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- EMDB-72930: Plasmodium falciparum moving junction staple - Particle subtracte... -

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Basic information

Entry
Database: EMDB / ID: EMD-72930
TitlePlasmodium falciparum moving junction staple - Particle subtracted, local refined C-terminus of PfRON5
Map dataLocal Refined C-terminus of PfRON5
Sample
  • Complex: Plasmodium falciparum moving junction staple with PfAMA1 - PfRON2 - PfRON4 - PfRON5
KeywordsInvasion / Plasmodium / Apicomplexa / CryoEM / Endogenous / MEMBRANE PROTEIN
Biological speciesPlasmodium falciparum NF54 (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsHaile MT / Zhen J / Ho C
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/Office of the DirectorDP5OD029613 United States
CitationJournal: Cell / Year: 2026
Title: Structural basis for host membrane binding and remodeling by invading malaria parasites.
Authors: Meseret T Haile / Daphne A Kaxiras / James Zhen / Carolyn L Lee / Britney Jiang / Jennifer L Small-Saunders / Chi-Min Ho /
Abstract: The Plasmodium moving junction is central to malarial host-cell invasion, and yet its function remains unclear. Here, we determine the endogenous structure of the basic repeating unit of the moving ...The Plasmodium moving junction is central to malarial host-cell invasion, and yet its function remains unclear. Here, we determine the endogenous structure of the basic repeating unit of the moving junction, purified directly from invasion-stalled Plasmodium falciparum parasites, revealing a sailboat-shaped 1:1:1:1 assembly of apical membrane antigen 1 (PfAMA1) and rhoptry neck proteins 2, 4, and 5 (PfRON2, PfRON4, and PfRON5). We observe two PfRON2 transmembrane helices that anchor the complex in the red blood cell (RBC) membrane and display an extracellular handle for PfAMA1 binding. PfAMA1 directly contacts the RBC membrane, strengthening the connection. PfRON2/4/5 form a large, basic platform inside the RBC that electrostatically engages the RBC membrane and wedges seven amphipathic helices deep into the bilayer, suggesting an active role in host-membrane remodeling. We then leverage the native membrane context revealed by our structure, along with recent advances in computational protein design, to enable the rational design of a small protein binder that inhibits invasion.
History
DepositionSep 29, 2025-
Header (metadata) releaseJul 1, 2026-
Map releaseJul 1, 2026-
UpdateJul 15, 2026-
Current statusJul 15, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_72930.png

Downloads & links

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Map

FileDownload / File: emd_72930.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationLocal Refined C-terminus of PfRON5
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.82 Å/pix.
x 256 pix.
= 210.688 Å		0.82 Å/pix.
x 256 pix.
= 210.688 Å		0.82 Å/pix.
x 256 pix.
= 210.688 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.823 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.056
Minimum - Maximum-0.12115061 - 0.2408854
Average (Standard dev.)0.00042505725 (±0.0050508175)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 210.688 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Half map A of Local Refined C-terminus of PfRON5

Fileemd_72930_half_map_1.map
AnnotationHalf map A of Local Refined C-terminus of PfRON5
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map B of Local Refined C-terminus of PfRON5

Fileemd_72930_half_map_2.map
AnnotationHalf map B of Local Refined C-terminus of PfRON5
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Plasmodium falciparum moving junction staple with PfAMA1 - PfRON2...

EntireName: Plasmodium falciparum moving junction staple with PfAMA1 - PfRON2 - PfRON4 - PfRON5
Components
  • Complex: Plasmodium falciparum moving junction staple with PfAMA1 - PfRON2 - PfRON4 - PfRON5

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Supramolecule #1: Plasmodium falciparum moving junction staple with PfAMA1 - PfRON2...

SupramoleculeName: Plasmodium falciparum moving junction staple with PfAMA1 - PfRON2 - PfRON4 - PfRON5
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4
Source (natural)Organism: Plasmodium falciparum NF54 (eukaryote)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7
GridModel: Quantifoil / Material: GOLD / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 0.2 / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOCONTINUUM (6k x 4k) / Average electron dose: 58.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4) / Number images used: 184298
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementProtocol: AB INITIO MODEL

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