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- EMDB-73133: Benchmarking Talos Arctica with Falcon3 in linear mode (25ms/frame) -

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Basic information

Entry
Database: EMDB / ID: EMD-73133
TitleBenchmarking Talos Arctica with Falcon3 in linear mode (25ms/frame)
Map data
Sample
  • Organelle or cellular component: Apoferritin
    • Protein or peptide: Apoferritin
Keywordsmouse Apoferritin / mFTH1 / Iron binding protein / METAL BINDING PROTEIN
Biological speciesMus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.1 Å
AuthorsMaruthi K / Morais M
Funding support United States, 1 items
OrganizationGrant numberCountry
Other governmentNIH grant, USA United States
CitationJournal: To Be Published
Title: Benchmarking Talos Arctica with Falcon3 in linear mode (25ms/frame)
Authors: Maruthi K / Morais M
History
DepositionOct 10, 2025-
Header (metadata) releaseOct 22, 2025-
Map releaseOct 22, 2025-
UpdateOct 22, 2025-
Current statusOct 22, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_73133.png

Downloads & links

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Map

FileDownload / File: emd_73133.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.97 Å/pix.
x 256 pix.
= 248.064 Å		0.97 Å/pix.
x 256 pix.
= 248.064 Å		0.97 Å/pix.
x 256 pix.
= 248.064 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.969 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.57
Minimum - Maximum-0.40165776 - 1.473183
Average (Standard dev.)-0.0025015697 (±0.10926034)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 248.064 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_73133_msk_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
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Histogram

Histogram (log scale)

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Additional map: #1

Fileemd_73133_additional_1.map
Projections & Slices
AxesZYX

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Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_73133_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_73133_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Apoferritin

EntireName: Apoferritin
Components
  • Organelle or cellular component: Apoferritin
    • Protein or peptide: Apoferritin

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Supramolecule #1: Apoferritin

SupramoleculeName: Apoferritin / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Mus musculus (house mouse)

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Macromolecule #1: Apoferritin

MacromoleculeName: Apoferritin / type: protein_or_peptide / ID: 1 / Details: https://pubmed.ncbi.nlm.nih.gov/31078399/ / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: GSHMASMTGG QQMGRGSMTT ASPSQVRQNY HQDAEAAINR QINLELYASY VYLSMSCYFD RDDVALKNFA KYFLHQSHEE REHAEKLMKL QNQRGGRIFL QDIKKPDRDD WESGLNAMEC ALHLEKSVNQ SLLELHKLAT DKNDPHLCDF IETYYLSEQV KSIKELGDHV ...String:
GSHMASMTGG QQMGRGSMTT ASPSQVRQNY HQDAEAAINR QINLELYASY VYLSMSCYFD RDDVALKNFA KYFLHQSHEE REHAEKLMKL QNQRGGRIFL QDIKKPDRDD WESGLNAMEC ALHLEKSVNQ SLLELHKLAT DKNDPHLCDF IETYYLSEQV KSIKELGDHV TNLRKMGAPE AGMAEYLFDK HTLGHGDESG DIESAQSDEE VE

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
GridMaterial: GOLD
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 298 K / Instrument: FEI VITROBOT MARK III

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average exposure time: 3.0 sec. / Average electron dose: 48.17 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 150000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.1 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 298110
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
FSC plot (resolution estimation)

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