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- EMDB-70068: Replisome (map E) -

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Basic information

Entry
Database: EMDB / ID: EMD-70068
TitleReplisome (map E)
Map data
Sample
  • Cell: Human Replisome complex
    • Protein or peptide: x 15 types
    • DNA: x 2 types
  • Ligand: x 3 types
KeywordsDNA replication / CMG / TIMELESS-TIPIN / replisome / AND-1 / CLASPIN / REPLICATION
Function / homology
Function and homology information


cellular response to bleomycin / regulation of nuclear cell cycle DNA replication / DNA secondary structure binding / detection of abiotic stimulus / replication fork protection complex / replication fork arrest / cell cycle phase transition / Switching of origins to a post-replicative state / Unwinding of DNA / DNA strand elongation involved in mitotic DNA replication ...cellular response to bleomycin / regulation of nuclear cell cycle DNA replication / DNA secondary structure binding / detection of abiotic stimulus / replication fork protection complex / replication fork arrest / cell cycle phase transition / Switching of origins to a post-replicative state / Unwinding of DNA / DNA strand elongation involved in mitotic DNA replication / cellular response to hydroxyurea / GINS complex / mitotic DNA replication preinitiation complex assembly / cellular response to cisplatin / nuclear origin of replication recognition complex / anaphase-promoting complex binding / alpha DNA polymerase:primase complex / regulation of phosphorylation / morphogenesis of an epithelium / Regulation of MITF-M-dependent genes involved in DNA replication, damage repair and senescence / CMG complex / DNA replication checkpoint signaling / DNA replication preinitiation complex / mitotic DNA replication checkpoint signaling / MCM complex / mitotic DNA replication initiation / mitotic intra-S DNA damage checkpoint signaling / mitotic DNA replication / Apoptotic cleavage of cellular proteins / double-strand break repair via break-induced replication / DNA strand elongation involved in DNA replication / G1/S-Specific Transcription / mitotic G2 DNA damage checkpoint signaling / positive regulation of double-strand break repair / nuclear replication fork / cochlea development / DNA replication origin binding / Activation of the pre-replicative complex / DNA replication initiation / Activation of ATR in response to replication stress / regulation of DNA-templated DNA replication initiation / DNA damage checkpoint signaling / positive regulation of double-strand break repair via homologous recombination / cellular response to epidermal growth factor stimulus / replication fork processing / circadian rhythm / Assembly of the pre-replicative complex / DNA-templated DNA replication / regulation of circadian rhythm / enzyme activator activity / Orc1 removal from chromatin / mitotic cell cycle / histone binding / single-stranded DNA binding / site of double-strand break / ciliary basal body / Processing of DNA double-strand break ends / DNA helicase / DNA replication / apoptotic process / chromosome, telomeric region / DNA helicase activity / response to xenobiotic stimulus / Ub-specific processing proteases / DNA repair / chromosome / negative regulation of DNA-templated transcription / chromatin binding / positive regulation of cell population proliferation / DNA damage response / chromatin / perinuclear region of cytoplasm / Golgi apparatus / enzyme binding / ATP hydrolysis activity / DNA binding / nucleoplasm / ATP binding / membrane / identical protein binding / nucleus / cytosol / cytoplasm
Similarity search - Function
: / Claspin / : / : / WDHD1 HMG box / WDHD1 first WD40 domain / Claspin / : / TIMELESS, tri-helical helix-turn-helix domain / Chromosome segregation in meiosis protein 3 ...: / Claspin / : / : / WDHD1 HMG box / WDHD1 first WD40 domain / Claspin / : / TIMELESS, tri-helical helix-turn-helix domain / Chromosome segregation in meiosis protein 3 / TIPIN/Csm3/Swi3 / Replication Fork Protection Component Swi3 / Timeless, C-terminal / Timeless-like, PAB domain / : / TOF1-like, helical domain / Timeless, N-terminal / Timeless / Timeless protein / : / DNA polymerase alpha-binding protein Ctf4, C-terminal domain / Minichromosome loss protein Mcl1, middle region / Minichromosome loss protein, Mcl1, middle region / : / DNA replication licensing factor MCM2-like, winged-helix domain / : / PSF2 N-terminal domain / : / : / PSF3 N-terminal domain / PSF1 C-terminal domain / DNA replication complex GINS protein Psf2 / GINS complex, subunit Psf3 / DNA replication complex GINS protein SLD5, C-terminal / GINS complex, subunit Psf3 superfamily / GINS complex protein Sld5, alpha-helical domain / DNA replication complex GINS protein SLD5 C-terminus / GINS complex subunit Sld5 / CDC45 family / GINS subunit, domain A / CDC45 / GINS complex protein helical bundle domain / GINS complex, subunit Psf1 / GINS, helical bundle-like domain superfamily / : / MCM5, C-terminal domain / DNA replication licensing factor MCM7, winged helix / : / DNA replication licensing factor Mcm5 / MCM4, winged helix domain / Mini-chromosome maintenance complex protein 4 / DNA replication licensing factor Mcm3 / : / MCM3-like, winged helix domain / DNA replication licensing factor Mcm7 / DNA replication licensing factor Mcm6 / Mcm6, C-terminal winged-helix domain / MCM6 C-terminal winged-helix domain / DNA replication licensing factor Mcm2 / Mini-chromosome maintenance protein 2 / Mini-chromosome maintenance, conserved site / MCM family signature. / MCM N-terminal domain / MCM N-terminal domain / MCM OB domain / MCM OB domain / Mini-chromosome maintenance protein / MCM, AAA-lid domain / MCM P-loop domain / MCM AAA-lid domain / MCM family C-terminal AAA(+) ATPase domain (MCM-CTD) profile. / minichromosome maintenance proteins / MCM domain / HMG boxes A and B DNA-binding domains profile. / high mobility group / High mobility group box domain / High mobility group box domain superfamily / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Nucleic acid-binding, OB-fold / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Cell division control protein 45 homolog / WD repeat and HMG-box DNA-binding protein 1 / DNA replication licensing factor MCM3 / DNA replication licensing factor MCM4 / DNA replication licensing factor MCM5 / DNA replication licensing factor MCM7 / DNA replication licensing factor MCM2 / DNA replication licensing factor MCM6 / DNA replication complex GINS protein PSF1 / DNA replication complex GINS protein SLD5 ...Cell division control protein 45 homolog / WD repeat and HMG-box DNA-binding protein 1 / DNA replication licensing factor MCM3 / DNA replication licensing factor MCM4 / DNA replication licensing factor MCM5 / DNA replication licensing factor MCM7 / DNA replication licensing factor MCM2 / DNA replication licensing factor MCM6 / DNA replication complex GINS protein PSF1 / DNA replication complex GINS protein SLD5 / DNA replication complex GINS protein PSF3 / TIMELESS-interacting protein / Claspin / Protein timeless homolog / DNA replication complex GINS protein PSF2
Similarity search - Component
Biological speciesHomo sapiens (human) / Homo sapiens environmental sample (environmental samples) / synthetic construct (others)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.1 Å
AuthorsSteinruecke F / Markert J / Farnung L
Funding support United States, 2 items
OrganizationGrant numberCountry
National Science Foundation (NSF, United States)DGE 2140743 United States
National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)DP2-ES036404 United States
CitationJournal: To Be Published
Title: Structural basis of human replisome progression into a nucleosome
Authors: Steinruecke F / Markert J / Farnung L
History
DepositionApr 4, 2025-
Header (metadata) releaseOct 14, 2026-
Map releaseOct 14, 2026-
UpdateOct 14, 2026-
Current statusOct 14, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_70068.png

Downloads & links

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Map

FileDownload / File: emd_70068.map.gz / Format: CCP4 / Size: 476.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.94 Å/pix.
x 500 pix.
= 470. Å		0.94 Å/pix.
x 500 pix.
= 470. Å		0.94 Å/pix.
x 500 pix.
= 470. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.94 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.00859
Minimum - Maximum-0.0018681925 - 1.6922537
Average (Standard dev.)0.0009229034 (±0.020937521)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions500500500
Spacing500500500
CellA=B=C: 470.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Human Replisome complex

EntireName: Human Replisome complex
Components
  • Cell: Human Replisome complex
    • Protein or peptide: DNA replication licensing factor MCM2
    • Protein or peptide: DNA replication licensing factor MCM3
    • Protein or peptide: DNA replication licensing factor MCM4
    • Protein or peptide: DNA replication licensing factor MCM5
    • Protein or peptide: DNA replication licensing factor MCM6
    • Protein or peptide: DNA replication licensing factor MCM7
    • Protein or peptide: Cell division control protein 45 homolog
    • Protein or peptide: DNA replication complex GINS protein PSF1
    • Protein or peptide: DNA replication complex GINS protein PSF2
    • Protein or peptide: DNA replication complex GINS protein PSF3
    • Protein or peptide: DNA replication complex GINS protein SLD5
    • Protein or peptide: WD repeat and HMG-box DNA-binding protein 1
    • Protein or peptide: Protein timeless homolog
    • Protein or peptide: TIMELESS-interacting protein
    • DNA: Leading Strand DNA
    • DNA: Lagging strand DNA
    • Protein or peptide: Claspin
  • Ligand: MAGNESIUM ION
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
  • Ligand: ZINC ION

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Supramolecule #1: Human Replisome complex

SupramoleculeName: Human Replisome complex / type: cell / ID: 1 / Parent: 0 / Macromolecule list: #1-#17
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: DNA replication licensing factor MCM2

MacromoleculeName: DNA replication licensing factor MCM2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 102.034102 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MAESSESFTM ASSPAQRRRG NDPLTSSPGR SSRRTDALTS SPGRDLPPFE DESEGLLGTE GPLEEEEDGE ELIGDGMERD YRAIPELDA YEAEGLALDD EDVEELTASQ REAAERAMRQ RDREAGRGLG RMRRGLLYDS DEEDEERPAR KRRQVERATE D GEEDEEMI ...String:
MAESSESFTM ASSPAQRRRG NDPLTSSPGR SSRRTDALTS SPGRDLPPFE DESEGLLGTE GPLEEEEDGE ELIGDGMERD YRAIPELDA YEAEGLALDD EDVEELTASQ REAAERAMRQ RDREAGRGLG RMRRGLLYDS DEEDEERPAR KRRQVERATE D GEEDEEMI ESIENLEDLK GHSVREWVSM AGPRLEIHHR FKNFLRTHVD SHGHNVFKER ISDMCKENRE SLVVNYEDLA AR EHVLAYF LPEAPAELLQ IFDEAALEVV LAMYPKYDRI TNHIHVRISH LPLVEELRSL RQLHLNQLIR TSGVVTSCTG VLP QLSMVK YNCNKCNFVL GPFCQSQNQE VKPGSCPECQ SAGPFEVNME ETIYQNYQRI RIQESPGKVA AGRLPRSKDA ILLA DLVDS CKPGDEIELT GIYHNNYDGS LNTANGFPVF ATVILANHVA KKDNKVAVGE LTDEDVKMIT SLSKDQQIGE KIFAS IAPS IYGHEDIKRG LALALFGGEP KNPGGKHKVR GDINVLLCGD PGTAKSQFLK YIEKVSSRAI FTTGQGASAV GLTAYV QRH PVSREWTLEA GALVLADRGV CLIDEFDKMN DQDRTSIHEA MEQQSISISK AGIVTSLQAR CTVIAAANPI GGRYDPS LT FSENVDLTEP IISRFDILCV VRDTVDPVQD EMLARFVVGS HVRHHPSNKE EEGLANGSAA EPAMPNTYGV EPLPQEVL K KYIIYAKERV HPKLNQMDQD KVAKMYSDLR KESMATGSIP ITVRHIESMI RMAEAHARIH LRDYVIEDDV NMAIRVMLE SFIDTQKFSV MRSMRKTFAR YLSFRRDNNE LLLFILKQLV AEQVTYQRNR FGAQQDTIEV PEKDLVDKAR QINIHNLSAF YDSELFRMN KFSHDLKRKM ILQQF

UniProtKB: DNA replication licensing factor MCM2

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Macromolecule #2: DNA replication licensing factor MCM3

MacromoleculeName: DNA replication licensing factor MCM3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 91.110852 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQSKVRELI SDNQYRLIVN VNDLRRKNEK RANRLLNNAF EELVAFQRAL KDFVASIDA TYAKQYEEFY VGLEGSFGSK HVSPRTLTSC FLSCVVCVEG IVTKCSLVRP KVVRSVHYCP ATKKTIERRY S DLTTLVAF ...String:
MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQSKVRELI SDNQYRLIVN VNDLRRKNEK RANRLLNNAF EELVAFQRAL KDFVASIDA TYAKQYEEFY VGLEGSFGSK HVSPRTLTSC FLSCVVCVEG IVTKCSLVRP KVVRSVHYCP ATKKTIERRY S DLTTLVAF PSSSVYPTKD EENNPLETEY GLSVYKDHQT ITIQEMPEKA PAGQLPRSVD VILDDDLVDK AKPGDRVQVV GT YRCLPGK KGGYTSGTFR TVLIACNVKQ MSKDAQPSFS AEDIAKIKKF SKTRSKDIFD QLAKSLAPSI HGHDYVKKAI LCL LLGGVE RDLENGSHIR GDINILLIGD PSVAKSQLLR YVLCTAPRAI PTTGRGSSGV GLTAAVTTDQ ETGERRLEAG AMVL ADRGV VCIDEFDKMS DMDRTAIHEV MEQGRVTIAK AGIHARLNAR CSVLAAANPV YGRYDQYKTP MENIGLQDSL LSRFD LLFI MLDQMDPEQD REISDHVLRM HRYRAPGEQD GDAMPLGSAV DILATDDPNF SQEDQQDTQI YEKHDNLLHG TKKKKE KMV SAAFMKKYIH VAKIIKPVLT QESATYIAEE YSRLRSQDSM SSDTARTSPV TARTLETLIR LATAHAKARM SKTVDLQ DA EEAVELVQYA YFKKVLEKEK KRKKRSEDES ETEDEEEKSQ EDQEQKRKRR KTRQPDAKDG DSYDPYDFSD TEEEMPQV H TPKTADSQET KESQKVELSE SRLKAFKVAL LDVFREAHAQ SIGMNRLTES INRDSEEPFS SVEIQAALSK MQDDNQVMV SEGIIFLI

UniProtKB: DNA replication licensing factor MCM3

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Macromolecule #3: DNA replication licensing factor MCM4

MacromoleculeName: DNA replication licensing factor MCM4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens environmental sample (environmental samples)
Molecular weightTheoretical: 96.684852 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSSPASTPSR RGSRRGRATP AQTPRSEDAR SSPSQRRRGE DSTSTGELQP MPTSPGVDLQ SPAAQDVLFS SPPQMHSSAI PLDFDVSSP LTYGTPSSRV EGTPRSGVRG TPVRQRPDLG SAQKGLQVDL QSDGAAAEDI VASEQSLGQK LVIWGTDVNV A ACKENFQR ...String:
MSSPASTPSR RGSRRGRATP AQTPRSEDAR SSPSQRRRGE DSTSTGELQP MPTSPGVDLQ SPAAQDVLFS SPPQMHSSAI PLDFDVSSP LTYGTPSSRV EGTPRSGVRG TPVRQRPDLG SAQKGLQVDL QSDGAAAEDI VASEQSLGQK LVIWGTDVNV A ACKENFQR FLQRFIDPLA KEEENVGIDI TEPLYMQRLG EINVIGEPFL NVNCEHIKSF DKNLYRQLIS YPQEVIPTFD MA VNEIFFD RYPDSILEHQ IQVRPFNALK TKNMRNLNPE DIDQLITISG MVIRTSQLIP EMQEAFFQCQ VCAHTTRVEM DRG RIAEPS VCGRCHTTHS MALIHNRSLF SDKQMIKLQE SPEDMPAGQT PHTVILFAHN DLVDKVQPGD RVNVTGIYRA VPIR VNPRV SNVKSVYKTH IDVIHYRKTD AKRLHGLDEE AEQKLFSEKR VELLKELSRK PDIYERLASA LAPSIYEHED IKKGI LLQL FGGTRKDFSH TGRGKFRAEI NILLCGDPGT SKSQLLQYVY NLVPRGQYTS GKGSSAVGLT AYVMKDPETR QLVLQT GAL VLSDNGICCI DEFDKMNEST RSVLHEVMEQ QTLSIAKAGI ICQLNARTSV LAAANPIESQ WNPKKTTIEN IQLPHTL LS RFDLIFLLLD PQDEAYDRRL AHHLVALYYQ SEEQAEEELL DMAVLKDYIA YAHSTIMPRL SEEASQALIE AYVDMRKI G SSRGMVSAYP RQLESLIRLA EAHAKVRLSN KVEAIDVEEA KRLHREALKQ SATDPRTGIV DISILTTGMS ATSRKRKEE LAEALKKLIL SKGKTPALKY QQLFEDIRGQ SDIAITKDMF EEALRALADD DFLTVTGKTV RLL

UniProtKB: DNA replication licensing factor MCM4

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Macromolecule #4: DNA replication licensing factor MCM5

MacromoleculeName: DNA replication licensing factor MCM5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 82.406633 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSGFDDPGIF YSDSFGGDAQ ADEGQARKSQ LQRRFKEFLR QYRVGTDRTG FTFKYRDELK RHYNLGEYWI EVEMEDLASF DEDLADYLY KQPAEHLQLL EEAAKEVADE VTRPRPSGEE VLQDIQVMLK SDASPSSIRS LKSDMMSHLV KIPGIIIAAS A VRAKATRI ...String:
MSGFDDPGIF YSDSFGGDAQ ADEGQARKSQ LQRRFKEFLR QYRVGTDRTG FTFKYRDELK RHYNLGEYWI EVEMEDLASF DEDLADYLY KQPAEHLQLL EEAAKEVADE VTRPRPSGEE VLQDIQVMLK SDASPSSIRS LKSDMMSHLV KIPGIIIAAS A VRAKATRI SIQCRSCRNT LTNIAMRPGL EGYALPRKCN TDQAGRPKCP LDPYFIMPDK CKCVDFQTLK LQELPDAVPH GE MPRHMQL YCDRYLCDKV VPGNRVTIMG IYSIKKFGLT TSRGRDRVGV GIRSSYIRVL GIQVDTDGSG RSFAGAVSPQ EEE EFRRLA ALPNVYEVIS KSIAPSIFGG TDMKKAIACL LFGGSRKRLP DGLTRRGDIN LLMLGDPGTA KSQLLKFVEK CSPI GVYTS GKGSSAAGLT ASVMRDPSSR NFIMEGGAMV LADGGVVCID EFDKMREDDR VAIHEAMEQQ TISIAKAGIT TTLNS RCSV LAAANSVFGR WDETKGEDNI DFMPTILSRF DMIFIVKDEH NEERDVMLAK HVITLHVSAL TQTQAVEGEI DLAKLK KFI AYCRVKCGPR LSAEAAEKLK NRYIIMRSGA RQHERDSDRR SSIPITVRQL EAIVRIAEAL SKMKLQPFAT EADVEEA LR LFQVSTLDAA LSGTLSGVEG FTSQEDQEML SRIEKQLKRR FAIGSQVSEH SIIKDFTKQK YPEHAIHKVL QLMLRRGE I QHRMQRKVLY RLK

UniProtKB: DNA replication licensing factor MCM5

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Macromolecule #5: DNA replication licensing factor MCM6

MacromoleculeName: DNA replication licensing factor MCM6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 93.010273 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MDLAAAAEPG AGSQHLEVRD EVAEKCQKLF LDFLEEFQSS DGEIKYLQLA EELIRPERNT LVVSFVDLEQ FNQQLSTTIQ EEFYRVYPY LCRALKTFVK DRKEIPLAKD FYVAFQDLPT RHKIRELTSS RIGLLTRISG QVVRTHPVHP ELVSGTFLCL D CQTVIRDV ...String:
MDLAAAAEPG AGSQHLEVRD EVAEKCQKLF LDFLEEFQSS DGEIKYLQLA EELIRPERNT LVVSFVDLEQ FNQQLSTTIQ EEFYRVYPY LCRALKTFVK DRKEIPLAKD FYVAFQDLPT RHKIRELTSS RIGLLTRISG QVVRTHPVHP ELVSGTFLCL D CQTVIRDV EQQFKYTQPN ICRNPVCANR RRFLLDTNKS RFVDFQKVRI QETQAELPRG SIPRSLEVIL RAEAVESAQA GD KCDFTGT LIVVPDVSKL STPGARAETN SRVSGVDGYE TEGIRGLRAL GVRDLSYRLV FLACCVAPTN PRFGGKELRD EEQ TAESIK NQMTVKEWEK VFEMSQDKNL YHNLCTSLFP TIHGNDEVKR GVLLMLFGGV PKTTGEGTSL RGDINVCIVG DPST AKSQF LKHVEEFSPR AVYTSGKASS AAGLTAAVVR DEESHEFVIE AGALMLADNG VCCIDEFDKM DVRDQVAIHE AMEQQ TISI TKAGVKATLN ARTSILAAAN PISGHYDRSK SLKQNINLSA PIMSRFDLFF ILVDECNEVT DYAIARRIVD LHSRIE ESI DRVYSLDDIR RYLLFARQFK PKISKESEDF IVEQYKHLRQ RDGSGVTKSS WRITVRQLES MIRLSEAMAR MHCCDEV QP KHVKEAFRLL NKSIIRVETP DVNLDQEEEI QMEVDEGAGG INGHADSPAP VNGINGYNED INQESAPKAS LRLGFSEY C RISNLIVLHL RKVEEEEDES ALKRSELVNW YLKEIESEID SEEELINKKR IIEKVIHRLT HYDHVLIELT QAGLKGSTE GSESYEEDPY LVVNPNYLLE D

UniProtKB: DNA replication licensing factor MCM6

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Macromolecule #6: DNA replication licensing factor MCM7

MacromoleculeName: DNA replication licensing factor MCM7 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 81.411875 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MALKDYALEK EKVKKFLQEF YQDDELGKKQ FKYGNQLVRL AHREQVALYV DLDDVAEDDP ELVDSICENA RRYAKLFADA VQELLPQYK EREVVNKDVL DVYIEHRLMM EQRSRDPGMV RSPQNQYPAE LMRRFELYFQ GPSSNKPRVI REVRADSVGK L VTVRGIVT ...String:
MALKDYALEK EKVKKFLQEF YQDDELGKKQ FKYGNQLVRL AHREQVALYV DLDDVAEDDP ELVDSICENA RRYAKLFADA VQELLPQYK EREVVNKDVL DVYIEHRLMM EQRSRDPGMV RSPQNQYPAE LMRRFELYFQ GPSSNKPRVI REVRADSVGK L VTVRGIVT RVSEVKPKMV VATYTCDQCG AETYQPIQSP TFMPLIMCPS QECQTNRSGG RLYLQTRGSR FIKFQEMKMQ EH SDQVPVG NIPRSITVLV EGENTRIAQP GDHVSVTGIF LPILRTGFRQ VVQGLLSETY LEAHRIVKMN KSEDDESGAG ELT REELRQ IAEEDFYEKL AASIAPEIYG HEDVKKALLL LLVGGVDQSP RGMKIRGNIN ICLMGDPGVA KSQLLSYIDR LAPR SQYTT GRGSSGVGLT AAVLRDSVSG ELTLEGGALV LADQGVCCID EFDKMAEADR TAIHEVMEQQ TISIAKAGIL TTLNA RCSI LAAANPAYGR YNPRRSLEQN IQLPAALLSR FDLLWLIQDR PDRDNDLRLA QHITYVHQHS RQPPSQFEPL DMKLMR RYI AMCREKQPMV PESLADYITA AYVEMRREAW ASKDATYTSA RTLLAILRLS TALARLRMVD VVEKEDVNEA IRLMEMS KD SLLGDKGQTA RTQRPADVIF ATVRELVSGG RSVRFSEAEQ RCVSRGFTPA QFQAALDEYE ELNVWQVNAS RTRITFV

UniProtKB: DNA replication licensing factor MCM7

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Macromolecule #7: Cell division control protein 45 homolog

MacromoleculeName: Cell division control protein 45 homolog / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 66.016891 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MFVSDFRKEF YEVVQSQRVL LFVASDVDAL CACKILQALF QCDHVQYTLV PVSGWQELET AFLEHKEQFH YFILINCGAN VDLLDILQP DEDTIFFVCD THRPVNVVNV YNDTQIKLLI KQDDDLEVPA YEDIFRDEEE DEEHSGNDSD GSEPSEKRTR L DYKDDDIV ...String:
MFVSDFRKEF YEVVQSQRVL LFVASDVDAL CACKILQALF QCDHVQYTLV PVSGWQELET AFLEHKEQFH YFILINCGAN VDLLDILQP DEDTIFFVCD THRPVNVVNV YNDTQIKLLI KQDDDLEVPA YEDIFRDEEE DEEHSGNDSD GSEPSEKRTR L DYKDDDIV EQTMRRRQRR EWEARRRDIL FDYEQYEYHG TSSAMVMFEL AWMLSKDLND MLWWAIVGLT DQWVQDKITQ MK YVTDVGV LQRHVSRHNH RNEDEENTLS VDCTRISFEY DLRLVLYQHW SLHDSLCNTS YTAARFKLWS VHGQKRLQEF LAD MGLPLK QVKQKFQAMD ISLKENLREM IEESANKFGM KDMRVQTFSI HFGFKHKFLA SDVVFATMSL MESPEKDGSG TDHF IQALD SLSRSNLDKL YHGLELAKKQ LRATQQTIAS CLCTNLVISQ GPFLYCSLME GTPDVMLFSR PASLSLLSKH LLKSF VCST KNRRCKLLPL VMAAPLSMEH GTVTVVGIPP ETDSSDRKNF FGRAFEKAAE STSSRMLHNH FDLSVIELKA EDRSKF LDA LISLLS

UniProtKB: Cell division control protein 45 homolog

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Macromolecule #8: DNA replication complex GINS protein PSF1

MacromoleculeName: DNA replication complex GINS protein PSF1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.022469 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MFCEKAMELI RELHRAPEGQ LPAFNEDGLR QVLEEMKALY EQNQSDVNEA KSGGRSDLIP TIKFRHCSLL RNRRCTVAYL YDRLLRIRA LRWEYGSVLP NALRFHMAAE EMEWFNNYKR SLATYMRSLG GDEGLDITQD MKPPKSLYIE VRCLKDYGEF E VDDGTSVL ...String:
MFCEKAMELI RELHRAPEGQ LPAFNEDGLR QVLEEMKALY EQNQSDVNEA KSGGRSDLIP TIKFRHCSLL RNRRCTVAYL YDRLLRIRA LRWEYGSVLP NALRFHMAAE EMEWFNNYKR SLATYMRSLG GDEGLDITQD MKPPKSLYIE VRCLKDYGEF E VDDGTSVL LKKNSQHFLP RWKCEQLIRQ GVLEHILS

UniProtKB: DNA replication complex GINS protein PSF1

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Macromolecule #9: DNA replication complex GINS protein PSF2

MacromoleculeName: DNA replication complex GINS protein PSF2 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 21.453713 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString:
MDAAEVEFLA EKELVTIIPN FSLDKIYLIG GDLGPFNPGL PVEVPLWLAI NLKQRQKCRL LPPEWMDVEK LEKMRDHERK EETFTPMPS PYYMELTKLL LNHASDNIPK ADEIRTLVKD MWDTRIAKLR VSADSFVRQQ EAHAKLDNLT LMEINTSGTF L TQALNHMY KLRTNLQPLE STQSQDF

UniProtKB: DNA replication complex GINS protein PSF2

+
Macromolecule #10: DNA replication complex GINS protein PSF3

MacromoleculeName: DNA replication complex GINS protein PSF3 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.562611 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSEAYFRVES GALGPEENFL SLDDILMSHE KLPVRTETAM PRLGAFFLER SAGAETDNAV PQGSKLELPL WLAKGLFDNK RRILSVELP KIYQEGWRTV FSADPNVVDL HKMGPHFYGF GSQLLHFDSP ENADISQSLL QTFIGRFRRI MDSSQNAYNE D TSALVARL ...String:
MSEAYFRVES GALGPEENFL SLDDILMSHE KLPVRTETAM PRLGAFFLER SAGAETDNAV PQGSKLELPL WLAKGLFDNK RRILSVELP KIYQEGWRTV FSADPNVVDL HKMGPHFYGF GSQLLHFDSP ENADISQSLL QTFIGRFRRI MDSSQNAYNE D TSALVARL DEMERGLFQT GQKGLNDFQC WEKGQASQIT ASNLVQNYKK RKFTDMED

UniProtKB: DNA replication complex GINS protein PSF3

+
Macromolecule #11: DNA replication complex GINS protein SLD5

MacromoleculeName: DNA replication complex GINS protein SLD5 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 30.272389 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSAWSHPQFE KGGGSGGGSG GSAWSHPQFE KSGLEVLFQG PMTEEVDFLG QDSDGGSEEV VLTPAELIER LEQAWMNEKF APELLESKP EIVECVMEQL EHMEENLRRA KREDLKVSIH QMEMERIRYV LSSYLRCRLM KIEKFFPHVL EKEKTRPEGE P SSLSPEEL ...String:
MSAWSHPQFE KGGGSGGGSG GSAWSHPQFE KSGLEVLFQG PMTEEVDFLG QDSDGGSEEV VLTPAELIER LEQAWMNEKF APELLESKP EIVECVMEQL EHMEENLRRA KREDLKVSIH QMEMERIRYV LSSYLRCRLM KIEKFFPHVL EKEKTRPEGE P SSLSPEEL AFAREFMANT ESYLKNVALK HMPPNLQKVD LFRAVPKPDL DSYVFLRVRE RQENILVEPD TDEQRDYVID LE KGSQHLI RYKTIAPLVA SGAVQLI

UniProtKB: DNA replication complex GINS protein SLD5

+
Macromolecule #12: WD repeat and HMG-box DNA-binding protein 1

MacromoleculeName: WD repeat and HMG-box DNA-binding protein 1 / type: protein_or_peptide / ID: 12 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 126.396453 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNAMPATRKP MRYGHTEGHT EVCFDDSGSF IVTCGSDGDV RIWEDLDDDD PKFINVGEKA YSCALKSGKL VTAVSNNTIQ VHTFPEGVP DGILTRFTTN ANHVVFNGDG TKIAAGSSDF LVKIVDVMDS SQQKTFRGHD APVLSLSFDP KDIFLASASC D GSVRVWQI ...String:
SNAMPATRKP MRYGHTEGHT EVCFDDSGSF IVTCGSDGDV RIWEDLDDDD PKFINVGEKA YSCALKSGKL VTAVSNNTIQ VHTFPEGVP DGILTRFTTN ANHVVFNGDG TKIAAGSSDF LVKIVDVMDS SQQKTFRGHD APVLSLSFDP KDIFLASASC D GSVRVWQI SDQTCAISWP LLQKCNDVIN AKSICRLAWQ PKSGKLLAIP VEKSVKLYRR ESWSHQFDLS DNFISQTLNI VT WSPCGQY LAAGSINGLI IVWNVETKDC MERVKHEKGY AICGLAWHPT CGRISYTDAE GNLGLLENVC DPSGKTSSSK VSS RVEKDY NDLFDGDDMS NAGDFLNDNA VEIPSFSKGI INDDEDDEDL MMASGRPRQR SHILEDDENS VDISMLKTGS SLLK EEEED GQEGSIHNLP LVTSQRPFYD GPMPTPRQKP FQSGSTPLHL THRFMVWNSI GIIRCYNDEQ DNAIDVEFHD TSIHH ATHL SNTLNYTIAD LSHEAILLAC ESTDELASKL HCLHFSSWDS SKEWIIDLPQ NEDIEAICLG QGWAAAATSA LLLRLF TIG GVQKEVFSLA GPVVSMAGHG EQLFIVYHRG TGFDGDQCLG VQLLELGKKK KQILHGDPLP LTRKSYLAWI GFSAEGT PC YVDSEGIVRM LNRGLGNTWT PICNTREHCK GKSDHYWVVG IHENPQQLRC IPCKGSRFPP TLPRPAVAIL SFKLPYCQ I ATEKGQMEEQ FWRSVIFHNH LDYLAKNGYE YEESTKNQAT KEQQELLMKM LALSCKLERE FRCVELADLM TQNAVNLAI KYASRSRKLI LAQKLSELAV EKAAELTATQ VEEEEEEEDF RKKLNAGYSN TATEWSQPRF RNQVEEDAED SGEADDEEKP EIHKPGQNS FSKSTNSSDV SAKSGAVTFS SQGRVNPFKV SASSKEPAMS MNSARSTNIL DNMGKSSKKS TALSRTTNNE K SPIIKPLI PKPKPKQASA ASYFQKRNSQ TNKTEEVKEE NLKNVLSETP AICPPQNTEN QRPKTGFQMW LEENRSNILS DN PDFSDEA DIIKEGMIRF RVLSTEERKV WANKAKGETA SEGTEAKKRK RVVDESDETE NQEEKAKENL NLSKKQKPLD FST NQKLSA FAFKQE

UniProtKB: WD repeat and HMG-box DNA-binding protein 1

+
Macromolecule #13: Protein timeless homolog

MacromoleculeName: Protein timeless homolog / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 139.118234 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: SNAMDLHMMN CELLATCSAL GYLEGDTYHK EPDCLESVKD LIRYLRHEDE TRDVRQQLGA AQILQSDLLP ILTQHHQDKP LFDAVIRLM VNLTQPALLC FGNLPKEPSF RHHFLQVLTY LQAYKEAFAS EKAFGVLSET LYELLQLGWE ERQEEDNLLI E RILLLVRN ...String:
SNAMDLHMMN CELLATCSAL GYLEGDTYHK EPDCLESVKD LIRYLRHEDE TRDVRQQLGA AQILQSDLLP ILTQHHQDKP LFDAVIRLM VNLTQPALLC FGNLPKEPSF RHHFLQVLTY LQAYKEAFAS EKAFGVLSET LYELLQLGWE ERQEEDNLLI E RILLLVRN ILHVPADLDQ EKKIDDDASA HDQLLWAIHL SGLDDLLLFL ASSSAEEQWS LHVLEIVSLM FRDQNPEQLA GV GQGRLAQ ERSADFAELE VLRQREMAEK KTRALQRGNR HSRFGGSYIV QGLKSIGERD LIFHKGLHNL RNYSSDLGKQ PKK VPKRRQ AARELSIQRR SALNVRLFLR DFCSEFLENC YNRLMGSVKD HLLREKAQQH DETYYMWALA FFMAFNRAAS FRPG LVSET LSVRTFHFIE QNLTNYYEMM LTDRKEAASW ARRMHLALKA YQELLATVNE MDISPDEAVR ESSRIIKNNI FYVME YREL FLALFRKFDE RCQPRSFLRD LVETTHLFLK MLERFCRSRG NLVVQNKQKK RRKKKKKVLD QAIVSGNVPS SPEEVE AVW PALAEQLQCC AQNSELSMDS VVPFDAASEV PVEEQRAEAM VRIQDCLLAG QAPQALTLLR SAREVWPEGD VFGSQDI SP EEEIQLLKQI LSAPLPRQQG PEERGAEEEE EEEEEEEEEL QVVQVSEKEF NFLDYLKRFA CSTVVRAYVL LLRSYQQN S AHTNHCIVKM LHRLAHDLKM EALLFQLSVF CLFNRLLSDP AAGAYKELVT FAKYILGKFF ALAAVNQKAF VELLFWKNT AVVREMTEGY GSLDDRSSSR RAPTWSPEEE AHLRELYLAN KDVEGQDVVE AILAHLNTVP RTRKQIIHHL VQMGLADSVK DFQRKGTHI VLWTGDQELE LQRLFEEFRD SDDVLGHIMK NITAKRSRAR IVDKLLALGL VAERRELYKK RQKKLASSIL P NGAESLKD FCQEDLEEEE NLPEEDSEEE EEGGSEAEQV QGSLVLSNEN LGQSLHQEGF SIPLLWLQNC LIRAADDREE DG CSQAVPL VPLTEENEEA MENEQFQQLL RKLGVRPPAS GQETFWRIPA KLSPTQLRRA AASLSQPEEE QKLQPELQPK VPG EQGSDE EHCKEHRAQA LRALLLAHKK KAGLASPEEE DAVGKEPLKA APKKRQLLDS DEEQEEDEGR NRAPELGAPG IQKK KRYQI EDDEDD

UniProtKB: Protein timeless homolog

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Macromolecule #14: TIMELESS-interacting protein

MacromoleculeName: TIMELESS-interacting protein / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 34.600223 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MLEPQENGVI DLPDYEHVED ETFPPFPPPA SPERQDGEGT EPDEESGNGA PVRVPPKRTV KRNIPKLDAQ RLISERGLPA LRHVFDKAK FKGKGHEAED LKMLIRHMEH WAHRLFPKLQ FEDFIDRVEY LGSKKEVQTC LKRIRLDLPI LHEDFVSNND E VAENNEHD ...String:
MLEPQENGVI DLPDYEHVED ETFPPFPPPA SPERQDGEGT EPDEESGNGA PVRVPPKRTV KRNIPKLDAQ RLISERGLPA LRHVFDKAK FKGKGHEAED LKMLIRHMEH WAHRLFPKLQ FEDFIDRVEY LGSKKEVQTC LKRIRLDLPI LHEDFVSNND E VAENNEHD VTSTELDPFL TNLSESEMFA SELSRSLTEE QQQRIERNKQ LALERRQAKL LSNSQTLGND MLMNTPRAHT VE EVNTDED QKEESNGLNE DILDNPCNDA IANTLNEEET LLDQSFKNVQ QQLDATSRNI TEAR

UniProtKB: TIMELESS-interacting protein

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Macromolecule #17: Claspin

MacromoleculeName: Claspin / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 152.104594 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MTGEVGSEVH LEINDPNVIS QEEADSPSDS GQGSYETIGP LSEGDSDEEI FVSKKLKNRK VLQDSDSETE DTNASPEKTT YDSAEEENK ENLYAGKNTK IKRIYKTVAD SDESYMEKSL YQENLEAQVK PCLELSLQSG NSTDFTTDRK SSKKHIHDKE G TAGKAKVK ...String:
MTGEVGSEVH LEINDPNVIS QEEADSPSDS GQGSYETIGP LSEGDSDEEI FVSKKLKNRK VLQDSDSETE DTNASPEKTT YDSAEEENK ENLYAGKNTK IKRIYKTVAD SDESYMEKSL YQENLEAQVK PCLELSLQSG NSTDFTTDRK SSKKHIHDKE G TAGKAKVK SKRRLEKEER KMEKIRQLKK KETKNQEDDV EQPFNDSGCL LVDKDLFETG LEDENNSPLE DEESLESIRA AV KNKVKKH KKKEPSLESG VHSFEEGSEL SKGTTRKERK AARLSKEALK QLHSETQRLI RESALNLPYH MPENKTIHDF FKR KPRPTC HGNAMALLKS SKYQSSHHKE IIDTANTTEM NSDHHSKGSE QTTGAENEVE TNALPVVSKE TQIITGSDES CRKD LVKNE ELEIQEKQKQ SDIRPSPGDS SVLQQESNFL GNNHSEECQV GGLVAFEPHA LEGEGPQNPE ETDEKVEEPE QQNKS SAVG PPEKVRRFTL DRLKQLGVDV SIKPRLGADE DSFVILEPET NRELEALKQR FWKHANPAAK PRAGQTVNVN VIVKDM GTD GKEELKADVV PVTLAPKKLD GASHTKPGEK LQVLKAKLQE AMKLRRFEER QKRQALFKLD NEDGFEEEEE EEEEMTD ES EEDGEEKVEK EEKEEELEEE EEKEEEEEEE GNQETAEFLL SSEEIETKDE KEMDKENNDG SSEIGKAVGF LSVPKSLS S DSTLLLFKDS SSKMGYFPTE EKSETDENSG KQPSKLDEDD SCSLLTKESS HNSSFELIGS TIPSYQPCNR QTGRGTSFF PTAGGFRSPS PGLFRASLVS SASKSSGKLS EPSLPIEDSQ DLYNASPEPK TLFLGAGDFQ FCLEDDTQSQ LLDADGFLNV RNHRNQYQA LKPRLPLASM DENAMDANMD ELLDLCTGKF TSQAEKHLPR KSDKKENMEE LLNLCSGKFT SQDASTPASS E LNKQEKES SMGDPMEEAL ALCSGSFPTD KEEEDEEEEF GDFRLVSNDN EFDSDEDEHS DSGNDLALED HEDDDEEELL KR SEKLKRQ MRLRKYLEDE AEVSGSDVGS EDEYDGEEID EYEEDVIDEV LPSDEELQSQ IKKIHMKTML DDDKRQLRLY QER YLADGD LHSDGPGRMR KFRWKNIDDA SQMDLFHRDS DDDQTEEQLD ESEARWRKER IEREQWLRDM AQQGKITAEE EEEI GEDSQ FMILAKKVTA KALQKNASRP MVIQESKSLL RNPFEAIRPG SAQQVKTGSL LNQPKAVLQK LAALSDHNPS APRNS RNFV FHTLSPVKAE AAKESSKSQV KKRGPSFMTS PSPKHLKTDD STSGLTRSIF KYLESENLYF Q

UniProtKB: Claspin

+
Macromolecule #15: Leading Strand DNA

MacromoleculeName: Leading Strand DNA / type: dna / ID: 15 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 61.523184 KDa
SequenceString: (DA)(DT)(DC)(DA)(DG)(DA)(DA)(DT)(DC)(DC) (DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG) (DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA) (DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG) (DA) (DC)(DA)(DG)(DC)(DT)(DC) ...String:
(DA)(DT)(DC)(DA)(DG)(DA)(DA)(DT)(DC)(DC) (DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG) (DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA) (DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG) (DA) (DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA) (DG)(DC)(DA)(DC)(DC)(DG)(DC)(DT)(DT)(DA) (DA)(DA) (DC)(DG)(DC)(DA)(DC)(DG)(DT) (DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC) (DC)(DC)(DC) (DC)(DG)(DC)(DG)(DT)(DT) (DT)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA) (DA)(DG)(DG)(DG) (DG)(DA)(DT)(DT)(DA) (DC)(DT)(DC)(DC)(DC)(DT)(DA)(DG)(DT)(DC) (DT)(DC)(DC)(DA)(DG) (DG)(DC)(DA)(DC) (DG)(DT)(DG)(DT)(DC)(DA)(DG)(DA)(DT)(DA) (DT)(DA)(DT)(DA)(DC)(DA) (DT)(DC)(DG) (DA)(DA)(DA)(DT)(DT)(DA)(DG)(DA)(DG)(DA) (DA)(DT)(DT)(DG)(DG)(DA)(DG) (DA)(DG) (DT)(DG)(DT)(DG)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)

+
Macromolecule #16: Lagging strand DNA

MacromoleculeName: Lagging strand DNA / type: dna / ID: 16 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 56.347902 KDa
SequenceString: (DG)(DG)(DC)(DA)(DG)(DG)(DC)(DA)(DG)(DG) (DC)(DA)(DG)(DG)(DC)(DA)(DC)(DA)(DC)(DA) (DC)(DT)(DC)(DT)(DC)(DC)(DA)(DA)(DT) (DT)(DC)(DT)(DC)(DT)(DA)(DA)(DT)(DT)(DT) (DC) (DG)(DA)(DT)(DG)(DT)(DA) ...String:
(DG)(DG)(DC)(DA)(DG)(DG)(DC)(DA)(DG)(DG) (DC)(DA)(DG)(DG)(DC)(DA)(DC)(DA)(DC)(DA) (DC)(DT)(DC)(DT)(DC)(DC)(DA)(DA)(DT) (DT)(DC)(DT)(DC)(DT)(DA)(DA)(DT)(DT)(DT) (DC) (DG)(DA)(DT)(DG)(DT)(DA)(DT)(DA) (DT)(DA)(DT)(DC)(DT)(DG)(DA)(DC)(DA)(DC) (DG)(DT) (DG)(DC)(DC)(DT)(DG)(DG)(DA) (DG)(DA)(DC)(DT)(DA)(DG)(DG)(DG)(DA)(DG) (DT)(DA)(DA) (DT)(DC)(DC)(DC)(DC)(DT) (DT)(DG)(DG)(DC)(DG)(DG)(DT)(DT)(DA)(DA) (DA)(DA)(DC)(DG) (DC)(DG)(DG)(DG)(DG) (DG)(DA)(DC)(DA)(DG)(DC)(DG)(DC)(DG)(DT) (DA)(DC)(DG)(DT)(DG) (DC)(DG)(DT)(DT) (DT)(DA)(DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC) (DT)(DA)(DG)(DA)(DG)(DC) (DT)(DG)(DT) (DC)(DT)(DA)(DC)(DG)(DA)(DC)(DC)(DA)(DA) (DT)(DT)(DG)(DA)(DG)(DC)(DG) (DG)(DC) (DC)(DT)(DC)(DG)(DG)(DC)(DA)(DC)(DC)(DG) (DG)(DG)(DA)(DT)(DT)(DC)(DT)(DG) (DA) (DT)

+
Macromolecule #18: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 18 / Number of copies: 4 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #19: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / type: ligand / ID: 19 / Number of copies: 4 / Formula: ANP
Molecular weightTheoretical: 506.196 Da
Chemical component information

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

+
Macromolecule #20: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 20 / Number of copies: 4 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statecell

-
Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.1 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 2128300
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION

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