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- EMDB-71069: Flavobacterium johnsoniae ZAM64 mutant 70S initiation complex wit... -

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Basic information

Entry
Database: EMDB / ID: EMD-71069
TitleFlavobacterium johnsoniae ZAM64 mutant 70S initiation complex with rpsU mRNA containing Shine-Dalgarno sequence.
Map data
Sample
  • Complex: 70S initiation complex
    • RNA: x 5 types
    • Protein or peptide: x 49 types
KeywordsRibosome / 70S / Initiation complex / translation.
Function / homology
Function and homology information


large ribosomal subunit / ribosomal small subunit assembly / transferase activity / ribosome biogenesis / ribosomal small subunit biogenesis / 5S rRNA binding / small ribosomal subunit / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit ...large ribosomal subunit / ribosomal small subunit assembly / transferase activity / ribosome biogenesis / ribosomal small subunit biogenesis / 5S rRNA binding / small ribosomal subunit / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / RNA binding / cytoplasm / cytosol
Similarity search - Function
Protein of unknown function DUF4295 / Domain of unknown function (DUF4295) / : / Ribosomal protein L31 type B / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L31 signature. ...Protein of unknown function DUF4295 / Domain of unknown function (DUF4295) / : / Ribosomal protein L31 type B / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L16 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / : / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L28/L24 superfamily / Ribosomal protein L32p, bacterial type / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L9 / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein S13, bacterial-type / : / Ribosomal protein S19, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein L5, bacterial-type / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S4, bacterial-type / Ribosomal protein S5, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / 30S ribosomal protein S17 / : / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L14P, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S14/S29 / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L35 / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L33 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 / Ribosomal L28 family / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L33 superfamily / Ribosomal protein L28/L24 / Ribosomal protein L30, bacterial-type / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / L28p-like / Ribosomal protein L16 / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein L20 / Ribosomal protein S12, bacterial-type / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein L19 superfamily / :
Similarity search - Domain/homology
Small ribosomal subunit protein uS12 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein bL34 ...Small ribosomal subunit protein uS12 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL33 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS6 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein bL35 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL25 / DUF4295 domain-containing protein / 50S ribosomal protein L31 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL27 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL18 / AURKAIP1/COX24 domain-containing protein / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS13
Similarity search - Component
Biological speciesFlavobacterium johnsoniae (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.8 Å
AuthorsOrtega J / Arpin D
Funding support Canada, 1 items
OrganizationGrant numberCountry
Natural Sciences and Engineering Research Council (NSERC, Canada) Canada
CitationJournal: Nucleic Acids Res / Year: 2026
Title: Structure of a specialized 70S initiation complex.
Authors: Dominic Arpin / Bappaditya Roy / Fawwaz M Naeem / Kurt Fredrick / Joaquin Ortega /
Abstract: Bacteria of class Bacteroidia lack Shine-Dalgarno (SD) sequences and instead rely on other messenger RNA (mRNA) features, including upstream adenines, for start codon selection. Bacteroidia ribosomes ...Bacteria of class Bacteroidia lack Shine-Dalgarno (SD) sequences and instead rely on other messenger RNA (mRNA) features, including upstream adenines, for start codon selection. Bacteroidia ribosomes contain the anti-SD (ASD) sequence of 16S ribosomal RNA (rRNA) but are "blind" to SD sequences. This occurs due to the sequestration of the ASD through interactions with bS21, bS18, and bS6 on the 30S platform domain. In many Bacteroidia, including Flavobacterium johnsoniae, there is one gene with an extended SD-rpsU, which encodes bS21. Ribosomes lacking bS21 exhibit high-level translation of rpsU, establishing an autoregulatory circuit in the cell. In this work, we investigate the structural basis of initiation on rpsU mRNA. We find using cryo-electron microscopy that initiation entails the formation of a 13-base pair SD-ASD helix that sterically occludes bS21. Mutations of bS21, bS18, or bS6 that compromise the platform pocket liberate the 3' tail of 16S rRNA, enable SD-ASD pairing, and enhance initiation. As initiation on rpsU mRNA depends on SD-ASD pairing, we infer that dissociation of bS21 from replete ribosomes limits their initiation rate. This work shows how a compositional change of the ribosome can govern translation of a specific gene.
History
DepositionJun 6, 2025-
Header (metadata) releaseApr 15, 2026-
Map releaseApr 15, 2026-
UpdateApr 15, 2026-
Current statusApr 15, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_71069.png

Downloads & links

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Map

FileDownload / File: emd_71069.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
0.86 Å/pix.
x 480 pix.
= 410.4 Å		0.86 Å/pix.
x 480 pix.
= 410.4 Å		0.86 Å/pix.
x 480 pix.
= 410.4 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.855 Å
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Histogram (log scale)
Contour LevelBy AUTHOR: 0.08
Minimum - Maximum-0.08794608 - 0.42002964
Average (Standard dev.)0.003648049 (±0.01850258)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 410.40002 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_71069_half_map_1.map
Projections & Slices
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Half map: #1

Fileemd_71069_half_map_2.map
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Sample components

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Entire : 70S initiation complex

EntireName: 70S initiation complex
Components
  • Complex: 70S initiation complex
    • RNA: 5S rRNA
    • Protein or peptide: 50S ribosomal protein L38
    • Protein or peptide: 30S ribosomal protein S22
    • RNA: rpsU mRNA
    • RNA: fMet-tRNA
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L9
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L31
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • RNA: 23S rRNA
    • RNA: 16S rRNA

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Supramolecule #1: 70S initiation complex

SupramoleculeName: 70S initiation complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #3-#54, #1-#2
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)

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Macromolecule #1: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 927.297188 KDa
SequenceString: AAAUAAGGGC GUAUGGGGAA UGCCUAGGCU CUCAGAGGCG AUGAAGGACG UGAUAAGCUG CGAAAAGCUG CGGGGACUGG CACACACAG AUUGAUCCGC AGAUAUCCGA AUGGGGCAAC CCGCUAUAUU GAAGAUAUAG CACACCGAUA GGUGGGCAAA C CCGCUGAA ...String:
AAAUAAGGGC GUAUGGGGAA UGCCUAGGCU CUCAGAGGCG AUGAAGGACG UGAUAAGCUG CGAAAAGCUG CGGGGACUGG CACACACAG AUUGAUCCGC AGAUAUCCGA AUGGGGCAAC CCGCUAUAUU GAAGAUAUAG CACACCGAUA GGUGGGCAAA C CCGCUGAA CUGAAACAUC UAAGUAGGCG GAGGAGAAGA AAACAAAAGU GAUUCCGUAA GUAGUGGCGA GCGAACGCGG AU UAGCCCA AACCAGUGCU GUUACGGCAG UGCUGGGGUU GUAGGACCAC GACAUUUUAU GCAUGCAGAA CCGGAAGUUA CUG GAAAGU GACGCCAAAG AGGGUGAUAG CCCCGUAUGG GUAAUAAAUG UAAUAGAUAG UGGUAUCCUG AGUAGGGCGG GACA CGUGA AACCCUGUCU GAAUUCGGCG GGACCAUCCG CUAAGGCUAA AUACUCCUGA GAGACCGAUA GUGAACCAGU ACCGU GAGG GAAAGGUGAA AAGAACCGUG AAUAACGGAG UGAAAUAGAU CCUGAAACCA UACGCUUACA AGCGGUCGGA GCCCUU UCG UGGGGUGACG GCGUGCCUUU UGCAUAAUGA GCCUACGAGU UAACGCUGCU GGCAAGGAUA AGUACUUCAG GUACGGA UC CGCAGCGAAA GCGAGUCUGA AUAGGGCGCU UUAGUCAGUA GUGUUAGACG CGAAACCGUG UGAUCUACCC AUGGGCAG G UUGAAGCUGU GGUAACACAC AGUGGAGGAC CGAACCGGUU GACGUUGAAA AGUCUUCGGA UGACCUGUGG GUAGGGGUG AAAGGCCAAU CAAACUCGGA AAUAGCUCGU ACUCCCCGAA AUGCAUUUAG GUGCAGCGUU AGUUAUAAAG UUAUACAGAG GUAGAGCUA CUGAUUGGAU GCGGGGGCUU CACCGCCCAC CAAUUCCUGA CAAACUCCGA AUGCUGUAUA AUGUUCACUA A CAGUGAGG GCUUGGGUGC UAAGGUCCAA GUCCGAGAGG GAAAGAACCC AGACCAUCAG CUAAGGUCCC CAAAUAUAUG CU AAGUUGA AAGAACGAGG UUUGUCUGCC CAGACAGCUA GGAUGUUGGC UUGGAAGCAG CCAUUCAUUU AAAGAGUGCG UAA CAGCUC ACUAGUCGAG CGGACGAGCA UGGAUAAUAA UCGGGCAUAA GCAUAUUACC GAAGCUAUGG AUUUGCAAGU AAUU GCAAG UGGUAGGGGA GCAUUCUAAC AGGGUUGAAG GUUUUUCGUA AGGAGGGCUG GACUGGUUAG AAAAGAAAAU GUAGG CAUA AGUAACGAUA AUGCGGGCGA GAAACCCGCA CACCGAAAAA CUAAGGUUUC CACAGCUAUG CUAAUCAGCU GUGGGU UAG UCUGGUCCUA AGGCGAACCC GAAAGGGACA GUCGAUGGCU AACGGGUUAA UAUUCCCGUA CUACUAAUUA CUGUGAU GG GGUGACGGAG UGAUGAAAGC GCCGCGAACU GACGGAAUAG UUCGUUGAAG UACCUAGCUA UAGGUCCUGU AGUAAAAU G CGCAGGAACU GGUGAAAUAC GAUAGUACUC GGAGUCUUCG GACAAAGAGA UAGUGCGCCU AAGGGCUUCC AAGAAAAAC CUCUAAACUU CAGGUAAUUA GUACCAGUAC CGUAAACCGA CACAGGUAGU UGAGGAGAGA AUCCUAAGGU GCUCGAGAUA UUCAUGGCU AAGGAAUUAG GCAAAAUAGA CCUGUAACUU CGGGAGAAAG GUCGCCCUGA GCAAUCAGGG CCGCAGUGAA G AGGUCCAG GCGACUGUUU AUCAAAAACA CAGGGCUCUG CAAAAUCGUA AGAUGAAGUA UAGGGCCUGA CACCUGCCCG GU GCUGGAA GGUUAAGAGG AGAUGUUAUC UUCGGAGAAG CAUUGAAUUG AAGCCCCAGU AAACGGCGGC CGUAACUAUA ACG GUCCUA AGGUAGCGAA AUUCCUUGUC GGGUAAGUUC CGACCUGCAC GAAUGGUGUA ACGAUCUGGA CACUGUCUCA GCCA UGAGC UCGGUGAAAU UGUAGUAACG GUGAAGAUGC CGUUUACCCG CAGUGGGACG AAAAGACCCU GUGCACCUUU ACUAU AGCU UAGUACUGAC CUUGGAUAAA UGAUGUGUAG GAUAGGUUGG AGACUAUGAA GCGGCGUCGC CAGGCGUUGU GGAGUC AUU GUUGAAAUAC AACCCUUUGU UUAUCUGAGG CCUAACCUCG CUAUGCGGGG GACAGUGCUU GGUGGGUAGU UUGACUG GG GUGGUCGCCU CCAAAAGAGU AACGGAGGCU UCUAAAGGUU CCCUCAGUAC GCUUGGUAAC CGUGCGUAGA GUGCAAUG G CAUAAGGGAG CUUGACUGAG AGACAUACAG GUCGAUCAGG UACGAAAGUA GAGCAUAGUG AUCCGGUGGU UCCGCAUGG AAGGGCCAUC GCUCAAAGGA UAAAAGGUAC GCCGGGGAUA ACAGGCUGAU CUCCCCCAAG AGUUCAUAUC GACGGGGGGG UUUGGCACC UCGAUGUCGG CUCGUCACAU CCUGGGGCUG GAGAAGGUCC CAAGGGUUGG GCUGUUCGCC CAUUAAAGUG G UACGCGAG CUGGGUUCAG AACGUCGUGA GACAGUUCGG UCUCUAUCUA CUGCGGGCGU UAGAAAUUUG AGUGGAUCUG AU UCUAGUA CGAGAGGACC GAAUUGGACU AACCUCUAGU GUAUCUGUUG UCCCGCCAGG GGCACCGCAG AGUAGCUACG UUG GGAAGG GAUAAGCGCU GAAAGCAUAU AAGCGCGAAA CCCACCACAA GAUGAGAUUU CUUUUAAGGA UCGUGGAAGA UGAC CACGU UGAUAGGCUA UAGAUGUAAA GGCAGUAAUG UCAUAGUCGA GUAGUACUAA UAAUCCG

GENBANK: GENBANK: CP031763.1

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Macromolecule #2: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 491.83325 KDa
SequenceString: UAUACGAUGA AGAGUUUGAU CCUGGCUCAG GAUGAACGCU AGCGGCAGGC UUAACACAUG CAAGUCGAGG GGUAGAAGUC UUCGGGCUU UGAGACCGGC GCACGGGUGC GUAACGCGUA UGCAAUCUGC CUUUCACAGA GGGAUAGCCC AGAGAAAUUU G GAUUAAUA ...String:
UAUACGAUGA AGAGUUUGAU CCUGGCUCAG GAUGAACGCU AGCGGCAGGC UUAACACAUG CAAGUCGAGG GGUAGAAGUC UUCGGGCUU UGAGACCGGC GCACGGGUGC GUAACGCGUA UGCAAUCUGC CUUUCACAGA GGGAUAGCCC AGAGAAAUUU G GAUUAAUA CCUCAUAGUA UUAUAGAGCG GCAUCACUUU AUAAUUAAAG UCACAACGGU GAAAGAUGAG CAUGCGUCCC AU UAGCUAG UUGGUAAGGU AACGGCUUAC CAAGGCCACG AUGGGUAGGG GUCCUGAGAG GGAGAUCCCC CACACUGGUA CUG AGACAC GGACCAGACU CCUACGGGAG GCAGCAGUGA GGAAUAUUGG UCAAUGGACG CAAGUCUGAA CCAGCCAUGC CGCG UGCAG GAUGACGGUC CUAUGGAUUG UAAACUGCUU UUGUACGAGA AGAAACACUC CUAUAUAUAG GAGCUUGACG GUAUC GUAA GAAUAAGGAU CGGCUAACUC CGUGCCAGCA GCCGCGGUAA UACGGAGGAU CCAAGCGUUA UCCGGAAUCA CUGGGU UUA AAGGGUCCGU AGGCGGUUUA GUAAGUCAGU GGUGAAAGCC CAUCGCUCAA CGGUGGAACG GCCAUUGAUA CUGCUGA AC UUGAAUUACU GGGAAGUAAC UAGAAUAUGU AGUGUAGCGG UGAAAUGCUU AGAUAUUACA UGGAAUACCA AUUGCGAA G GCAGGUUACU ACCAGUUUAU UGACGCUGAU GGACGAAAGC GUGGGGAGCG AACAGGAUUA GAUACCCUGG UAGUCCACG CCGUAAACGA UGGAUACUAG CUGUUGGGCG CAAGUUCAGU GGCUAAGCGA AAGUGAUAAG UAUCCCACCU GGGGAGUACG AACGCAAGU UUGAAACUCA AAGGAAUUGA CGGGGGCCCG CACAAGCGGU GGAACAUGUG GUUUAAUUCG AUGAUACGCG A GGAACCUU ACCAAGGCUU AAAUGCAGAC UGACCGAUUU GGAAACAGAU CUUAUCGCAA GACAGUUUAC AAGGUGCUGC AU GGUUGUC GUCAGCUCGU GCCGUGAGGU GUCAGGUUAA GUCCUAUAAC GAGCGCAACC CCUGUUGUUA GUUGCCAGCG AGU GAUGUC GGGAACUCUA ACAAGACUGC CAGUGCAAAC UGUGAGGAAG GUGGGGAUGA CGUCAAAUCA UCACGGCCCU UACG CCUUG GGCUACACAC GUGCUACAAU GGCCGGUACA GAGAGCAGCC ACUGGGUGAC CAGGAGCGAA UCUAUAAAGC CGGUC ACAG UUCGGAUCGG AGUCUGCAAC UCGACUCCGU GAAGCUGGAA UCGCUAGUAA UCGGAUAUCA GCCAUGAUCC GGUGAA UAC GUUCCCGGGC CUUGUACACA CCGCCCGUCA AGCCAUGGAA GCUGGGGGUG CCUGAAGUCG GUGACCGCAA GGAGCUG CC UAGGGUAAAA CUGGUAACUA GGGCUAAGUC GUAACAAGGU AGCCGUACCG GAAGGUGCGG CUGGAACACC UCCUUUCU

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Macromolecule #3: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 35.661992 KDa
SequenceString:
UUAAGGUGGU UAUUGCGGCG GGGCUCACCU CUUCCCAUCC CGAACAGAGA AGUUAAGCCC GCUUGCGCAG AUGGUACUGC AGUUUUGUG GGAGAGUAUG UCGUCGCCUU UC

GENBANK: GENBANK: CP000685.1

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Macromolecule #6: rpsU mRNA

MacromoleculeName: rpsU mRNA / type: rna / ID: 6 / Number of copies: 1
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 7.445449 KDa
SequenceString:
CGAGAGGAGG UGUCUAUAUU AUG

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Macromolecule #7: fMet-tRNA

MacromoleculeName: fMet-tRNA / type: rna / ID: 7 / Number of copies: 1
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 24.786785 KDa
SequenceString:
CGCGGGGUGG AGCAGCCUGG UAGCUCGUCG GGCUCAUAAC CCGAAGAUCG UCGGUUCAAA UCCGGCCCCC GCAACCA

GENBANK: GENBANK: CP122315.1

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Macromolecule #4: 50S ribosomal protein L38

MacromoleculeName: 50S ribosomal protein L38 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 5.569749 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MAKKTVASLQ TSSKRLSKAI KMVKSPKTGA YIFVESIMAP ELVDEFLKKK

UniProtKB: DUF4295 domain-containing protein

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Macromolecule #5: 30S ribosomal protein S22

MacromoleculeName: 30S ribosomal protein S22 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 3.696608 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MPSGKKRKRH KVATHKRKKR ARANRHKKKK

UniProtKB: AURKAIP1/COX24 domain-containing protein

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Macromolecule #8: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 29.991619 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString: MSVRKLKPIT PGQRFRVVNG YDAITTDKPE RSLISPIKNS GGRNSQGKMT MRYTGGGHKQ RYRIIDFKRT KDGIPATVKS IEYDPNRTA FIALLAYADG EKTYIIAQNG LKVGQKLVSG PESQPEIGNT LPLSRIPLGT VISCIELRPG QGAVIARSAG T FAQLMARD ...String:
MSVRKLKPIT PGQRFRVVNG YDAITTDKPE RSLISPIKNS GGRNSQGKMT MRYTGGGHKQ RYRIIDFKRT KDGIPATVKS IEYDPNRTA FIALLAYADG EKTYIIAQNG LKVGQKLVSG PESQPEIGNT LPLSRIPLGT VISCIELRPG QGAVIARSAG T FAQLMARD GKYATIKMPS GETRLILLTC SATIGEVSNS DHQLVVSGKA GRTRWLGRRP RTRPVAMNPV DHPMGGGEGR SS GGHPRSR NGLPAKGYRT RSKKNPSNKY IVERRKK

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #9: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 21.975359 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString: MSGLIGKKIG MTSIFDENGK NIPCTVIEAG PCVVTQVRTN EVDGYEALQL GFDDKNEKHS TKAALGHFKK AGTVAKKKVV EFQDFAAAQ ALGDLIDVSI FEEGEFVDVQ GVSKGKGFQG VVKRHGFGGV GQATHGQHQR LRAPGSVGAS SYPSRVFKGM R MAGRMGGD ...String:
MSGLIGKKIG MTSIFDENGK NIPCTVIEAG PCVVTQVRTN EVDGYEALQL GFDDKNEKHS TKAALGHFKK AGTVAKKKVV EFQDFAAAQ ALGDLIDVSI FEEGEFVDVQ GVSKGKGFQG VVKRHGFGGV GQATHGQHQR LRAPGSVGAS SYPSRVFKGM R MAGRMGGD NVKVQNLRVL KVVAEKNLLV VKGCIPGHKN SYVIIQK

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #10: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 23.187557 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString: MEVKVLDFNG KDTGRKVQLS DSVFAIEPNN HAVYLDVKQY LANQRQGTHK AKERAEVTGS TRKIKKQKGT GTARAGSVKN PLFKGGGTV FGPRPRSYSF KLNKNLKRLA RKSAFSIKAK ESNIIVLEDF NFEAPNTKNF INVLKALGLE NKKSLFVLGE S NKNVYLSS ...String:
MEVKVLDFNG KDTGRKVQLS DSVFAIEPNN HAVYLDVKQY LANQRQGTHK AKERAEVTGS TRKIKKQKGT GTARAGSVKN PLFKGGGTV FGPRPRSYSF KLNKNLKRLA RKSAFSIKAK ESNIIVLEDF NFEAPNTKNF INVLKALGLE NKKSLFVLGE S NKNVYLSS RNLKASNVVT SSELSTYAIL NTNNLVLLEG SLELIEENLS K

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #11: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 20.79932 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MAYTPRLKEE YKSRVISALK EEFGYTNVMQ VPKLEKIVLS RGVGAAVSDK KLIDYAVDEL TKITGQKAVI TKARKSVAGF KIRQGYPIG CKVTLRGERM WEFFERLITI AVPRIRDFRG LSAKSFDGRG NYSMGVREQI IFPEIDYDKV DRVRGMDITF V TTAKTDKE AKSLLAELGL PFKKN

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Macromolecule #12: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 19.420445 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MSRIGKSPIV IPAGVTVEVK DGIITVKGKK GQLVQEFSDV NVTVEGDQVL VERSSDHKDH RAKHGLFRSL ISNMVVGVSE GFTKELELV GVGYRAANQG NKLDLALGYS HNIVLEIAPE VSLETISEAG ANPIVKLTSF DKQLLGQVAA KIRGFRKPEP Y KGKGVKFV GEVLRRKAGK SA

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #13: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 15.968622 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MEIILKQDVQ NLGFKDDVVS VKPGYGRNFL IPQGFATLAT PSAKKVLAEN LKQRAHKEAK IVADAKALAE TLKALEIKLT AKAGGEKLF GSITNIDIAE ALEKSGNAID RKFITSGVVK RIGKYNATVR LHRDVIVELP YEIVAEK

UniProtKB: Large ribosomal subunit protein bL9

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Macromolecule #14: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 16.578273 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MDALSYKTIS ASKATVTKEW IVVDAEGHNL GRLASKVAMI LRGKYKPSYT PHVDCGDNVI VINSEKINLT GTKLNDKIYM RHTGYPGGQ RTLTAKVLQS KNPALLVEKA VKGMLPKNKL GAELFRNLNV VVGSEHTHGA QKPRTVNLND LK

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #15: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 13.339537 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MVQQESRLKV ADNTGAKEVL TIRVLGGTKR RYASVGDKIV VSIKDATPNG NVKKGAVSTA VVVRTKKEVR RADGSYIRFD DNACVLLNA AGEMRGTRVF GPVARELREK QFMKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #16: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 15.994265 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MNLSNLQPAE GSTHNQNKRV GRGEGSGKGG TAARGHKGAK SRSGYSKKIG FEGGQMPLQR RVPKFGFKNI NRKEYEGVNL DTLQLLVDN GVITDSVSMT DFVANRLASK NEIVKILGRG ELKAKLKVTA HKFTATAKAA IEAAGGEAVT I

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #17: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 16.075122 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MLQPKRTKYR KVQKGRMKGN SQRGHELSNG MFGIKSVHED GMFLTSRQIE AARIAATRYM KREGQLWIKI FPDKPITKKP LEVRMGKGK GAVEYWWAVV KPGRIMFEVG GVPLSVAKEA LRLAAQKLPV KTKFVVARDF EA

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #18: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 18.092869 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MRHGKKINHL SRQTGHRKAM LANMACSLIE HKRINTTVAK AKALKQFVEP LITKSKEDTT HNRRIVFAYL RSKYAVTDLF RDVAAKVGD RPGGYTRIIK VGNRLGDNAD MAMIELVDFN ELYNGGKKEV KKAKSRRGGK AKKAEGTAPE APAAESESTT E ASE

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #19: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 12.66072 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MSLTKSERRQ RIKFRIRKTI SGSAAKPRLS VFRSNKEIYA QLIDDVNGVT ILAASSREKE IAKGTNVEIA AAVGKLVAEK ALKAGIDTI TFDRGGYLYH GRIKSLAEGA RAAGLKF

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #20: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 13.316622 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MADLLKFVQN EFVAKKDFPD FGAGDTITVF YEIKEGEKTR TQFFKGVVIQ RRGSGTTETF TIRKMSGAIG VERIFPVNLP ALQKIEINK KGAVRRARIF YFRELTGKKA KIKDKLR

UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #21: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 13.244699 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MPRSVNSVAK RARRKKIMKQ AKGFFGRRKN VWTVAKNAVE KAMSYAYRDR KQNKRNFRSL WIQRINAGAR LEGMSYSQFM GKVKANGIE LNRKVLADLA MNHPEAFKAI LNKVK

UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #22: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 15.90152 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MYAIVEIAGQ QFKVSKDLKV YVHRLANEEG SKVSFDKVLL LDDNGNVTLG APAIEGASVE AKVLQHLKGD KVIVFKKKRR KGYKKRNGH RQYLTQIVIE GITAAGGTKK AAAKKAVVAE EAAATEEVEA APKAKKAAPK AKKEATKE

UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #23: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 15.475993 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MGVRKRETAD ARKEANKSIA FAKLNNCPTS PRKMRLVADL VRGQKVERAL NILRFSSKEA SRKLEKLLLS AINNWEQKNS EGNLEEAGL FVKEIRVDGG MMLKRLRPAP QGRAHRIRKR SNHVTIVLGA INNTQSNS

UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #24: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 10.690438 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MSIIIRPIVT EKVTKESEVL NRFGFVVDKK ANKVQIKKAV EAAYGVTIVS VNTMNVRPDR STKYTKSGLI SGKTNAIKKA IVQVQEGET IDFYNNI

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #25: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 11.439517 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MIKLKIKSGD IVRVIAGDHK GAEGKVLRVY REKNKAIVEG VNLVSKHTKP SAKNPQGGIV KKEASIQISN IALIDPKTKE TTRVGIRVE GDKKVRFSKK SNQVL

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #26: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 21.89165 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString: MKSITIKGSE RESVGKVSTK ALRNAGAVPC VLYGGNQAVH FSADAAAFKN LVYTPNAHTV VIELGAGKSF NAILQDIQVH PVTDKILHI DFFQLFDDKE ITMEVPVKIV GTSKGVLAGG VLRLNQRKLK VKALPANIPE FVEADITPLE MGNKLYVTKV G KPEYKIMH ...String:
MKSITIKGSE RESVGKVSTK ALRNAGAVPC VLYGGNQAVH FSADAAAFKN LVYTPNAHTV VIELGAGKSF NAILQDIQVH PVTDKILHI DFFQLFDDKE ITMEVPVKIV GTSKGVLAGG VLRLNQRKLK VKALPANIPE FVEADITPLE MGNKLYVTKV G KPEYKIMH PDNTVVAQVR ISRAAMKAAQ EAAKAAKAPA KGKKK

UniProtKB: Large ribosomal subunit protein bL25

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Macromolecule #27: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 9.422824 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MAHKKGVGSS KNGRESESKR LGVKIYGGQA AIAGNIIVRQ RGSKHNPGEN VYISKDHTLH ARVAGVVKFQ KKRDNKSYVS IIPFEA

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #28: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 8.942626 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MSRVCDLTGK RAMVGNNVSH AMNKTKRKFS VNLVKKRFYL PEEDRWITLR VQRSTIKTIN KNGITAVLKK AQSAGFIK

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #29: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 7.189451 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MKQSEIKDLS AAELQEKLSQ TKKVYADLKM AHAISPIANP LRIRSVRRTV ARLATELTKR ELQ

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #30: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 6.643993 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MAKLLVKQVR SKINCPLSQK RGLEALGLRK LGQVVEHESN PAILGMINKV KHLVSVEEAK

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #31: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 9.646128 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MKKGIHPENY RLVAFKDMSN DDVFITKSTA DTKETIEVDG VEYPVVKMEI SRTSHPFYTG KSKLIDTAGR IDKFKTKYAK HAKK

UniProtKB: 50S ribosomal protein L31

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Macromolecule #32: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 7.518691 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MAHPKRKTSK TRRDKRRTHY KATVAQIATC PITGEAHLYH RAYWHEGKMY YRGQVVIDKS EAVA

UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #33: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 6.935232 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MAKKGNRIQV ILECTEHKTS GVPGTSRYIT TKNKKNTPDR LEIKKFNPIL KRVTVHKEIK

UniProtKB: Large ribosomal subunit protein bL33

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Macromolecule #34: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 6.324544 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MSKRTFQPSK RKRRNKHGFM DRMASANGRK VLARRRAKGR HKLTVSSEPR HKK

UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #35: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 7.522242 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MPKMKTKSSA KKRFKVTGSG KIKRKHAFKS HILTKKSKKR KLALTHSALV HKTDEKSIKQ QLRII

UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #36: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 4.557577 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MKVRASVKKR SAECIIVRRK GRLYVINKKN PRFKQRQG

UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #37: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 27.886365 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString: MGQKTNPIGN RLGIIRGWDS NWYGGNDYGD KLAEDHKIRK YIHARLSKAS VSKVIIERTL KLVTVTITTA RPGIIIGKGG QEVDKLKEE LKKVTDKEVQ INIFEIKRPE LDAYLVATSI ARQIESRISY RRAIKMAIAA SMRMNAEGIK VLISGRLNGA E MARSEGFK ...String:
MGQKTNPIGN RLGIIRGWDS NWYGGNDYGD KLAEDHKIRK YIHARLSKAS VSKVIIERTL KLVTVTITTA RPGIIIGKGG QEVDKLKEE LKKVTDKEVQ INIFEIKRPE LDAYLVATSI ARQIESRISY RRAIKMAIAA SMRMNAEGIK VLISGRLNGA E MARSEGFK EGRIPLSTFR ADIDYALAEA HTTYGRMGIK VWIMKGEVYG KRDLSPLAGM DKKQSGTGGG KGGDAPRGKS NF NKGGKPD ARKRK

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #38: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 22.950443 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString: MARYTGPKTK IARKFGEAIF GDDKSFEKRN YPPGQHGMAK KRGKKSEYAV QLMEKQKAKY SYGILEKQFR NLFEKASATK GVTGEVLLQ LCEARLDNVV FRMGIAPSRR GARQIVSHRH ITVNGEVVNI PSYHLKPGDK VAVREKSKSL EAIERSLSNS S HVYEWITW ...String:
MARYTGPKTK IARKFGEAIF GDDKSFEKRN YPPGQHGMAK KRGKKSEYAV QLMEKQKAKY SYGILEKQFR NLFEKASATK GVTGEVLLQ LCEARLDNVV FRMGIAPSRR GARQIVSHRH ITVNGEVVNI PSYHLKPGDK VAVREKSKSL EAIERSLSNS S HVYEWITW NNDLKEGTFV SVPARLQIPE NIKEQLIVEL YNK

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #39: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 18.20602 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MSKYKNVELV KPSGLELKDR LVSVNRVTKV TKGGRAFGFS AIVVVGDENG VVGHGLGKSK DVSEAIAKAV EDAKKNLVRI PLNGQSVPH EQKGKFGGAR VFLIPASHGT GVIAGGAVRS VLESVGIHDV LSKSQGSSNP HNVVKATFDA LLQMRSAHTV A KQRGVSLE KVFKG

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #40: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 13.376448 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MNHYETVFIL NPVLSEVQVK ETVTKFEEFL TSRGAEMVSK EDWGLKKMAY EIQNKKSGFY HLFEFKVAGE VLIAFETEFR RDERVMRFL TVSLDKHAIS WAERRRAKLK STKA

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #41: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 18.493707 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MRKRAAKKRP LLPDPRFNDQ LVTRFVNNLM WDGKKSTAFK VFYDAIDIIE TKKQNDEKTS LEIWKDALTN VMPHVEVRSR RVGGATFQI PMQIRPDRKI SMAMKWLILY ARRRNEKSMA QRLASECLAA AKEEGAAVKK RMDTHKMAEA NKAFSHFRF

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #42: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 14.652163 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MYTDPIADYL TRVRNAVAAN HKVVEIPASN LKKEITKILF DQGYILSYKF EQNTVQGSIK IALKYDKDTK EPVIKDIQRI SKPGLRKYA GAAKLPRILN GLGIAIVSTS KGLMTGKQAK QLNVGGEVIC YVY

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #43: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 14.464888 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MGVIHKIGRR KTAVARVYVS EGTGNITVNK KEFATYFPTA TLQYKVLQPL SMTENVNNFD VKVNVYGGGT TGQAEAVRMA LARVMCEVN AENRGILKPE GLLTRDPRMV ERKKFGQKKA RKRFQFSKR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #44: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 11.44664 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MSQKIRIKLK SYDHMLVDKS AEKIVKTVKT TGAVVTGPIP LPTHKKLFTV LRSPHVNKKA REQFEVMSYK RLIDIYSSSS KTIDALMKL ELPSGVEVEI KV

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #45: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 13.700957 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MAKATAKKRK VIVESTGEAH ISSTFNNIII SLTNKKGEVI AWSSAGKMGF RGSKKNTPYA AQMAAEDCSK VALEAGLKKV KVYVKGPGN GRESAIRSIH NGGIEVTEII DVTPMPHNGC RPPKRRRV

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #46: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 14.121425 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MPTIQQLVRT GRTQITKKSK SVALDSCPQR RGVCTRVYTT TPKKPNSAMR KVARVRLTNG NEVNAYIPGE GHNLQEHSIV LVRGGRVKD LPGVRYHIVR GALDTSGVAG RTQRRSKYGA KRPKEAKK

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #47: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 13.734152 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MARIAGINIP DHKHAVIALT SIYGVGKTRS KAILAAAGIA EDVKISELSE GQIDTLRDEV AKFVVEGDLR REISMSIKRL MDLGCYRGL RHRRGLPVRG QRTKTNARTR KGPRKTVANK KKATK

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #48: 30S ribosomal protein S14

MacromoleculeName: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 10.211011 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MAKESMKARE VKREKTVAKY AEKRKALLEA GDYEGLQRLP KNASPVRLHN RCKLTGRPRG YIRQFGISRV TFREMANNGL IPGVKKASW

UniProtKB: Small ribosomal subunit protein uS14

+
Macromolecule #49: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 10.565339 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MYLTKEKKEE IFAQHGDAKN TGKAEGQIAL FTYRISHLTE HLKKNRHDYN TERSLVLLVG KRRALLDYLK RKDINRYREI IKVLNIRK

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #50: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 20.106588 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MSVKIRLQRH GKKGKPFYWV VAADARSKRD GKYLEKIGTY NPNTNPATVE LNLDSIVKWL HQGAQPTDTA RAILSYKGAL LKHHLDGGV RKGALTQEQA DAKLTAWLEA KAGKVDAKKD GLSKAQADAK AKALKAEKEV NDKRIAAAAK AEADAIAAAS A TEEAATEE VAEAAEEAPA AEENNETTEA

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #51: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 10.1089 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MEEKRNLRKE RIGVVTSNKM DKSIVISEVR KVKHPLYGKF VLKTKKYVAH DETNDCNIGD TVRISETRPL SKTKCWRLVE ILERAK

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #52: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 11.378475 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MSTIEQSAKG KKDGDIRYLT PLNIETNKTK KYCRFKKSGI KYIDYKDADF LLKFVNEQGK ILPRRLTGTS LKYQRKVSVA VKRARHLAL MPYVADLLK

UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #53: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 10.278883 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MARSLKKGPF VHYKLDKKVQ ENVESGKNSV VKTWSRASMI TPDFVGQTIA VHNGRQFVPV YVTENMVGHK LGEFSPTRSF RGHAGAKNK GKK

UniProtKB: Small ribosomal subunit protein uS19

+
Macromolecule #54: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Flavobacterium johnsoniae (bacteria)
Molecular weightTheoretical: 9.442211 KDa
Recombinant expressionOrganism: Flavobacterium johnsoniae (bacteria)
SequenceString:
MANHKSALKR IRSNEKRRVL NRYQHKTTRN AIKALRLATD KSDAAAKLST VISMIDKLAK KNIIHDNKAS NLKSKLTKHV AKL

UniProtKB: Small ribosomal subunit protein bS20

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 249488
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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