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- EMDB-72759: Structure of Measles virus fusion protein in pre-fusion in comple... -

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Basic information

Entry
Database: EMDB / ID: EMD-72759
TitleStructure of Measles virus fusion protein in pre-fusion in complex with neutralizing Fabs MeV-15 and MeV-104
Map data
Sample
  • Complex: Structure of Measles virus fusion protein in pre-fusion in complex with neutralizing Fabs MeV-15 and MeV-104
    • Complex: Measles virus fusion protein
      • Protein or peptide: Fusion glycoprotein F2
      • Protein or peptide: Fusion glycoprotein F1
    • Complex: Fab MeV-15
      • Protein or peptide: MeV-15 Fab Heavy Chain
      • Protein or peptide: MeV-15 Fab Light Chain
    • Complex: Fab MeV-104
      • Protein or peptide: MeV-104 Fab Heavy Chain
      • Protein or peptide: MeV-104 Fab Light Chain
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
KeywordsMeasles / mAb / fusion / VIRAL PROTEIN
Function / homologyPrecursor fusion glycoprotein F0, Paramyxoviridae / Fusion glycoprotein F0 / fusion of virus membrane with host plasma membrane / viral envelope / symbiont entry into host cell / host cell plasma membrane / virion membrane / Fusion glycoprotein F0
Function and homology information
Biological speciesMeasles morbillivirus / Homo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.9 Å
AuthorsBinshtein E / Crowe JE
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: To Be Published
Title: Structure of Measles virus fusion protein in pre-fusion in complex with neutralizing Fabs MeV-15 and MeV-104
Authors: Binshtein E / Crowe JE
History
DepositionSep 17, 2025-
Header (metadata) releaseSep 2, 2026-
Map releaseSep 2, 2026-
UpdateSep 2, 2026-
Current statusSep 2, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_72759.png

Downloads & links

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Map

FileDownload / File: emd_72759.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.65 Å/pix.
x 420 pix.
= 271.74 Å		0.65 Å/pix.
x 420 pix.
= 271.74 Å		0.65 Å/pix.
x 420 pix.
= 271.74 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.647 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.1
Minimum - Maximum-0.0018198611 - 1.73055
Average (Standard dev.)0.0023385137 (±0.033080846)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions420420420
Spacing420420420
CellA=B=C: 271.74002 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_72759_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_72759_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Structure of Measles virus fusion protein in pre-fusion in comple...

EntireName: Structure of Measles virus fusion protein in pre-fusion in complex with neutralizing Fabs MeV-15 and MeV-104
Components
  • Complex: Structure of Measles virus fusion protein in pre-fusion in complex with neutralizing Fabs MeV-15 and MeV-104
    • Complex: Measles virus fusion protein
      • Protein or peptide: Fusion glycoprotein F2
      • Protein or peptide: Fusion glycoprotein F1
    • Complex: Fab MeV-15
      • Protein or peptide: MeV-15 Fab Heavy Chain
      • Protein or peptide: MeV-15 Fab Light Chain
    • Complex: Fab MeV-104
      • Protein or peptide: MeV-104 Fab Heavy Chain
      • Protein or peptide: MeV-104 Fab Light Chain
  • Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose

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Supramolecule #1: Structure of Measles virus fusion protein in pre-fusion in comple...

SupramoleculeName: Structure of Measles virus fusion protein in pre-fusion in complex with neutralizing Fabs MeV-15 and MeV-104
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#6

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Supramolecule #2: Measles virus fusion protein

SupramoleculeName: Measles virus fusion protein / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#2
Source (natural)Organism: Measles morbillivirus

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Supramolecule #3: Fab MeV-15

SupramoleculeName: Fab MeV-15 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #3-#4
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #4: Fab MeV-104

SupramoleculeName: Fab MeV-104 / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #5-#6
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Fusion glycoprotein F2

MacromoleculeName: Fusion glycoprotein F2 / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Measles morbillivirus
Molecular weightTheoretical: 12.498768 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MGLKVNVSAI FMAVLLTLQT PTGQIHWGNL SKIGVVGIGS ASYKVMTRSS HQSLVIKLMP NITLLNNCTR VEIAEYRRLL RTVLEPIRD ALNAMTQNIR PVQSVASSRR HKR

UniProtKB: Fusion glycoprotein F0

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Macromolecule #2: Fusion glycoprotein F1

MacromoleculeName: Fusion glycoprotein F1 / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Measles morbillivirus
Molecular weightTheoretical: 41.310129 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: FAGVVLAGAA LGVATAAQIT AGIALHQSML NSQAIDNLRA SLETTNQAIE AIRQAGQEMI LAVQGVQDYI NNELIPSMNQ LSCDLIGQK LGLKLLRYYT EILSLFGPSL RDPISAEISI QALSYALGGD INKVLEKLGY SGGDLLGILE SRGIKARITH V DTESYFIV ...String:
FAGVVLAGAA LGVATAAQIT AGIALHQSML NSQAIDNLRA SLETTNQAIE AIRQAGQEMI LAVQGVQDYI NNELIPSMNQ LSCDLIGQK LGLKLLRYYT EILSLFGPSL RDPISAEISI QALSYALGGD INKVLEKLGY SGGDLLGILE SRGIKARITH V DTESYFIV LSIAYPTLSE IKGVIVHRLE GVSYNIGSQE WYTTVPKYVA TQGYLISNFD ESSCTFMPEG TVCSQNALYP MS PLLQECL RGSTKSCART LVSGSFGNRF ILSQGNLIAN CASILCKCYT TGTIINQDPD KILTYIAADH CPVVEVNGVT IQV GSRRYP DAVYLHRIDL GPPISLERLD VGTNLGNAIA KLEDAKELLE SSDQILRSMK GLSSTSI

UniProtKB: Fusion glycoprotein F0

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Macromolecule #3: MeV-15 Fab Heavy Chain

MacromoleculeName: MeV-15 Fab Heavy Chain / type: protein_or_peptide / ID: 3 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 13.310938 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
QVQLVQSGAE VKKPGASVRV SCKASAYSFI PYYMHWVRQA PGQGLEWMGR INPSGTDTTY AQKLQGRLIM TTDTSTNTVY MELSSLRSE DTAVYYCARS RYSSGWYVYW GQGTLVTVSS

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Macromolecule #4: MeV-15 Fab Light Chain

MacromoleculeName: MeV-15 Fab Light Chain / type: protein_or_peptide / ID: 4 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 11.848061 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
DIQMTQSPSS LSASVGDRVT ITCQASQDIS NYLNWYQQKP GKAPKLLIYD ASNLETGVPS RFSGSGSGTD FTFTISSLQP EDIATYYCQ QYDHLPFTFG RGTRLEIK

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Macromolecule #5: MeV-104 Fab Heavy Chain

MacromoleculeName: MeV-104 Fab Heavy Chain / type: protein_or_peptide / ID: 5 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 13.605054 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
EVQLVESGGG LVKPGGSLRL SCAASGFTFS DFYMSWVRQA PGKGLEWVSY ISTSSSYRNY ADSVKGRFTI SRDDATNSLY LQMNSLRAE DTAVYYCARG VGSDWYRGVY FDYWGQGILV TVSS

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Macromolecule #6: MeV-104 Fab Light Chain

MacromoleculeName: MeV-104 Fab Light Chain / type: protein_or_peptide / ID: 6 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 11.95425 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
DIQMTQSPST LSASVGDRVT ITCRASQSFG NYLAWYQQKP GKAPKLLIYR ASYLQSGVPS RFSGSGSETE FTLTISTLQP DDFATYYCQ QYNRYSQTFG QGTKVEIK

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Macromolecule #8: 2-acetamido-2-deoxy-beta-D-glucopyranose

MacromoleculeName: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 8 / Number of copies: 3 / Formula: NAG
Molecular weightTheoretical: 221.208 Da
Chemical component information

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
GridModel: Quantifoil
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 2 / Number real images: 10000 / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 130000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 36025
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementProtocol: AB INITIO MODEL
Output model

PDB-9yby:
Structure of Measles virus fusion protein in pre-fusion in complex with neutralizing Fabs MeV-15 and MeV-104

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