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- EMDB-74336: Rabbit muscle aldolase cryo-EM reconstruction obtained from Krios... -

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Basic information

Entry
Database: EMDB / ID: EMD-74336
TitleRabbit muscle aldolase cryo-EM reconstruction obtained from Krios 5 with Selectris-X Falcon 4i detector
Map dataRabbit muscle aldolase cryo-EM reconstruction obtained from Krios 5 with Selectris-X Falcon 4i detector
Sample
  • Complex: Fructose-bisphosphate aldolase A
KeywordsAldolase reductase / protein / Oxidoreductase / Fructose-bisphosphate aldolase A
Biological speciesOryctolagus cuniculus (rabbit)
Methodsingle particle reconstruction / cryo EM / Resolution: 1.5 Å
AuthorsKoh A / Rai J / Karia D / Yu L / Raschdorf O
Funding supportNauru, 1 items
OrganizationGrant numberCountry
Other privateNauru
CitationJournal: To Be Published
Title: Rabbit muscle aldolase cryo-EM reconstruction obtained from Krios 5 with Selectris-X Falcon 4i detector
Authors: Adrian K / Rai J / Karia D / Yu L / Raschdorf O
History
DepositionDec 5, 2025-
Header (metadata) releaseDec 17, 2025-
Map releaseDec 17, 2025-
UpdateDec 17, 2025-
Current statusDec 17, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_74336.png

Downloads & links

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Map

FileDownload / File: emd_74336.map.gz / Format: CCP4 / Size: 343 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationRabbit muscle aldolase cryo-EM reconstruction obtained from Krios 5 with Selectris-X Falcon 4i detector
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.45 Å/pix.
x 448 pix.
= 203.392 Å		0.45 Å/pix.
x 448 pix.
= 203.392 Å		0.45 Å/pix.
x 448 pix.
= 203.392 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.454 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0496
Minimum - Maximum-0.3150929 - 0.62310714
Average (Standard dev.)-0.000029301409 (±0.011781137)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions448448448
Spacing448448448
CellA=B=C: 203.392 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Additional Map

Fileemd_74336_additional_1.map
AnnotationAdditional Map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half Map B

Fileemd_74336_half_map_1.map
AnnotationHalf Map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half Map A

Fileemd_74336_half_map_2.map
AnnotationHalf Map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Fructose-bisphosphate aldolase A

EntireName: Fructose-bisphosphate aldolase A
Components
  • Complex: Fructose-bisphosphate aldolase A

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Supramolecule #1: Fructose-bisphosphate aldolase A

SupramoleculeName: Fructose-bisphosphate aldolase A / type: complex / ID: 1 / Parent: 0 / Details: Purchased as a lyophilized powder (Sigma-Aldrich)
Source (natural)Organism: Oryctolagus cuniculus (rabbit) / Tissue: Muscle

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
GridModel: UltrAuFoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Support film - Material: GOLD / Support film - topology: HOLEY / Support film - Film thickness: 5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K

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Electron microscopy

MicroscopeTFS KRIOS
Specialist opticsEnergy filter - Name: TFS Selectris X / Energy filter - Slit width: 10 eV
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Number real images: 11501 / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.0 µm / Nominal defocus min: 0.5 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 1.5 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. v4.7.1) / Number images used: 394615
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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