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- EMDB-74472: Raw consensus map of RyR1 in presence of rosuvastatin (including ... -

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Basic information

Entry
Database: EMDB / ID: EMD-74472
TitleRaw consensus map of RyR1 in presence of rosuvastatin (including auxiliary transmembrane helices TMx1 and TMx2; Ca2+/CFF/ATP dataset; closed pore)
Map dataRaw consensus in presence of rosuvastatin (including auxiliary transmembrane helices TMx1 and TMx2; Ca/CFF/ATP dataset; closed pore)
Sample
  • Complex: Complex of RyR1 with Calstabin-1 in presence of rosuvastatin (Ca2+/CFF/ATP condition)
KeywordsCalcium / Ion Channel / Membrane Protein / TRANSPORT PROTEIN
Biological speciesOryctolagus cuniculus (rabbit)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.7 Å
AuthorsWeninger G / Marks AR
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)R01 HL178501 United States
CitationJournal: Nat Commun / Year: 2026
Title: Structural basis for rosuvastatin-induced activation of skeletal muscle ryanodine receptor 1
Authors: Weninger G / Reiken S / Yuan Q / Miotto MC / Chang A / Liu Y / Wronska A / Dridi H / Marks AR
History
DepositionDec 15, 2025-
Header (metadata) releaseSep 30, 2026-
Map releaseSep 30, 2026-
UpdateSep 30, 2026-
Current statusSep 30, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_74472.png

Downloads & links

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Map

FileDownload / File: emd_74472.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationRaw consensus in presence of rosuvastatin (including auxiliary transmembrane helices TMx1 and TMx2; Ca/CFF/ATP dataset; closed pore)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.83 Å/pix.
x 512 pix.
= 422.4 Å		0.83 Å/pix.
x 512 pix.
= 422.4 Å		0.83 Å/pix.
x 512 pix.
= 422.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.825 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.1
Minimum - Maximum-0.21300349 - 0.52223605
Average (Standard dev.)0.0010421559 (±0.019459987)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 422.4 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: map sharp

Fileemd_74472_additional_1.map
Annotationmap_sharp
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_74472_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_74472_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Complex of RyR1 with Calstabin-1 in presence of rosuvastatin (Ca2...

EntireName: Complex of RyR1 with Calstabin-1 in presence of rosuvastatin (Ca2+/CFF/ATP condition)
Components
  • Complex: Complex of RyR1 with Calstabin-1 in presence of rosuvastatin (Ca2+/CFF/ATP condition)

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Supramolecule #1: Complex of RyR1 with Calstabin-1 in presence of rosuvastatin (Ca2...

SupramoleculeName: Complex of RyR1 with Calstabin-1 in presence of rosuvastatin (Ca2+/CFF/ATP condition)
type: complex / ID: 1 / Parent: 0
Details: 2 mM rosuvastatin lactone; 0.03 mM free Ca2+; 5 mM Caffeine; 10 mM ATP
Source (natural)Organism: Oryctolagus cuniculus (rabbit)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration8.5 mg/mL
BufferpH: 7.4
Component:
ConcentrationNameFormula
10.0 mmol/LHEPES
400.0 mmol/Lsodium chlorideNaCl
1.0 mmol/LEDTA
0.25 %CHAPS
0.01 %DOPC
0.5 mmol/LTCEP
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 7796 / Average electron dose: 52.95 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.2 µm / Nominal defocus min: 0.5 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL / In silico model: cryoSPARC ab initio
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 142752
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC / Details: cryoSPARC branch-and-bound
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC / Details: cryoSPARC branch-and-bound
FSC plot (resolution estimation)

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Atomic model buiding 1

SoftwareName: Coot

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