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- EMDB-70632: Focused map of PCMTD1-ELOBC: Cryo-EM structure of PCMTD1-ELOBC-CU... -

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Basic information

Entry
Database: EMDB / ID: EMD-70632
TitleFocused map of PCMTD1-ELOBC: Cryo-EM structure of PCMTD1-ELOBC-CUL5-RBX2 (CRL5-PCMTD1)
Map dataFocused map of PCMTD1-ELOBC within CRL5-PCMTD1 sharpened via EMReady
Sample
  • Complex: Pentameric complex of PCMTD1, Elongins B and C, Cullin-5, and RING-box protein 2 (CRL5-PCMTD1)
    • Complex: PCMTD1-ELOBC substrate receptor complex
      • Complex: PCMTD1
    • Complex: CUL5-RBX2 catalytic core
KeywordsCUL5-RING ubiquitin ligase complex / PROTEIN BINDING
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.18 Å
AuthorsPang EZ / Zhao B / Flowers C / Oroudjeva E / Winters JB / Pandey V / Sawaya MR / Wohlschlegel W / Loo JA / Rodriguez JA / Clarke SG
Funding support United States, 8 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)T32GM136614 United States
National Science Foundation (NSF, United States)MCB-1714569 United States
National Science Foundation (NSF, United States)DMR-1548924 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM128867 United States
National Institutes of Health/National Center for Research Resources (NIH/NCRR)S10RR028893 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM145286 United States
Department of Energy (DOE, United States)DE-FC02-02ER63421 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)T32GM145388 United States
CitationJournal: Biorxiv / Year: 2025
Title: Structural basis for L-isoaspartyl-containing protein recognition by the PCMTD1 cullin-RING E3 ubiquitin ligase
Authors: Pang EZ / Zhao B / Flowers C / Oroudjeva E / Winter JB / Pandey V / Sawaya MR / Wohlschlegel J / Loo JA / Rodriguez JA / Clarke SG
History
DepositionMay 14, 2025-
Header (metadata) releaseJun 4, 2025-
Map releaseJun 4, 2025-
UpdateJun 4, 2025-
Current statusJun 4, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_70632.png

Downloads & links

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Map

FileDownload / File: emd_70632.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationFocused map of PCMTD1-ELOBC within CRL5-PCMTD1 sharpened via EMReady
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.86 Å/pix.
x 480 pix.
= 412.8 Å		0.86 Å/pix.
x 480 pix.
= 412.8 Å		0.86 Å/pix.
x 480 pix.
= 412.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.86 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 3.0
Minimum - Maximum-0.1438286 - 13.267563000000001
Average (Standard dev.)-0.029698752 (±0.1582801)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 412.80002 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_70632_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Unsharpened focused map of PCMTD1-ELOBC generated in cryoSPARC

Fileemd_70632_additional_1.map
AnnotationUnsharpened focused map of PCMTD1-ELOBC generated in cryoSPARC
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_70632_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_70632_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Pentameric complex of PCMTD1, Elongins B and C, Cullin-5, and RIN...

EntireName: Pentameric complex of PCMTD1, Elongins B and C, Cullin-5, and RING-box protein 2 (CRL5-PCMTD1)
Components
  • Complex: Pentameric complex of PCMTD1, Elongins B and C, Cullin-5, and RING-box protein 2 (CRL5-PCMTD1)
    • Complex: PCMTD1-ELOBC substrate receptor complex
      • Complex: PCMTD1
    • Complex: CUL5-RBX2 catalytic core

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Supramolecule #1: Pentameric complex of PCMTD1, Elongins B and C, Cullin-5, and RIN...

SupramoleculeName: Pentameric complex of PCMTD1, Elongins B and C, Cullin-5, and RING-box protein 2 (CRL5-PCMTD1)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#5
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #2: PCMTD1-ELOBC substrate receptor complex

SupramoleculeName: PCMTD1-ELOBC substrate receptor complex / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1, #4-#5
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #3: PCMTD1

SupramoleculeName: PCMTD1 / type: complex / ID: 3 / Parent: 2 / Macromolecule list: #1
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #4: CUL5-RBX2 catalytic core

SupramoleculeName: CUL5-RBX2 catalytic core / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #2-#3
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration2.8 mg/mL
BufferpH: 7.4
Component:
ConcentrationName
50.0 mMHEPES
150.0 mMsodium chloride
1.0 mMDTT
GridModel: SPT Labtech self-wicking R1.2/0.8 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Instrument: SPT LABTECH CHAMELEON
DetailsSample was monodisperse and freshly gel-filtrated prior to sample vitrification

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number real images: 5447 / Average exposure time: 2.1 sec. / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 1302046
CTF correctionType: NONE
Startup modelType of model: INSILICO MODEL / Details: Ab initio model generated in cryoSPARC
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.18 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4) / Number images used: 352937
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial model
ChainPDB IDDetails
chain_id: A, source_name: AlphaFold, initial_model_type: in silico model
chain_id: B, source_name: PDB, initial_model_type: experimental model

6v9i

Initial model consisted of CUL5 from PDB entry 6V9I. Side-chain packing was performed with FASPR.
chain_id: C, source_name: PDB, initial_model_type: experimental model

6v9i

Initial model consisted of RBX2 from PDB entry 6V9I. Side-chain packing was performed with FASPR.
chain_id: D, source_name: PDB, initial_model_type: experimental model

8fvi

Initial model consisted of ELOB from PDB entry 8FVI
chain_id: E, source_name: PDB, initial_model_type: experimental model

8fvi

Initial model consisted of ELOC from PDB entry 8FVI
DetailsAfter initial fitting in ChimeraX, model was energy minimized against the 3D map with Rosetta FastRelax, adjusted in Coot, and refined in Phenix.
RefinementSpace: REAL / Protocol: OTHER

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