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- EMDB-72954: Trans-1-azomorphine-3 bound mu opioid receptor Gi complex - Focus... -

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Basic information

Entry
Database: EMDB / ID: EMD-72954
TitleTrans-1-azomorphine-3 bound mu opioid receptor Gi complex - Focused receptor map
Map data
Sample
  • Complex: Trans-azomorphine-3 bound Mu opioid receptor in complex with heterotrimeric G protein (Gai/b/g)
KeywordsMu Opioid Receptor / G protein coupled receptor / trans-azomorphine-3 / MEMBRANE PROTEIN
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.13 Å
AuthorsGati C / Khan S / Han GW
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM144965 United States
National Institutes of Health/National Center for Complementary and Integrative Health (NIH/NCCIH)AT012075 United States
CitationJournal: To Be Published
Title: An opioid efficacy switch for reversible optical control of peripheral analgesia
Authors: Posa L / Romano G / Ji X / Khan S / Paz B / Han GW / Nazarova A / Zaidi S / Pleil K / Katritch V / Gati C / Trauner D / Levitz J
History
DepositionSep 30, 2025-
Header (metadata) releaseOct 14, 2026-
Map releaseOct 14, 2026-
UpdateOct 14, 2026-
Current statusOct 14, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_72954.png

Downloads & links

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Map

FileDownload / File: emd_72954.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.86 Å/pix.
x 384 pix.
= 331.008 Å		0.86 Å/pix.
x 384 pix.
= 331.008 Å		0.86 Å/pix.
x 384 pix.
= 331.008 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.862 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.375
Minimum - Maximum-2.9680963 - 3.4717128
Average (Standard dev.)-0.0005545107 (±0.026492244)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 331.008 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_72954_additional_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_72954_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_72954_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Trans-azomorphine-3 bound Mu opioid receptor in complex with hete...

EntireName: Trans-azomorphine-3 bound Mu opioid receptor in complex with heterotrimeric G protein (Gai/b/g)
Components
  • Complex: Trans-azomorphine-3 bound Mu opioid receptor in complex with heterotrimeric G protein (Gai/b/g)

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Supramolecule #1: Trans-azomorphine-3 bound Mu opioid receptor in complex with hete...

SupramoleculeName: Trans-azomorphine-3 bound Mu opioid receptor in complex with heterotrimeric G protein (Gai/b/g)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 150 kDa/nm

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1.1 mg/mL
BufferpH: 7.5
VitrificationCryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOCONTINUUM (6k x 4k) / Number grids imaged: 1 / Average electron dose: 51.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 130000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL / In silico model: AB INITIO
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.13 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. v4.3.1) / Number images used: 284398
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: cryoSPARC (ver. v4.3.1)
Final angle assignmentType: ANGULAR RECONSTITUTION
FSC plot (resolution estimation)

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