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- EMDB-74262: Cryo-electron tomography and subtomogram averaging of Leishmania ... -

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Basic information

Entry
Database: EMDB / ID: EMD-74262
TitleCryo-electron tomography and subtomogram averaging of Leishmania tarentolae Central Pair Complex
Map dataPrimary map of Leishmania tarentolae central pair complex
Sample
  • Organelle or cellular component: Leishmania tarentolae flagella central pair complex
Keywordsflagella / motility / microtubules / mechanical signaling / STRUCTURAL PROTEIN
Biological speciesLeishmania tarentolae (eukaryote)
Methodsubtomogram averaging / cryo EM / Resolution: 35.0 Å
AuthorsWilburn D / Nicastro D
Funding support United States, 3 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01 GM083122 United States
Cancer Prevention and Research Institute of Texas (CPRIT)RR140082 United States
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)F32 HL178175 United States
CitationJournal: Proceedings of the National Academy of Sciences / Year: 2026
Title: Unveiling the structure of Leishmania flagella through cryoelectron tomography
Authors: Wilburn D / Pinskey J / Zhu H / Nepal B / Phan N / Wetzel DM / Nicastro D
History
DepositionDec 3, 2025-
Header (metadata) releaseOct 28, 2026-
Map releaseOct 28, 2026-
UpdateOct 28, 2026-
Current statusOct 28, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_74262.png

Downloads & links

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Map

FileDownload / File: emd_74262.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationPrimary map of Leishmania tarentolae central pair complex
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
3.15 Å/pix.
x 300 pix.
= 945.3 Å		3.15 Å/pix.
x 300 pix.
= 945.3 Å		3.15 Å/pix.
x 300 pix.
= 945.3 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 3.151 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 133.0
Minimum - Maximum116.182879999999997 - 147.426680000000005
Average (Standard dev.)130.002440000000007 (±2.7919586)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 945.3 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Half map1 of Leishmania tarentolae central pair complex

Fileemd_74262_half_map_1.map
AnnotationHalf map1 of Leishmania tarentolae central pair complex
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map2 of Leishmania tarentolae central pair complex

Fileemd_74262_half_map_2.map
AnnotationHalf map2 of Leishmania tarentolae central pair complex
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Leishmania tarentolae flagella central pair complex

EntireName: Leishmania tarentolae flagella central pair complex
Components
  • Organelle or cellular component: Leishmania tarentolae flagella central pair complex

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Supramolecule #1: Leishmania tarentolae flagella central pair complex

SupramoleculeName: Leishmania tarentolae flagella central pair complex / type: organelle_or_cellular_component / ID: 1 / Parent: 0
Source (natural)Organism: Leishmania tarentolae (eukaryote)

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statecell

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 1.6 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 5.0 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 35.0 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: PEET / Number subtomograms used: 544
ExtractionNumber tomograms: 11 / Number images used: 544 / Software - Name: PEET
CTF correctionType: NONE
Final angle assignmentType: OTHER
Details: Angles (and shifts) for each subvolume are assigned by maximizing normalized cross-correlation between the subvolume and the reference

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