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- EMDB-7003: Eilat virus/Venezuelan equine encephalitis virus chimeric vaccine... -

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Basic information

Database: EMDB / ID: 7003
TitleEilat virus/Venezuelan equine encephalitis virus chimeric vaccine candidate
SampleEilat virus
SourceEilat virus / virus
Map dataCombined, filtered, unmasked map of Eilat virus/VEEV chimera
Methodsingle particle (icosahedral) reconstruction, at 8.4 Å resolution
AuthorsKaelber JT / Erasmus JH / Weaver SC / Nasar F / Chiu W
CitationJ. Virol., 2018, 92

J. Virol., 2018, 92 Yorodumi Papers
Novel Insect-Specific Eilat Virus-Based Chimeric Vaccine Candidates Provide Durable, Mono- and Multivalent, Single-Dose Protection against Lethal Alphavirus Challenge.
Jesse H Erasmus / Robert L Seymour / Jason T Kaelber / Dal Y Kim / Grace Leal / Michael B Sherman / Ilya Frolov / Wah Chiu / Scott C Weaver / Farooq Nasar

DateDeposition: Aug 31, 2017 / Header (metadata) release: Sep 13, 2017 / Map release: Nov 22, 2017 / Last update: Feb 14, 2018

Structure visualization

  • Surface view with section colored by density value
  • Surface level: 0.175
  • Imaged by UCSF CHIMERA
  • Download
  • Surface view colored by radius
  • Surface level: 0.175
  • Imaged by UCSF CHIMERA
  • Download
3D viewer
Supplemental images

Downloads & links


Fileemd_7003.map.gz (map file in CCP4 format, 226493 KB)
Projections & slices

Image control

AxesZ (Sec.)Y (Row.)X (Col.)
384 pix
2.33 Å/pix.
= 893.184 Å
384 pix
2.33 Å/pix.
= 893.184 Å
384 pix
2.33 Å/pix.
= 893.184 Å



Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Voxel sizeX=Y=Z: 2.326 Å
Contour Level:0.175 (by author), 0.175 (movie #1):
Minimum - Maximum-0.48278403 - 1.2507095
Average (Standard dev.)0.0031908017 (0.066673)


Space Group Number1
Map Geometry
Axis orderXYZ
CellA=B=C: 893.18396 Å
α=β=γ: 90 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.3262.3262.326
M x/y/z384384384
origin x/y/z0.0000.0000.000
length x/y/z893.184893.184893.184
start NX/NY/NZ
MAP C/R/S123
start NC/NR/NS-192-192-192
D min/max/mean-0.4831.2510.003

Supplemental data

Sample components

Entire Eilat virus

EntireName: Eilat virus / Number of components: 1
MassTheoretical: 42 MDa

Component #1: virus, Eilat virus

VirusName: Eilat virus / Class: VIRION / Empty: No / Enveloped: Yes / Isolate: OTHER
MassTheoretical: 42 MDa
SpeciesSpecies: Eilat virus / virus
Source (engineered)Expression System: Aedes albopictus / arthropod / image: Aedes aegypti
Cell of expression system: C7/10
Source (natural)Host Species: Aedes / arthropod / image: Aedes aegypti

Experimental details

Sample preparation

Specimen stateparticle
Sample solutionpH: 7.4
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 %

Electron microscopy imaging

ImagingMicroscope: JEOL 3200FSC
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 25 e/Å2 / Illumination mode: FLOOD BEAM
LensMagnification: 25000 X (nominal) / Cs: 4.7 mm / Imaging mode: BRIGHT FIELD / Energy filter: Omega
Specimen HolderModel: JEOL 3200FSC CRYOHOLDER / Temperature: K ( 86.7 - K)
CameraDetector: OTHER

Image acquisition

Image acquisitionNumber of digital images: 100

Image processing

ProcessingMethod: single particle (icosahedral) reconstruction / Applied symmetry: I (icosahedral) / Number of projections: 4760
3D reconstructionAlgorithm: FOURIER SPACE / Software: MPSA / Resolution: 8.4 Å / Resolution method: FSC 0.143 CUT-OFF
FSC plot (resolution assessment)

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