|Entry||Database: EMDB / ID: 7118|
|Title||Open state structure of the full-length TRPV2 cation channel with a resolved pore turret domain|
|Map data||WT rat TRPV2 ion channel in an open state and with a resolved pore turret domain.|
|Function / homology||Ankyrin repeat / Transient receptor potential cation channel subfamily V / Ankyrin repeat region circular profile. / Ankyrin repeat profile. / Ankyrin repeats (3 copies) / Ion transport protein / Ankyrin repeat-containing domain superfamily / Transient receptor potential channel, vanilloid 2 / Ion transport domain / Transient receptor potential channel, vanilloid 1-4 ...Ankyrin repeat / Transient receptor potential cation channel subfamily V / Ankyrin repeat region circular profile. / Ankyrin repeat profile. / Ankyrin repeats (3 copies) / Ion transport protein / Ankyrin repeat-containing domain superfamily / Transient receptor potential channel, vanilloid 2 / Ion transport domain / Transient receptor potential channel, vanilloid 1-4 / Ankyrin repeat-containing domain / endomembrane system / calcium channel activity / positive regulation of cold-induced thermogenesis / melanosome / lamellipodium / ion channel activity / response to heat / negative regulation of cell population proliferation / integral component of plasma membrane / identical protein binding / plasma membrane / cytoplasm / Transient receptor potential cation channel subfamily V member 2|
Function and homology information
|Source||Rattus norvegicus (Norway rat)|
|Method||single particle reconstruction / cryo EM / 4 Å resolution|
|Authors||Dosey TL / Wang Z|
|Citation||Journal: Nat. Struct. Mol. Biol. / Year: 2019|
Title: Structures of TRPV2 in distinct conformations provide insight into role of the pore turret.
Authors: Timothy L Dosey / Zhao Wang / Guizhen Fan / Zhixian Zhang / Irina I Serysheva / Wah Chiu / Theodore G Wensel
|Validation Report||PDB-ID: 6bo4|
SummaryFull reportAbout validation report
|Date||Deposition: Nov 18, 2017 / Header (metadata) release: Jan 24, 2018 / Map release: Dec 5, 2018 / Last update: Dec 5, 2018|
|Structure viewer||EM map: |
Downloads & links
|File||emd_7118.map.gz (map file in CCP4 format, 16385 KB)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 1.25 Å|
CCP4 map header:
|Entire||Name: TRPV2 / Number of components: 2|
-Component #1: protein, TRPV2
|Protein||Name: TRPV2 / Recombinant expression: No|
|Mass||Theoretical: 320 kDa|
|Source||Species: Rattus norvegicus (Norway rat)|
|Source (engineered)||Expression System: Saccharomyces cerevisiae (baker's yeast) / Vector: pYEPM / Strain: BJ5457|
-Component #2: protein, Transient receptor potential cation channel subfamily V ...
|Protein||Name: Transient receptor potential cation channel subfamily V member 2|
Number of Copies: 4 / Recombinant expression: No
|Mass||Theoretical: 79.999422 kDa|
|Source||Species: Rattus norvegicus (Norway rat)|
|Source (engineered)||Expression System: Saccharomyces cerevisiae (baker's yeast)|
|Specimen||Specimen state: particle / Method: cryo EM|
|Sample solution||Specimen conc.: .5 mg/ml / pH: 8|
|Vitrification||Cryogen name: ETHANE / Temperature: 298 K / Humidity: 98 %|
-Electron microscopy imaging
Model: Tecnai Polara / Image courtesy: FEI Company
|Imaging||Microscope: FEI POLARA 300|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 63 e/Å2 / Illumination mode: SPOT SCAN|
|Lens||Imaging mode: BRIGHT FIELD|
|Specimen Holder||Model: OTHER|
|Camera||Detector: GATAN K2 SUMMIT (4k x 4k)|
|Processing||Method: single particle reconstruction / Applied symmetry: C4 (4 fold cyclic) / Number of projections: 11789|
|3D reconstruction||Software: RELION / Resolution: 4 Å / Resolution method: FSC 0.143 CUT-OFF|
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