSS ASTKGPSVFP LAPSSKSTSG G TAALGCLV KDYFPEPVTV SWNSGALTSG VHTFPAVLQS SGLYSLSSVV TVPSSSLGTQ TYICNVNHKP SNTKVDKRVV

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Macromolecule #3: IV.3 Fab light chain

MacromoleculeName: IV.3 Fab light chain / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 25.928209 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MGWSCIILFL VATATGVHSD IVMTQAAPSV PVTPGESVSI SCRSSKSLLH TNGNTYLHWF LQRPGQSPQL LIYRMSVLAS GVPDRFSGS GSGTAFTLSI SRVEAEDVGV FYCMQHLEYP LTFGAGTKLE LKRTVDAPSV FIFPPSDEQL KSGTASVVCL L NNFYPREA ...String:
MGWSCIILFL VATATGVHSD IVMTQAAPSV PVTPGESVSI SCRSSKSLLH TNGNTYLHWF LQRPGQSPQL LIYRMSVLAS GVPDRFSGS GSGTAFTLSI SRVEAEDVGV FYCMQHLEYP LTFGAGTKLE LKRTVDAPSV FIFPPSDEQL KSGTASVVCL L NNFYPREA KVQWKVDNAL QSGNSQESVT EQDSKDSTYS LSSTLTLSKA DYEKHKVYAC EVTHQGLSSP VTKSFNRGEC

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 54.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.2 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. V4.4.0) / Number images used: 392060
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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