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- EMDB-70141: The dimeric KICSTOR-GATOR1 supercomplex -

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Basic information

Entry
Database: EMDB / ID: EMD-70141
TitleThe dimeric KICSTOR-GATOR1 supercomplex
Map dataComposite reconstruction
Sample
  • Complex: Dimeric supercomplex of KICSTOR-GATOR1
    • Complex: dimeric GATOR1 subcomplex
      • Protein or peptide: GATOR complex protein NPRL3
      • Protein or peptide: GATOR complex protein NPRL2
      • Protein or peptide: GATOR1 complex protein DEPDC5
    • Complex: SZT2 component of the KICSTOR subcomplex
      • Protein or peptide: KICSTOR complex protein SZT2
KeywordsLysosome / GATOR1 / KICSTOR / cell growth / amino acid sensing / mTOR / KPTN / ITFG2 / C12orf66 / SZT2 / NPRL2 / NPRL3 / DEPDC5 / CELL CYCLE
Function / homology
Function and homology information


regulation of superoxide dismutase activity / KICSTOR complex / GATOR1 complex / negative regulation of kinase activity / corpus callosum morphogenesis / aorta morphogenesis / protein localization to lysosome / Amino acids regulate mTORC1 / cardiac muscle tissue development / vacuolar membrane ...regulation of superoxide dismutase activity / KICSTOR complex / GATOR1 complex / negative regulation of kinase activity / corpus callosum morphogenesis / aorta morphogenesis / protein localization to lysosome / Amino acids regulate mTORC1 / cardiac muscle tissue development / vacuolar membrane / ventricular septum development / pigmentation / roof of mouth development / cellular response to glucose starvation / negative regulation of TORC1 signaling / positive regulation of autophagy / GTPase activator activity / cellular response to amino acid starvation / central nervous system development / post-embryonic development / small GTPase binding / peroxisome / lysosome / intracellular signal transduction / lysosomal membrane / protein-containing complex binding / perinuclear region of cytoplasm / cytosol
Similarity search - Function
Protein SZT2 / : / IML1 N-terminal double psi beta barrel domain / Nitrogen permease regulator 3 / Nitrogen permease regulator 2 / Vacuolar membrane-associated protein Iml1 / DEPDC5 protein, C-terminal / : / : / Nitrogen Permease regulator of amino acid transport activity 3 ...Protein SZT2 / : / IML1 N-terminal double psi beta barrel domain / Nitrogen permease regulator 3 / Nitrogen permease regulator 2 / Vacuolar membrane-associated protein Iml1 / DEPDC5 protein, C-terminal / : / : / Nitrogen Permease regulator of amino acid transport activity 3 / Nitrogen permease regulator 2 / Vacuolar membrane-associated protein Iml1, N-terminal domain / DEPDC5 protein C-terminal region / GATOR1 complex protein NPRL3, C-terminal HTH / Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP) / DEP domain profile. / Domain found in Dishevelled, Egl-10, and Pleckstrin / DEP domain / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily
Similarity search - Domain/homology
GATOR1 complex protein DEPDC5 / GATOR1 complex protein NPRL3 / KICSTOR complex protein SZT2 / GATOR1 complex protein NPRL2
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.0 Å
AuthorsBayly-Jones C / Lupton CJ / Chang YG / Ellisdon AM
Funding support Australia, 4 items
OrganizationGrant numberCountry
National Health and Medical Research Council (NHMRC, Australia)APP2036864 Australia
Australian Research Council (ARC)DE240100992 Australia
Other governmentMCRF21036 Australia
Australian Research Council (ARC)LE170100016 Australia
CitationJournal: Cell / Year: 2026
Title: Structure of the lysosomal KICSTOR-GATOR1-SAMTOR nutrient-sensing supercomplex.
Authors: Christopher J Lupton / Charles Bayly-Jones / Shuqi Dong / Terrance Lam / Wentong Luo / Gareth D Jones / Chantel Mastos / Nicholas J Frescher / San S Lim / Alastair C Keen / Luke E Formosa / ...Authors: Christopher J Lupton / Charles Bayly-Jones / Shuqi Dong / Terrance Lam / Wentong Luo / Gareth D Jones / Chantel Mastos / Nicholas J Frescher / San S Lim / Alastair C Keen / Luke E Formosa / Hari Venugopal / Yong-Gang Chang / Michelle L Halls / Andrew M Ellisdon /
Abstract: The guanosine triphosphate (GTP)-bound state of the heterodimeric Rag GTPases functions as a molecular switch regulating mechanistic target of rapamycin complex 1 (mTORC1) activation at the lysosome ...The guanosine triphosphate (GTP)-bound state of the heterodimeric Rag GTPases functions as a molecular switch regulating mechanistic target of rapamycin complex 1 (mTORC1) activation at the lysosome downstream of amino acid fluctuations. Under low amino acid conditions, GTPase-activating protein (GAP) activity toward Rags 1 (GATOR1) promotes RagA GTP hydrolysis, preventing mTORC1 activation. KICSTOR recruits and regulates GATOR1 at the lysosome by undefined mechanisms. Here, we resolve the KICSTOR-GATOR1 structure, revealing a striking ∼60-nm crescent-shaped assembly. GATOR1 anchors to KICSTOR via an extensive interface, and mutations that disrupt this interaction impair mTORC1 regulation. The S-adenosylmethionine sensor SAMTOR binds KICSTOR in a manner incompatible with metabolite binding, providing structural insight into methionine sensing via SAMTOR-KICSTOR association. We discover that KICSTOR and GATOR1 form a dimeric supercomplex. This assembly restricts GATOR1 to an orientation that favors the low-affinity active GAP mode of Rag GTPase engagement while sterically restricting access to the high-affinity inhibitory mode, consistent with a model of an active lysosomal GATOR1 docking complex.
History
DepositionApr 10, 2025-
Header (metadata) releaseJan 21, 2026-
Map releaseJan 21, 2026-
UpdateJan 21, 2026-
Current statusJan 21, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_70141.png

Downloads & links

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Map

FileDownload / File: emd_70141.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationComposite reconstruction
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1 Å/pix.
x 320 pix.
= 320. Å		1 Å/pix.
x 320 pix.
= 320. Å		1 Å/pix.
x 320 pix.
= 320. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 3.0
Minimum - Maximum-0.06421983 - 11.511986
Average (Standard dev.)0.08265377 (±0.6125606)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 320.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Dimeric supercomplex of KICSTOR-GATOR1

EntireName: Dimeric supercomplex of KICSTOR-GATOR1
Components
  • Complex: Dimeric supercomplex of KICSTOR-GATOR1
    • Complex: dimeric GATOR1 subcomplex
      • Protein or peptide: GATOR complex protein NPRL3
      • Protein or peptide: GATOR complex protein NPRL2
      • Protein or peptide: GATOR1 complex protein DEPDC5
    • Complex: SZT2 component of the KICSTOR subcomplex
      • Protein or peptide: KICSTOR complex protein SZT2

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Supramolecule #1: Dimeric supercomplex of KICSTOR-GATOR1

SupramoleculeName: Dimeric supercomplex of KICSTOR-GATOR1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 582 KDa

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Supramolecule #2: dimeric GATOR1 subcomplex

SupramoleculeName: dimeric GATOR1 subcomplex / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#2, #4
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #3: SZT2 component of the KICSTOR subcomplex

SupramoleculeName: SZT2 component of the KICSTOR subcomplex / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #3
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: GATOR complex protein NPRL3

MacromoleculeName: GATOR complex protein NPRL3 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 65.769 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MGYPYDVPDY ADLNGGGGGS TMRDNTSPIS VILVSSGSRG NKLLFRYPFQ RSQEHPASQT SKPRSRYAAS NTGDHADEQD GDSRFSDVI LATILATKSE MCGQKFELKI DNVRFVGHPT LLQHALGQIS KTDPSPKREA PTMILFNVVF ALRANADPSV I NCLHNLSR ...String:
MGYPYDVPDY ADLNGGGGGS TMRDNTSPIS VILVSSGSRG NKLLFRYPFQ RSQEHPASQT SKPRSRYAAS NTGDHADEQD GDSRFSDVI LATILATKSE MCGQKFELKI DNVRFVGHPT LLQHALGQIS KTDPSPKREA PTMILFNVVF ALRANADPSV I NCLHNLSR RIATVLQHEE RRCQYLTREA KLILALQDEV SAMADGNEGP QSPFHHILPK CKLARDLKEA YDSLCTSGVV RL HINSWLE VSFCLPHKIH YAASSLIPPE AIERSLKAIR PYHALLLLSD EKSLLGELPI DCSPALVRVI KTTSAVKNLQ QLA QDADLA LLQVFQLAAH LVYWGKAIII YPLCENNVYM LSPNASVCLY SPLAEQFSHQ FPSHDLPSVL AKFSLPVSLS EFRN PLAPA VQETQLIQMV VWMLQRRLLI QLHTYVCLMA SPSEEEPRPR EDDVPFTARV GGRSLSTPNA LSFGSPTSSD DMTLT SPSM DNSSAELLPS GDSPLNQRMT ENLLASLSEH ERAAILSVPA AQNPEDLRMF ARLLHYFRGR HHLEEIMYNE NTRRSQ LLM LFDKFRSVLV VTTHEDPVIA VFQALLP

UniProtKB: GATOR1 complex protein NPRL3

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Macromolecule #2: GATOR complex protein NPRL2

MacromoleculeName: GATOR complex protein NPRL2 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 45.799547 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MGYPYDVPDY ADLNGGGGGS TMGSGCRIEC IFFSEFHPTL GPKITYQVPE DFISRELFDT VQVYIITKPE LQNKLITVTA MEKKLIGCP VCIEHKKYSR NALLFNLGFV CDAQAKTCAL EPIVKKLAGY LTTLELESSF VSMEESKQKL VPIMTILLEE L NASGRCTL ...String:
MGYPYDVPDY ADLNGGGGGS TMGSGCRIEC IFFSEFHPTL GPKITYQVPE DFISRELFDT VQVYIITKPE LQNKLITVTA MEKKLIGCP VCIEHKKYSR NALLFNLGFV CDAQAKTCAL EPIVKKLAGY LTTLELESSF VSMEESKQKL VPIMTILLEE L NASGRCTL PIDESNTIHL KVIEQRPDPP VAQEYDVPVF TKDKEDFFNS QWDLTTQQIL PYIDGFRHIQ KISAEADVEL NL VRIAIQN LLYYGVVTLV SILQYSNVYC PTPKVQDLVD DKSLQEACLS YVTKQGHKRA SLRDVFQLYC SLSPGTTVRD LIG RHPQQL QHVDERKLIQ FGLMKNLIRR LQKYPVRVTR EEQSHPARLY TGCHSYDEIC CKTGMSYHEL DERLENDPNI IICW K

UniProtKB: GATOR1 complex protein NPRL2

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Macromolecule #3: KICSTOR complex protein SZT2

MacromoleculeName: KICSTOR complex protein SZT2 / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 380.3865 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MEMDYKDDDD KGGGGGASTM ASERPEPEVE EAGQVFLLMK KDYRISRNVR LAWFLSHLHQ TVQATPQEML LQSEQELEVL SVLPPGWQP DEPVVPRPFL LVPSTRVTFL AWQYRFVIEL DLSPSTGIVD DSTGEILFDE VFHALSRCLG GLLRPFRVPG S CIDFQPEI ...String:
MEMDYKDDDD KGGGGGASTM ASERPEPEVE EAGQVFLLMK KDYRISRNVR LAWFLSHLHQ TVQATPQEML LQSEQELEVL SVLPPGWQP DEPVVPRPFL LVPSTRVTFL AWQYRFVIEL DLSPSTGIVD DSTGEILFDE VFHALSRCLG GLLRPFRVPG S CIDFQPEI YVTIQAYSSI IGLQSHQVLV QGCLLDPSQR EVFLQQIYEQ LCLFEDKVAT MLQQQYDPQS QAEDQSPDSG DL LGRKVGV SMVTADLGLV SMIRQGILAL QLLPSNSSAG IIVITDGVTS VPDVAVCETL LNQLRSGTVA CSFVQVGGVY SYD CSFGHV PNVELMKFIA MATFGSYLST CPEPEPGNLG LTVYHRAFLL YSFLRSGEAL NPEYYCGSQH RLFNEHLVSA SSNP ALALR RKKHTEKEVP ADLVSTVSVR LREGYSVREV TLAKGGSQLE VKLVLLWKHN MRIEYVAMAP WPLEPEGPRV TRVEV TMEG GYDILHDVSC ALRQPIRSLY RTHVIRRFWN TLQSINQTDQ MLAHLQSFSS VPEHFTLPDS TKSGVPLFYI PPGSTT PVL SLQPSGSDSS HAQFAAYWKP VLSMDANSWQ RWLHMHRLVL ILEHDTPIPK HLHTPGSNGR YSTIQCRISH SSLTSLL RD WSSFVLVEGY SYVKLLSSAP DQPPNSFYMV RIISKAPCMV LRLGFPIGTP APARHKIVSG LREEILRLRF PHRVQSKE P TPKVKRKGLG GAGGGSSPSK SPPVLGPQQA LSDRPCLVVL HKPLDKLLIR YEKLPLDYRA PFLLTLEPPG PLPLVSGRS ASSSLASLSR YLYHQRWLWS VPSGLAPALP LSAIAQLLSI LTEVRLSEGF HFACSGEGII NMVLELPIQN EPPGQAAAEE KHTCVVQYI LFPPHSTSTK DSFSTDDDND VEVEALEGDS ELNLVTEVWV EPQYGRVGPG PGIWKHLQDL TYSEIPQALH P RDAACIGS MLSFEYLIQL CQSKEWGPLP PEPRVSDGLD QGGDTCVHEI PFHFDLMGLL PQCQQLQMFF LLLAREPEGV PF AEGSCPA NDMVLCLLHS CLGQELSDRE IPLTPVDQAA FLSEVLRRTC HVPGAEGPLL GVHGIPKEQA VGSTQATGDS AFT SLSVGL PETLKPLISA QPPQWRCYAR LVNPQHVFLT FLPATFSDVQ RLAACGLEGP PQEETKPKFG DWSGAPSLKD LGGT GIKAT KSHVPVLSVT LASDNAQNQG ELSPPFRRDL QAYAGRQASQ TESADGPRTR CPVYIYSCSL EALREQMVGM QPPQA PRDL IFRTQFLDHP SPSSAWMEPR YKEAANHCAL LQEHAQRCYV RGLFRSLQQA QSVTSQDLLT AVDACEELLQ EIDITP FLL ALCGHTWGLP HAPPSPGPLS PGPFSSSMEE GAEPRERAIL ASESSIETED LSEPEFQSTR VPGIPDPGPE ISLTDVC QL RGEAHGALHS VIQEKFLEIS RLHFRTVPSN PHYFFYCPPS SRREDEGPRD TVDRKISDLE FSEAELMGEE GDTSACCV V TESDPELEVE YRESRESDLG PAGLDSASLS DVDTVNPDED SFSILGGDSP TGPESFLHDL PPLFLHLTCS VRLRGQHSS VPVCSLPTCL GQVLSSLEGP PVGGRVPLRD LSVTLDVFML TLPLEVELPT ASDPQHHRST SESSASFPRS PGQPSSLRSD DGLGPPLPP PEEERHPGLS NLATPHRLAI ETTMNEIRWL LEDEMVGALR RGGIPQSPAL HRAAAHIHSS PGRSTCLRQT L PLSFVFGP ERSLTQFKEE FRRLHLPGHV LLEDPDSGFF FVAAGQQPGG SHGEPSSAAW AWHSHEDRAE GIEGETLTAS PQ APGSPED SEGVPLISLP RVPQGGSQPG PSRGLSLMSS QGSVDSDHLG YDGGSSGSDS EGPNDTLGEK APFTLRTPPG PAP PQPSLS GLPGPCLPDF WLIVRVLQDR VEVYAHARSL IREDGGPGTE CRHLQQLLVR RVGEICREVN QRLLLQDLHD SHVC NSLLV AESEEDLWRS ETPFHSRQRA PLPSDDYAAD ESCAPRGYLA ATMQFVPGHF SCDVVWGTVI RVHSRLKMGP SMGVS RAIQ ALRSVLNAFS VVNRKNMFVY QERATKAVYY LRLLETSCSD RPWKGDALPP SLALSRSQEP IYSEEASGPR SPLDMV SSR SSDAARPVGQ VDRHIQLLVH GVGQAGPEIT DELVRVLCRR LDEATLDVIT VMLVRNCKLT PADVEFIQPP GSLPSEV LH LALPTSCRPW LPALAWYLRQ NLLIFLHSPK YTDSNSRNHF QHPLPPQGGL PDLDIYLYNK PGGQGTGGKG VACITLAF V DEGGAPLSLA LWPPSSPGPP DPLREEEFEQ LTQVIRCPVV VDSSSAQNGA PRLRLDVWEK GNISIVQLEE KLRGAARQA LADAIIELQL LPASLCTEDT PTGSLRNGSL ETKSSAGRAS TFPPAPVPGE PVTPPSKAGR RSFWDMLSKT ECGDLGSPKT TDDIVLDRP EDTRGRRRHK TESVRTPGGA ERAPGSDSGA QRQKRRTTQL EEGEVGTLHP VFARVAQRWM EFMVQIGCAS V SRSSAHMV SRFLLPSILS EFTALVTSMA GDTSVRIFEQ HLGSEPEIFG PCSPGQLGPS PRPAAERHLL LLGRNFLQWR RP TQQAAKA MQRFEPGGDG SSGRNAPRQR LLLLEVVDKK LQLLTYNWAP DLGAALGRAL VRLVQWQNAR AHLIFCLLSQ KLG LFHHYG QLDFPVRDEK EPNPFLLPTM EVETLIRSAS PPLSREQGRL SGSSRGGGPL PLDTFPFDEA LRDITAARPS SVLG PVPRP PDPVTYHGQQ FLEIKMAERR ELERQMKMEN LFVTWQQRST PATMPISAGE LETLKQSSRL VHYCATAMLF DPAAW LHGP PETSGPPDGQ RRHRPESGSG SREAPTSCES LDVSPPGARE EPWLKELSLA FLQQYVQYLQ SIGFVLVPLR PPSPAR STS RPRAMAILGT EGRGSFSCPK TKTDGSPKST SSPVTTYHLQ RALPGGIILM ELAFQGCYFC VKQFALECSR IPMGQAV NS QLSMLFTEEC DKVRDLMHVH SFSYDFHLRL VHQHVLGAHL VLRHGYHLTT FLRHFLAHHP DGPHFGRNHI YQGTLELP T PLIAAHQLYN YVADHASSYH MKPLRMARPG GPEHNEYALV SAWHSSGSYL DSEGLRHQDD FDVSLLVCHC AAPFEEQGE AERHVLRLQF FVVLTSQREL FPRLTADMRR FRKPPRLPPE PEAPGSSAGS PGEASGLILA PGPAPLFPPL AAEVGMARAR LAQLVRLAG GHCRRDTLWK RLFLLEPPGP DRLRLGGRLA LAELEELLEA VHAKSIGDID PQLDCFLSMT VSWYQSLIKV L LSRFPQSC RHFQSPDLGT QYLVVLNQKF TDCFVLVFLD SHLGKTSLTV VFREPFPVQP QDSESPPAQL VSTYHHLESV IN TACFTLW TRLL

UniProtKB: KICSTOR complex protein SZT2

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Macromolecule #4: GATOR1 complex protein DEPDC5

MacromoleculeName: GATOR1 complex protein DEPDC5 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 183.207703 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MGDYKDDDDK GGGGGASTMR TTKVYKLVIH KKGFGGSDDE LVVNPKVFPH IKLGDIVEIA HPNDEYSPLL LQVKSLKEDL QKETISVDQ TVTQVFRLRP YQDVYVNVVD PKDVTLDLVE LTFKDQYIGR GDMWRLKKSL VSTCAYITQK VEFAGIRAQA G ELWVKNEK ...String:
MGDYKDDDDK GGGGGASTMR TTKVYKLVIH KKGFGGSDDE LVVNPKVFPH IKLGDIVEIA HPNDEYSPLL LQVKSLKEDL QKETISVDQ TVTQVFRLRP YQDVYVNVVD PKDVTLDLVE LTFKDQYIGR GDMWRLKKSL VSTCAYITQK VEFAGIRAQA G ELWVKNEK VMCGYISEDT RVVFRSTSAM VYIFIQMSCE MWDFDIYGDL YFEKAVNGFL ADLFTKWKEK NCSHEVTVVL FS RTFYDAK SVDEFPEINR ASIRQDHKGR FYEDFYKVVV QNERREEWTS LLVTIKKLFI QYPVLVRLEQ AEGFPQGDNS TSA QGNYLE AINLSFNVFD KHYINRNFDR TGQMSVVITP GVGVFEVDRL LMILTKQRMI DNGIGVDLVC MGEQPLHAVP LFKL HNRSA PRDSRLGDDY NIPHWINHSF YTSKSQLFCN SFTPRIKLAG KKPASEKAKN GRDTSLGSPK ESENALPIQV DYDAY DAQV FRLPGPSRAQ CLTTCRSVRE RESHSRKSAS SCDVSSSPSL PSRTLPTEEV RSQASDDSSL GKSANILMIP HPHLHQ YEV SSSLGYTSTR DVLENMMEPP QRDSSAPGRF HVGSAESMLH VRPGGYTPQR ALINPFAPSR MPMKLTSNRR RWMHTFP VG PSGEAIQIHH QTRQNMAELQ GSGQRDPTHS SAELLELAYH EAAGRHSNSR QPGDGMSFLN FSGTEELSVG LLSNSGAG M NPRTQNKDSL EDSVSTSPDP ILTLSAPPVV PGFCCTVGVD WKSLTTPACL PLTTDYFPDR QGLQNDYTEG CYDLLPEAD IDRRDEDGVQ MTAQQVFEEF ICQRLMQGYQ IIVQPKTQKP NPAVPPPLSS SPLYSRGLVS RNRPEEEDQY WLSMGRTFHK VTLKDKMIT VTRYLPKYPY ESAQIHYTYS LCPSHSDSEF VSCWVEFSHE RLEEYKWNYL DQYICSAGSE DFSLIESLKF W RTRFLLLP ACVTATKRIT EGEAHCDIYG DRPRADEDEW QLLDGFVRFV EGLNRIRRRH RSDRMMRKGT AMKGLQMTGP IS THSLEST APPVGKKGTS ALSALLEMEA SQKCLGEQQA AVHGGKSSAQ SAESSSVAMT PTYMDSPRKD GAFFMEFVRS PRT ASSAFY PQVSVDQTAT PMLDGTSLGI CTGQSMDRGN SQTFGNSQNI GEQGYSSTNS SDSSSQQLVA SSLTSSSTLT EILE AMKHP STGVQLLSEQ KGLSPYCFIS AEVVHWLVNH VEGIQTQAMA IDIMQKMLEE QLITHASGEA WRTFIYGFYF YKIVT DKEP DRVAMQQPAT TWHTAGVDDF ASFQRKWFEV AFVAEELVHS EIPAFLLPWL PSRPASYASR HSSFSRSFGG RSQAAA LLA ATVPEQRTVT LDVDVNNRTD RLEWCSCYYH GNFSLNAAFE IKLHWMAVTA AVLFEMVQGW HRKATSCGFL LVPVLEG PF ALPSYLYGDP LRAQLFIPLN ISCLLKEGSE HLFDSFEPET YWDRMHLFQE AIAHRFGFVQ DKYSASAFNF PAENKPQY I HVTGTVFLQL PYSKRKFSGQ QRRRRNSTSS TNQNMFCEER VGYNWAYNTM LTKTWRSSAT GDEKFADRLL KDFTDFCIN RDNRLVTFWT SCLEKMHASA P

UniProtKB: GATOR1 complex protein DEPDC5

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.8 mg/mL
BufferpH: 7.4
GridModel: Quantifoil R1.2/1.3 / Support film - Material: CARBON / Support film - topology: HOLEY
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 278 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS TALOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: COUNTING / Average electron dose: 1.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER / Details: Ab initio
Final reconstructionApplied symmetry - Point group: C2 (2 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 5.0 Å / Resolution method: OTHER / Details: Average nominal resolution of composite / Number images used: 13688
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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Atomic model buiding 1

RefinementProtocol: FLEXIBLE FIT
Output model

PDB-9o5e:
The dimeric KICSTOR-GATOR1 supercomplex

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Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

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