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- EMDB-70885: Structure of hTWIK2RM (K2P6.1RM) ion channel with lipid plug in D... -

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Basic information

Entry
Database: EMDB / ID: EMD-70885
TitleStructure of hTWIK2RM (K2P6.1RM) ion channel with lipid plug in DDM detergent
Map data
Sample
  • Complex: Human TWIK2RM (K2P6.1RM)
    • Protein or peptide: Potassium channel subfamily K member 6
  • Ligand: POTASSIUM ION
  • Ligand: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
Keywordsion channel / K2P / TWIK2 / two pore channel / potassium channel / membrane protein / K2P6.1 / lipid plug / TWIK2RM
Function / homology
Function and homology information


regulation of lysosome size / Tandem of pore domain in a weak inwardly rectifying K+ channels (TWIK) / Phase 4 - resting membrane potential / potassium ion leak channel activity / regulation of resting membrane potential / inward rectifier potassium channel activity / outward rectifier potassium channel activity / negative regulation of systemic arterial blood pressure / positive regulation of NLRP3 inflammasome complex assembly / potassium channel activity ...regulation of lysosome size / Tandem of pore domain in a weak inwardly rectifying K+ channels (TWIK) / Phase 4 - resting membrane potential / potassium ion leak channel activity / regulation of resting membrane potential / inward rectifier potassium channel activity / outward rectifier potassium channel activity / negative regulation of systemic arterial blood pressure / positive regulation of NLRP3 inflammasome complex assembly / potassium channel activity / voltage-gated potassium channel complex / potassium ion transmembrane transport / potassium ion transport / late endosome membrane / lysosomal membrane / metal ion binding / plasma membrane
Similarity search - Function
Two pore domain potassium channel, TWIK-2 / Two pore domain potassium channel, TWIK family / Two pore domain potassium channel, TASK family / Two pore domain potassium channel / Potassium channel domain / Ion channel
Similarity search - Domain/homology
Potassium channel subfamily K member 6
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.9 Å
AuthorsMondal A / Minor DL
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of Mental Health (NIH/NIMH)R01 MH093603 United States
CitationJournal: To Be Published
Title: Structure of hTWIK2RM (K2P6.1RM) ion channel with lipid plug in DDM detergent
Authors: Mondal A / Minor D
History
DepositionMay 29, 2025-
Header (metadata) releaseMay 27, 2026-
Map releaseMay 27, 2026-
UpdateMay 27, 2026-
Current statusMay 27, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_70885.png

Downloads & links

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Map

FileDownload / File: emd_70885.map.gz / Format: CCP4 / Size: 67 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.82 Å/pix.
x 260 pix.
= 213.2 Å		0.82 Å/pix.
x 260 pix.
= 213.2 Å		0.82 Å/pix.
x 260 pix.
= 213.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.82 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.14
Minimum - Maximum-1.8805652 - 2.225746
Average (Standard dev.)0.0029036328 (±0.049314044)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions260260260
Spacing260260260
CellA=B=C: 213.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_70885_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_70885_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Human TWIK2RM (K2P6.1RM)

EntireName: Human TWIK2RM (K2P6.1RM)
Components
  • Complex: Human TWIK2RM (K2P6.1RM)
    • Protein or peptide: Potassium channel subfamily K member 6
  • Ligand: POTASSIUM ION
  • Ligand: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine

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Supramolecule #1: Human TWIK2RM (K2P6.1RM)

SupramoleculeName: Human TWIK2RM (K2P6.1RM) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 / Details: Mutation in ER retention signal
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Potassium channel subfamily K member 6

MacromoleculeName: Potassium channel subfamily K member 6 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.191209 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: GALLAGALAA YAAYLVLGAL LVARLEGPHE ARLRAELETL RAQLLQRSPC VAAPALDAFV ERVLAAGRLG RVVLANASGS ANASDPAWD FASALFFAST LITTVGYGYT TPLTDAGKAF SIAFALLGVP TTMLLLTASA QRLSLLLTHV PLSWLSMRWG W DPRRAACW ...String:
GALLAGALAA YAAYLVLGAL LVARLEGPHE ARLRAELETL RAQLLQRSPC VAAPALDAFV ERVLAAGRLG RVVLANASGS ANASDPAWD FASALFFAST LITTVGYGYT TPLTDAGKAF SIAFALLGVP TTMLLLTASA QRLSLLLTHV PLSWLSMRWG W DPRRAACW HLVALLGVVV TVCFLVPAVI FAHLEEAWSF LDAFYFCFIS LSTIGLGDYV PGEAPGQPYR ALYKVLVTVY LF LGLVAMV LVLQTFRHVS DLHGLTELIL LPPPC

UniProtKB: Potassium channel subfamily K member 6

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Macromolecule #2: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 2 / Number of copies: 3 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #3: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine

MacromoleculeName: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine / type: ligand / ID: 3 / Number of copies: 2 / Formula: LBN
Molecular weightTheoretical: 760.076 Da
Chemical component information

ChemComp-LBN:
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine / phospholipid*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 47.7 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.1 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

8ue2

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.6.0) / Number images used: 112432
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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