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- EMDB-70479: Megrivirus E 3' internal ribosome entry site (IRES) RNA core regi... -

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Basic information

Entry
Database: EMDB / ID: EMD-70479
TitleMegrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome
Map data
Sample
  • Complex: Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome
    • RNA: x 2 types
    • Protein or peptide: x 34 types
  • Ligand: x 1 types
KeywordsRNA / IRES / complex
Function / homology
Function and homology information


laminin receptor activity / ubiquitin ligase inhibitor activity / positive regulation of signal transduction by p53 class mediator / 90S preribosome / phagocytic cup / laminin binding / rough endoplasmic reticulum / translation regulator activity / ribosomal small subunit export from nucleus / gastrulation ...laminin receptor activity / ubiquitin ligase inhibitor activity / positive regulation of signal transduction by p53 class mediator / 90S preribosome / phagocytic cup / laminin binding / rough endoplasmic reticulum / translation regulator activity / ribosomal small subunit export from nucleus / gastrulation / MDM2/MDM4 family protein binding / cytosolic ribosome / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / spindle / rRNA processing / positive regulation of canonical Wnt signaling pathway / rhythmic process / regulation of translation / ribosome binding / virus receptor activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / perikaryon / cytoplasmic translation / cell differentiation / mitochondrial inner membrane / postsynaptic density / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / cell division / DNA repair / mRNA binding / apoptotic process / synapse / dendrite / centrosome / nucleolus / perinuclear region of cytoplasm / Golgi apparatus / DNA binding / RNA binding / zinc ion binding / nucleus / membrane / plasma membrane / cytoplasm
Similarity search - Function
40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Ubiquitin-like protein FUBI / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. ...40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Ubiquitin-like protein FUBI / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein S2, eukaryotic / S27a-like superfamily / 40S Ribosomal protein S10 / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S30 / : / Ribosomal protein S30 / Ribosomal protein S7e signature. / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S27a / Ribosomal protein S17e, conserved site / Ribosomal protein S27a / Ribosomal protein S17e signature. / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S3, eukaryotic/archaeal / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein S27e signature. / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / Ribosomal protein S19A/S15e / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein S19e / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S6, eukaryotic / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / 40S ribosomal protein S1/3, eukaryotes / 40S ribosomal protein S11, N-terminal / Ribosomal_S17 N-terminal / Ribosomal protein S7e / Ribosomal protein S7e / : / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / Ribosomal protein S4e, N-terminal / RS4NT (NUC023) domain / Ribosomal protein S4, KOW domain / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal family S4e / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S6e signature. / Ribosomal protein S24e / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S24e / Ribosomal protein S28e conserved site / Ribosomal protein S28e signature. / Ribosomal protein S27 / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal protein S27 / Ribosomal protein S8e / Ribosomal protein S3Ae / Ribosomal S3Ae family / Ribosomal S3Ae family / Ribosomal protein S28e / Ribosomal protein S28e / Ribosomal protein S6e / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S6e / Ribosomal protein S6e / Ribosomal protein S13/S15, N-terminal / Ribosomal protein S15P / Ribosomal S13/S15 N-terminal domain
Similarity search - Domain/homology
Small ribosomal subunit protein eS32 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein RACK1 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein eS1 ...Small ribosomal subunit protein eS32 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein RACK1 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein uS12 / 40S ribosomal protein S24 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS25 / Small ribosomal subunit protein eS26 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein eS28 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein eS4 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein eS17 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein eS10 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS5
Similarity search - Component
Biological speciesOryctolagus cuniculus (rabbit) / Megrivirus E
Methodsingle particle reconstruction / cryo EM / Resolution: 3.28 Å
AuthorsSherlock ME / Kieft JS
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM118070 United States
CitationJournal: To Be Published
Title: Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome
Authors: Sherlock ME / Kieft JS
History
DepositionMay 2, 2025-
Header (metadata) releaseJan 14, 2026-
Map releaseJan 14, 2026-
UpdateJan 14, 2026-
Current statusJan 14, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_70479.png

Downloads & links

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Map

FileDownload / File: emd_70479.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 0.8255 Å
Density
Contour LevelBy AUTHOR: 0.26
Minimum - Maximum-1.3552437 - 2.2490888
Average (Standard dev.)0.004298112 (±0.07231868)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 422.656 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_70479_additional_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_70479_half_map_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_70479_half_map_2.map
Projections & Slices
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Histogram (log scale)

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Sample components

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Entire : Megrivirus E 3' internal ribosome entry site (IRES) RNA core regi...

EntireName: Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome
Components
  • Complex: Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome
    • RNA: 18S ribosomal RNA
    • RNA: Megrivirus E 3' IRES RNA
    • Protein or peptide: uS2 (SA)
    • Protein or peptide: 40S ribosomal protein S3a
    • Protein or peptide: 40S ribosomal protein S2
    • Protein or peptide: Ribosomal protein S3
    • Protein or peptide: 40S ribosomal protein S4
    • Protein or peptide: Ribosomal protein S5
    • Protein or peptide: 40S ribosomal protein S6
    • Protein or peptide: eS7
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: Ribosomal protein S9 (Predicted)
    • Protein or peptide: S10_plectin domain-containing protein
    • Protein or peptide: Ribosomal protein S11
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: Ribosomal protein S13
    • Protein or peptide: Ribosomal protein S14
    • Protein or peptide: uS19
    • Protein or peptide: Ribosomal protein S16
    • Protein or peptide: eS17
    • Protein or peptide: uS13
    • Protein or peptide: eS19
    • Protein or peptide: uS10
    • Protein or peptide: 40S ribosomal protein S21
    • Protein or peptide: Ribosomal protein S15a
    • Protein or peptide: uS12
    • Protein or peptide: eS24
    • Protein or peptide: eS25
    • Protein or peptide: eS26
    • Protein or peptide: 40S ribosomal protein S27
    • Protein or peptide: Ribosomal protein S28
    • Protein or peptide: eS29
    • Protein or peptide: 40S ribosomal protein S30
    • Protein or peptide: eS31
    • Protein or peptide: RACK1
    • Protein or peptide: eL41
  • Ligand: ZINC ION

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Supramolecule #1: Megrivirus E 3' internal ribosome entry site (IRES) RNA core regi...

SupramoleculeName: Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#36
Details: Purified 80S ribosomes from lysate in complex with in vitro transcribed IRES RNA
Source (natural)Organism: Oryctolagus cuniculus (rabbit)

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Macromolecule #1: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 603.100938 KDa
SequenceString: UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GCUCCUCUCC UACUUGGAUA ACUGUGGUAA U UCUAGAGC ...String:
UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GCUCCUCUCC UACUUGGAUA ACUGUGGUAA U UCUAGAGC UAAUACAUGC CGACGGGCGC UGACCCCCUU CGCGGGGGGG AUGCGUGCAU UUAUCAGAUC AAAACCAACC CG GUCAGCC UCCCCCCGGC CCCGGCCGGG GGGGUGGGCG CCGGCGGCUU UGGUGACUCU AGAUAACCUC GGGCCGAUCG CAC GCCCUC CGUGGCGGCG ACGACCCAUU CGAACGUCUG CCCUAUCAAC UUUCGAUGGU AGUCGCCGUG CCUACCAUGG UGAC CACGG GUGACGGGGA AUCAGGGUUC GAUUCCGGAG AGGGAGCCUG AGAAACGGCU ACCACAUCCA AGGAAGGCAG CAGGC GCGC AAAUUACCCA CUCCCGACCC GGGGAGGUAG UGACGAAAAA UAACAAUACA GGACUCUUUC GAGGCCCUGU AAUUGG AAU GAGUCCACUU UAAAUCCUUU AACGAGGAUC CAUUGGAGGG CAAGUCUGGU GCCAGCAGCC GCGGUAAUUC CAGCUCC AA UAGCGUAUAU UAAAGUUGCU GCAGUUAAAA AGCUCGUAGU UGGAUCUUGG GAGCGGGCGG GCGGUCCGCC GCGAGGCG A GCCACCGCCC GUCCCCGCCC CUUGCCUCUC GGCGCCCCCU CGAUGCUCUU AGCUGAGUGU CCCGCGGGGC CCGAAGCGU UUACUUUGAA AAAAUUAGAG UGUUCAAAGC AGGCCCGAGC CGCCUGGAUA CCGCAGCUAG GAAUAAUGGA AUAGGACCGC GGUUCUAUU UUGUUGGUUU UCGGAACUGA GGCCAUGAUU AAGAGGGACG GCCGGGGGCA UUCGUAUUGC GCCGCUAGAG G UGAAAUUC UUGGACCGGC GCAAGACGGA CCAGAGCGAA AGCAUUUGCC AAGAAUGUUU UCAUUAAUCA AGAACGAAAG UC GGAGGUU CGAAGACGAU CAGAUACCGU CGUAGUUCCG ACCAUAAACG AUGCCGACCG GCGAUGCGGC GGCGUUAUUC CCA UGACCC GCCGGGCAGC UUCCGGGAAA CCAAAGUCUU UGGGUUCCGG GGGGAGUAUG GUUGCAAAGC UGAAACUUAA AGGA AUUUG GCGAAGGGCA CCACCAGGAG UGGAGCCUGC GGCUUAAUUU GACUCAACAC GGGAAACCUC ACCCGGCCCG GACAC GGAC AGGAUUGACA GAUUGAUAGC UCUUUCUCGA UUCCGUGGGU GGUGGUGCAU GGCCGUUCUU AGUUGGUGGA GCGAUU UGU CUGGUUAAUU CCGAUAACGA ACGAGACUCU GGCAUGCUAA CUAGUUACGC GACCCCCGAG CGGUCGGCGU CCCCCAA CU UCUUAGAGGG ACAAGUGGCG UUCAGCCACC CGAGAUUGAG CAAUAACAGG UCUGUGAUGC CCUUAGAUGU CCGGGGCU G CACGCGCGCU ACACUGACUG GCUCAGCGUG UGCCUACCCU ACGCCGGCAG GCGCGGGUAA CCCGUUGAAC CCCAUUCGU GAUGGGGAUC GGGGAUUGCA AUUAUUCCCC AUGAACGAGG AAUUCCCAGU AAGUGCGGGU CAUAAGCUUG CGUUGAUUAA GUCCCUGCC CUUUGUACAC ACCGCCCGUC GCUACUACCG AUUGGAUGGU UUAGUGAGGC CCUCGGAUCG GCCCCGCCGG G GUCGGCCC ACGGCCCUGG CGGAGCGCUG AGAAGACGGU CGAACUUGAC UAUCUAGAGG AAGUAAAAGU CGUAACAAGG UU UCCGUAG GUGAACCUGC GGAAGGAUCA UUA

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Macromolecule #2: Megrivirus E 3' IRES RNA

MacromoleculeName: Megrivirus E 3' IRES RNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Megrivirus E
Molecular weightTheoretical: 86.668852 KDa
SequenceString: CUUCAGGGUC UUAGUGUUGU GGGAUACCCU GUCGUACACG UAAAACACCC ACCUCCUAGC UCAUGGAGUU AAAUUGAGUA GCCGUUGGA AUCGGCUAUA UAAAUUAUCC AGGAACCUGU CGAGUAGUUG ACCUUAUGCU UUCACAGACG UGUUUUCUUC C UGAAGCAU ...String:
CUUCAGGGUC UUAGUGUUGU GGGAUACCCU GUCGUACACG UAAAACACCC ACCUCCUAGC UCAUGGAGUU AAAUUGAGUA GCCGUUGGA AUCGGCUAUA UAAAUUAUCC AGGAACCUGU CGAGUAGUUG ACCUUAUGCU UUCACAGACG UGUUUUCUUC C UGAAGCAU UUGGAAUAGU GGGCUUGGGA AAACCCACCU UGGACUAUAC GGCCUGAUAG GGUGCUGUUG CUACCAGCAC AG GUUUCUU GAUCCGUAGC GGGUUGUAAU AUG

GENBANK: GENBANK: MN453780.1

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Macromolecule #3: uS2 (SA)

MacromoleculeName: uS2 (SA) / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 32.958016 KDa
SequenceString: MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQTAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA ...String:
MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQTAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA HSVGLMWWML AREVLRMRGT ISREHPWEVM PDLYFYRDPE EIEKEEQAAA EKAVTKEEFQ GEWTAPAPEF TA TQPEVAD WSEGVQVPSV PIQQFPTEDW SAQPATEDWS AAPTAQATEW VGTTTEWS

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #4: 40S ribosomal protein S3a

MacromoleculeName: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 30.002061 KDa
SequenceString: MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM ...String:
MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGSSS GK ATGDETG AKVERADGYE PPVQESV

UniProtKB: Small ribosomal subunit protein eS1

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Macromolecule #5: 40S ribosomal protein S2

MacromoleculeName: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 24.441846 KDa
SequenceString: KEWLPVTKLG RLVKDMKIKS LEEIYLFSLP IKESEIIDFF LGASLKDEVL KIMPVQKQTR AGQRTRFKAF VAIGDYNGHV GLGVKCSKE VATAIRGAII LAKLSIVPVR RGYWGNKIGK PHTVPCKVTG RCGSVLVRLI PAPRGTGIVS APVPKKLLMM A GIDDCYTS ...String:
KEWLPVTKLG RLVKDMKIKS LEEIYLFSLP IKESEIIDFF LGASLKDEVL KIMPVQKQTR AGQRTRFKAF VAIGDYNGHV GLGVKCSKE VATAIRGAII LAKLSIVPVR RGYWGNKIGK PHTVPCKVTG RCGSVLVRLI PAPRGTGIVS APVPKKLLMM A GIDDCYTS ARGCTATLGN FAKATFDAIS KTYSYLTPDL WKETVFTKSP YQEFTDHLVK THT

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #6: Ribosomal protein S3

MacromoleculeName: Ribosomal protein S3 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 31.146607 KDa
SequenceString: MSARRRRRRA AFRRRAEPFI PISVREPLPF LSAARGGKMA VQISKKRKFV ADGIFKAELN EFLTRELAED GYSGVEVRVT PTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG V LRFIMESG ...String:
MSARRRRRRA AFRRRAEPFI PISVREPLPF LSAARGGKMA VQISKKRKFV ADGIFKAELN EFLTRELAED GYSGVEVRVT PTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG V LRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPSGKIGP KK PLPDHVS IVEPKDEILP TTPISEQKGG KPEPPAMPQP VPTA

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #7: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 29.654869 KDa
SequenceString: MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ...String:
MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

UniProtKB: Small ribosomal subunit protein eS4

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Macromolecule #8: Ribosomal protein S5

MacromoleculeName: Ribosomal protein S5 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 22.913453 KDa
SequenceString: MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT ...String:
MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #9: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 28.751906 KDa
SequenceString: MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK ...String:
MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSLR AS TSKSESS QK

UniProtKB: Small ribosomal subunit protein eS6

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Macromolecule #10: eS7

MacromoleculeName: eS7 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 47.356172 KDa
SequenceString: EPITNRVFQA LTSSDFKQQD GGSFSLSRTH LSGFRSRPRR TTIASAATRP PPIPSRGGSA PTGTPEGAGA SRSSRTVPRL PPQPSGPPR APRHPGFQRA LPSLIRPEPG VRAPLLASAG TRFKRPASQA VKALSARGLL QPQVQVGPRA NRVPQRREPR Q HLSGHAPG ...String:
EPITNRVFQA LTSSDFKQQD GGSFSLSRTH LSGFRSRPRR TTIASAATRP PPIPSRGGSA PTGTPEGAGA SRSSRTVPRL PPQPSGPPR APRHPGFQRA LPSLIRPEPG VRAPLLASAG TRFKRPASQA VKALSARGLL QPQVQVGPRA NRVPQRREPR Q HLSGHAPG ASEGGSASPT YWSLGITSWD ALGFPLRLGG SSGFRSSVAL FRPLSFRRGD LPRSREIWAS SSAGAQPGEA MF SSSAKIV KPNGEKPDEF ESGISQALLE LEMNSDLKAQ LRELNITAAK EIEVGGGRKA IIIFVPVPQL KSFQKIQVRL VRE LEKKFS GKHVVFIAQR RILPKPTRKS RTKNKQKRPR SRTLTAVHDA ILEDLVFPSE IVGKRIRVKL DGSRLIKVHL DKAQ QNNVE HKVETFSGVY KKLTGKDVNF EFPEFQL

UniProtKB: Small ribosomal subunit protein eS7

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Macromolecule #11: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 24.263387 KDa
SequenceString: MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ ...String:
MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ GKLLACIASR PGQCGRADGY VLEGKELEFY LRKIKARKGK

UniProtKB: Small ribosomal subunit protein eS8

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Macromolecule #12: Ribosomal protein S9 (Predicted)

MacromoleculeName: Ribosomal protein S9 (Predicted) / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 22.641564 KDa
SequenceString: MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG ...String:
MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG DDEEED

UniProtKB: Small ribosomal subunit protein uS4

+
Macromolecule #13: S10_plectin domain-containing protein

MacromoleculeName: S10_plectin domain-containing protein / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.156865 KDa
SequenceString:
MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSRP ETGRPRPKGL EGERPARLTR GEADRDTYRR SAVPPGADKK AEAGAGSATE

UniProtKB: Small ribosomal subunit protein eS10

+
Macromolecule #14: Ribosomal protein S11

MacromoleculeName: Ribosomal protein S11 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 18.468826 KDa
SequenceString:
MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

UniProtKB: Small ribosomal subunit protein uS17

+
Macromolecule #15: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.538987 KDa
SequenceString:
MAEEGIAAGG VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VLASNCDEPM YVKLVEALCA EHQINLIKVD DNKKLGEWV GLCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK CKK

UniProtKB: Small ribosomal subunit protein eS12

+
Macromolecule #16: Ribosomal protein S13

MacromoleculeName: Ribosomal protein S13 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.259389 KDa
SequenceString:
MGRMHAPGKG LSQSALPYRR SVPTWLKLTS DDVKEQIYKL AKKGLTPSQI GVILRDSHGV AQVRFVTGNK ILRILKSKGL APDLPEDLY HLIKKAVAVR KHLERNRKDK DAKFRLILIE SRIHRLARYY KTKRVLPPNW KYESSTASAL VA

UniProtKB: Small ribosomal subunit protein uS15

+
Macromolecule #17: Ribosomal protein S14

MacromoleculeName: Ribosomal protein S14 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 18.133984 KDa
SequenceString:
MKARALSGSG VRRRRAAMAP RKGKEKKEEQ VISLGPQVAE GENVFGVCHI FASFNDTFVH VTDLSGKETI CRVTGGMKVK ADRDESSPY AAMLAAQDVA QRCKELGITA LHIKLRATGG NRTKTPGPGA QSALRALARS GMKIGRIEDV TPIPSDSTRR K GGRRGRRL

UniProtKB: Small ribosomal subunit protein uS11

+
Macromolecule #18: uS19

MacromoleculeName: uS19 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.049182 KDa
SequenceString:
MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLSRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

UniProtKB: Small ribosomal subunit protein uS19

+
Macromolecule #19: Ribosomal protein S16

MacromoleculeName: Ribosomal protein S16 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 19.213465 KDa
SequenceString:
SARVAPRFPF PVVAPRGEDR HSSAAAMPSK GPLQSVQVFG RKKTATAVAH CKRGNGLIKV NGRPLEMIEP RTLQYKLLEP VLLLGKERF AGVDIRVRVK GGGHVAQIYA IRQSISKALV AYYQKYVDEA SKKEIKDILI QYDRTLLVAD PRRCESKKFG G PGARARYQ KSYR

UniProtKB: Small ribosomal subunit protein uS9

+
Macromolecule #20: eS17

MacromoleculeName: eS17 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 15.552119 KDa
SequenceString:
MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGAV

UniProtKB: Small ribosomal subunit protein eS17

+
Macromolecule #21: uS13

MacromoleculeName: uS13 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.759777 KDa
SequenceString:
MSLVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQYKIP DWFLNRQKD VKDGKYSQVL ANGLDNKLRE DLERLKKIRA HRGLRHFWGL RVRGQHTKTT GRRGRTVGVS KKK

UniProtKB: Small ribosomal subunit protein uS13

+
Macromolecule #22: eS19

MacromoleculeName: eS19 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 16.235796 KDa
SequenceString:
MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYLRGGA GVGSMTKIYG GRQRNGVMP SHFSRGSKSV ARRVLQALEG LKMVEKDQDW GRKLTPQGQR DLDRIAGQVA AAKKKH

UniProtKB: Small ribosomal subunit protein eS19

+
Macromolecule #23: uS10

MacromoleculeName: uS10 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 13.398763 KDa
SequenceString:
MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT LRITTRKTPC GEGSKTWDRF QMRIHKRLI DLHSPSEIVK QITSISIEPG VEVEVTIADA

UniProtKB: Small ribosomal subunit protein uS10

+
Macromolecule #24: 40S ribosomal protein S21

MacromoleculeName: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 9.124389 KDa
SequenceString:
MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS KNF

UniProtKB: Small ribosomal subunit protein eS21

+
Macromolecule #25: Ribosomal protein S15a

MacromoleculeName: Ribosomal protein S15a / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.865555 KDa
SequenceString:
MVRMNVLADA LKSINNAEKR GKRQVLIRPC SKVIVRFLTV MMKHGYIGEF EIIDDHRAGK IVVNLTGRLN KCGVISPRFD VQLKDLEKW QNNLLPSRQF GFIVLTTSAG IMDHEEARRK HTGGKILGFF F

UniProtKB: Small ribosomal subunit protein uS8

+
Macromolecule #26: uS12

MacromoleculeName: uS12 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 15.844666 KDa
SequenceString:
MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK QPNSAIRKCV RVQLIKNGKK ITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPRS

UniProtKB: Small ribosomal subunit protein uS12

+
Macromolecule #27: eS24

MacromoleculeName: eS24 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 15.237104 KDa
SequenceString:
MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI REKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK KK

UniProtKB: 40S ribosomal protein S24

+
Macromolecule #28: eS25

MacromoleculeName: eS25 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 13.645028 KDa
SequenceString:
PPKDDKKKKD AGKSAKKDKD PVNKSGGKAK KKKWSKGKVR DKLNNLVLFD KATYDKLCKE VPNYKLITPA VVSERLKIRG SLARAALQE LLSKGLIKLV SKHRAQVIYT RNTKGGDAPA AGEDA

UniProtKB: Small ribosomal subunit protein eS25

+
Macromolecule #29: eS26

MacromoleculeName: eS26 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 11.645794 KDa
SequenceString:
TKKRRNNGRA KKGRGHVQPI RCTNCARCVP KDKAIKKFVI RNIVEAAAVR DISEASVFDA YVLPKLYVKL HYCVSCAIHS KVVRNRSRE ARKDRTPPPR FR

UniProtKB: Small ribosomal subunit protein eS26

+
Macromolecule #30: 40S ribosomal protein S27

MacromoleculeName: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 9.480186 KDa
SequenceString:
MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

UniProtKB: Small ribosomal subunit protein eS27

+
Macromolecule #31: Ribosomal protein S28

MacromoleculeName: Ribosomal protein S28 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 7.855052 KDa
SequenceString:
MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

UniProtKB: Small ribosomal subunit protein eS28

+
Macromolecule #32: eS29

MacromoleculeName: eS29 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 6.690821 KDa
SequenceString:
MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

UniProtKB: Small ribosomal subunit protein uS14

+
Macromolecule #33: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.498884 KDa
SequenceString:
MQLFVRAQEL HTLEVTGRET VAQIKAHVAS LEGIAPEDQV VLLAGTPLED EATLGQCGVE ALSTLEVAGR MLGGKVHGSL ARVGKVRGQ TLKVAKQEKK KKRTGRAKRR MQYNRRFVNV VPTFGKKKGP NANS

UniProtKB: Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein

+
Macromolecule #34: eS31

MacromoleculeName: eS31 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 21.431742 KDa
SequenceString:
MARHPLYQGS GIWCGRLRGG AFGGGAGKPR GPAALFLFDP PSARRWARHQ DADLRENPYG EDHHARGIPP DQQRLIFAGK QLEDGRTLS DYNIQKESTL HLVLRLRGGA KKRKKKSYTT PKKNKHKRKK VKLAVLKYYK VDENGKISRL RRECPSDECG A GVFMASHF DRHYCGKCCL TYCFNKPEDK

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

+
Macromolecule #35: RACK1

MacromoleculeName: RACK1 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 35.115652 KDa
SequenceString: MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC ...String:
MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC GWDKLVKVWN LANCKLKTNH IGHTGYLNTV TVSPDGSLCA SGGKDGQAML WDLNEGKHLY TLDGGDIINA LC FSPNRYW LCAATGPSIK IWDLEGKIIV DELKQEVIST SSKAEPPQCT SLAWSADGQT LFAGYTDNLV RVWQVTIGTR

UniProtKB: Small ribosomal subunit protein RACK1

+
Macromolecule #36: eL41

MacromoleculeName: eL41 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 3.473451 KDa
SequenceString:
MRAKWRKKRM RRLKRKRRKM RQRSK

UniProtKB: Small ribosomal subunit protein eS32

+
Macromolecule #37: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 37 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5 / Details: 20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
GridModel: C-flat-1.2/1.3 / Material: COPPER / Mesh: 400 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 6 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV
Details80S ribosomes at 200nM IRES RNA at 1uM

-
Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOCONTINUUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.4 µm / Nominal magnification: 105000
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

CTF correctionSoftware - Name: cryoSPARC / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

7syp


Details: 7SYP used for 40S model, 5A2Q used from initial IRES model
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.28 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 64736
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: MAXIMUM LIKELIHOOD

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