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- EMDB-73781: CryoEM Structure of VEEV VLP treated with 3.0 biFABs/Spike -

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Basic information

Entry
Database: EMDB / ID: EMD-73781
TitleCryoEM Structure of VEEV VLP treated with 3.0 biFABs/Spike
Map data
Sample
  • Complex: CryoEM Structure of VEEV VLP treated with 3.0 biFABs/Spike
Keywordsalpha virus / VEEV / antibody / VIRUS LIKE PARTICLE
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 20.17 Å
AuthorsMorano NC / Pletnev S / Shapiro L / Kwong PD
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) United States
CitationJournal: To Be Published
Title: CRYO-EM STRUCTURE OF HIV-1 BG505DS-SOSIP.664 ENV TRIMER BOUND TO HERH-c.01 FAB
Authors: Morano NC / Hoyt F / Hansen B / Fischer E / Shapiro L
History
DepositionNov 5, 2025-
Header (metadata) releaseJul 22, 2026-
Map releaseJul 22, 2026-
UpdateJul 22, 2026-
Current statusJul 22, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_73781.png

Downloads & links

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Map

FileReleased
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.83 Å/pix.
x 800 pix.
= 660. Å		0.83 Å/pix.
x 800 pix.
= 660. Å		0.83 Å/pix.
x 800 pix.
= 660. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.825 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0267
Minimum - Maximum-0.03833051 - 0.08876069
Average (Standard dev.)0.0024083334 (±0.014517769)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions800800800
Spacing800800800
CellA=B=C: 660.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_73781_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_73781_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : CryoEM Structure of VEEV VLP treated with 3.0 biFABs/Spike

EntireName: CryoEM Structure of VEEV VLP treated with 3.0 biFABs/Spike
Components
  • Complex: CryoEM Structure of VEEV VLP treated with 3.0 biFABs/Spike

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Supramolecule #1: CryoEM Structure of VEEV VLP treated with 3.0 biFABs/Spike

SupramoleculeName: CryoEM Structure of VEEV VLP treated with 3.0 biFABs/Spike
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20 MDa

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration4 mg/mL
BufferpH: 7
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 58.06 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 20.17 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 6539
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE

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