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- EMDB-70095: 80S Ribosome Rat Brain polysome fracAon. Class 2 -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-70095
Title80S Ribosome Rat Brain polysome fracAon. Class 2
Map dataConsensus map
Sample
  • Complex: 80S Ribosome Rat Brain polysome fraction. Class 1
KeywordsRibosome / Rat / Brain / Polysome
Biological speciesRattus norvegicus (Norway rat)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsOrtega J / Sun J
Funding support Canada, 1 items
OrganizationGrant numberCountry
Canadian Institutes of Health Research (CIHR) Canada
CitationJournal: Elife / Year: 2026
Title: FMRP regulates neuronal RNA granules containing stalled ribosomes, not where ribosomes stall
Authors: Li J / Amiri M / Kailasam S / Sun J / Sonenberg N / Ortega J / Sossin WS
History
DepositionApr 8, 2025-
Header (metadata) releaseFeb 18, 2026-
Map releaseFeb 18, 2026-
UpdateFeb 18, 2026-
Current statusFeb 18, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_70095.png

Downloads & links

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Map

FileDownload / File: emd_70095.map.gz / Format: CCP4 / Size: 343 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationConsensus map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.09 Å/pix.
x 448 pix.
= 488.32 Å		1.09 Å/pix.
x 448 pix.
= 488.32 Å		1.09 Å/pix.
x 448 pix.
= 488.32 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.09 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.25
Minimum - Maximum-0.2327766 - 0.98963845
Average (Standard dev.)0.0071191513 (±0.053277355)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions448448448
Spacing448448448
CellA=B=C: 488.32 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: 60S locally refined

Fileemd_70095_additional_1.map
Annotation60S locally refined
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: 40S locally refined

Fileemd_70095_additional_2.map
Annotation40S locally refined
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Composite map

Fileemd_70095_additional_3.map
AnnotationComposite map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map

Fileemd_70095_half_map_1.map
AnnotationHalf map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_70095_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : 80S Ribosome Rat Brain polysome fraction. Class 1

EntireName: 80S Ribosome Rat Brain polysome fraction. Class 1
Components
  • Complex: 80S Ribosome Rat Brain polysome fraction. Class 1

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Supramolecule #1: 80S Ribosome Rat Brain polysome fraction. Class 1

SupramoleculeName: 80S Ribosome Rat Brain polysome fraction. Class 1 / type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Rattus norvegicus (Norway rat)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 141803
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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