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- EMDB-74335: Rabbit muscle aldolase cryo-EM reconstruction obtained from Glaci... -

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Basic information

Entry
Database: EMDB / ID: EMD-74335
TitleRabbit muscle aldolase cryo-EM reconstruction obtained from Glacios 3 with Selectris-X Falcon 4i detector
Map dataRabbit muscle aldolase cryo-EM reconstruction obtained from Glacios 3 with Selectris-X Falcon 4i detector
Sample
  • Complex: Fructose-bisphosphate aldolase A
KeywordsAldolase reductase / protein / Oxidoreductase / Fructose-bisphosphate aldolase A
Biological speciesOryctolagus cuniculus (rabbit)
Methodsingle particle reconstruction / cryo EM / Resolution: 1.94 Å
AuthorsKoh A / Rai J / Karia D / Yu L / Raschdorf O
Funding support Netherlands, 1 items
OrganizationGrant numberCountry
Other private Netherlands
CitationJournal: To Be Published
Title: Rabbit muscle aldolase cryo-EM reconstruction obtained from Glacios 3 with Selectris-X Falcon 4i detector
Authors: Adrian K / Rai J / Karia D / Yu L / Raschdorf O
History
DepositionDec 5, 2025-
Header (metadata) releaseApr 8, 2026-
Map releaseApr 8, 2026-
UpdateApr 8, 2026-
Current statusApr 8, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_74335.png

Downloads & links

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Map

FileDownload / File: emd_74335.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationRabbit muscle aldolase cryo-EM reconstruction obtained from Glacios 3 with Selectris-X Falcon 4i detector
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.64 Å/pix.
x 300 pix.
= 190.959 Å		0.64 Å/pix.
x 300 pix.
= 190.959 Å		0.64 Å/pix.
x 300 pix.
= 190.959 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.63653 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.12
Minimum - Maximum-0.5087557 - 0.79626834
Average (Standard dev.)-0.000084379324 (±0.022205899)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 190.959 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Additional Map

Fileemd_74335_additional_1.map
AnnotationAdditional Map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half Map A

Fileemd_74335_half_map_1.map
AnnotationHalf Map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half Map B

Fileemd_74335_half_map_2.map
AnnotationHalf Map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Fructose-bisphosphate aldolase A

EntireName: Fructose-bisphosphate aldolase A
Components
  • Complex: Fructose-bisphosphate aldolase A

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Supramolecule #1: Fructose-bisphosphate aldolase A

SupramoleculeName: Fructose-bisphosphate aldolase A / type: complex / ID: 1 / Parent: 0 / Details: Purchased as a lyophilized powder (Sigma-Aldrich)
Source (natural)Organism: Oryctolagus cuniculus (rabbit) / Tissue: Muscle
Molecular weightTheoretical: 156 KDa

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
GridModel: UltrAuFoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Support film - Material: GOLD / Support film - topology: HOLEY / Support film - Film thickness: 5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS GLACIOS
Specialist opticsEnergy filter - Name: TFS Selectris X / Energy filter - Slit width: 10 eV
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Number real images: 8625 / Average electron dose: 60.0 e/Å2
Details: Original Pixel size is 0.434 A/pix. During image processing, the pixel was Fourier cropped to 0.63 A/pix
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.0 µm / Nominal defocus min: 0.6 µm

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionApplied symmetry - Point group: D2 (2x2 fold dihedral) / Resolution.type: BY AUTHOR / Resolution: 1.94 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC
Details: Original Pixel size is 0.434 A/pix. During image processing, the pixel was Fourier cropped to 0.63 A/pix
Number images used: 386680
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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