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- EMDB-70375: Structure of the MOR/Gi/DAMGO Complex, GTP-Bound, G-Primed, AHD-Sorted -

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Open data


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Basic information

Entry
Database: EMDB / ID: EMD-70375
TitleStructure of the MOR/Gi/DAMGO Complex, GTP-Bound, G-Primed, AHD-Sorted
Map data
Sample
  • Complex: Mu Opioid Receptor Gi1 heterotrimer complex
KeywordsGPCR / Complex / Agonist / MEMBRANE PROTEIN
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.7 Å
AuthorsRobertson MJ / Skiniotis G
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health & Human Development (NIH/NICHD)HD107581 United States
Cancer Prevention and Research Institute of Texas (CPRIT)RR230042 United States
CitationJournal: To Be Published
Title: Non-equilibrium Structural Pharmacology of the mu-Opioid Receptor
Authors: Robertson MJ / Papasergi-Scott MM / Peroto MC / Varga B / Majumdar S / Skiniotis G
History
DepositionApr 27, 2025-
Header (metadata) releaseMar 4, 2026-
Map releaseMar 4, 2026-
UpdateMar 4, 2026-
Current statusMar 4, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_70375.png

Downloads & links

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Map

FileDownload / File: emd_70375.map.gz / Format: CCP4 / Size: 209.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.87 Å/pix.
x 380 pix.
= 329.726 Å		0.87 Å/pix.
x 380 pix.
= 329.726 Å		0.87 Å/pix.
x 380 pix.
= 329.726 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.8677 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.36
Minimum - Maximum-1.8458287 - 2.9815912
Average (Standard dev.)-0.00035065247 (±0.037590533)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions380380380
Spacing380380380
CellA=B=C: 329.72598 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_70375_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_70375_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Mu Opioid Receptor Gi1 heterotrimer complex

EntireName: Mu Opioid Receptor Gi1 heterotrimer complex
Components
  • Complex: Mu Opioid Receptor Gi1 heterotrimer complex

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Supramolecule #1: Mu Opioid Receptor Gi1 heterotrimer complex

SupramoleculeName: Mu Opioid Receptor Gi1 heterotrimer complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #4, #1-#3
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.6 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 854816
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION

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