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- EMDB-71733: Focused map for TPR on CC1/4 and KLC-CC -

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Open data


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Basic information

Entry
Database: EMDB / ID: EMD-71733
TitleFocused map for TPR on CC1/4 and KLC-CC
Map dataFocused map for TPR on CC1/4 and KLC-CC
Sample
  • Cell: Heterotetramer of KIF5B/KLC1
KeywordsAuto-inhibited Kinesin-1 heterotetramer / MOTOR PROTEIN
Biological speciesInsect cell expression vector pTIE1 (others)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.56 Å
Authorsashaduzzaman M / Al-Bassam J
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: To Be Published
Title: Focused map TPR on CC1/4 and KLC-CC
Authors: ashaduzzaman M / Al-Bassam J
History
DepositionJul 16, 2025-
Header (metadata) releaseJul 22, 2026-
Map releaseJul 22, 2026-
UpdateJul 22, 2026-
Current statusJul 22, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_71733.png

Downloads & links

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Map

FileReleased
AnnotationFocused map for TPR on CC1/4 and KLC-CC
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.85 Å/pix.
x 150 pix.
= 277.8 Å		1.85 Å/pix.
x 150 pix.
= 277.8 Å		1.85 Å/pix.
x 150 pix.
= 277.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.852 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.2
Minimum - Maximum-0.0002794213 - 2.557858
Average (Standard dev.)0.003213912 (±0.042770054)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions150150150
Spacing150150150
CellA=B=C: 277.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Half Map A

Fileemd_71733_half_map_1.map
AnnotationHalf Map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half Map B

Fileemd_71733_half_map_2.map
AnnotationHalf Map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Heterotetramer of KIF5B/KLC1

EntireName: Heterotetramer of KIF5B/KLC1
Components
  • Cell: Heterotetramer of KIF5B/KLC1

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Supramolecule #1: Heterotetramer of KIF5B/KLC1

SupramoleculeName: Heterotetramer of KIF5B/KLC1 / type: cell / ID: 1 / Parent: 0
Source (natural)Organism: Insect cell expression vector pTIE1 (others) / Strain: sf9

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 48.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: RELION / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 5.56 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 595495
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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