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- EMDB-69581: The high-order structure of CPL filament-dimer2 map -

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Basic information

Entry
Database: EMDB / ID: EMD-69581
TitleThe high-order structure of CPL filament-dimer2 map
Map data
Sample
  • Complex: oocytes cytoplasmic lattices
Keywordsoocytes cytoplasmic lattices / CELL CYCLE
Biological speciesMus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 16.3 Å
AuthorsLiu Q / Gui M
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: Vita / Year: 2026
Title: Hierarchical assembly of native cytoplasmic lattices revealed by cryo-EM
Authors: Zhang YL / Liu Y / Liu Q / Zhang Q / Jin J / Liu S / Yang S / Liu Y / Peng YJ / Lai M / Sun Y / Zhang YY / Mei Y / Tong X / Zhang S / Gui M
History
DepositionMar 6, 2026-
Header (metadata) releaseJul 22, 2026-
Map releaseJul 22, 2026-
UpdateJul 22, 2026-
Current statusJul 22, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_69581.png

Downloads & links

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Map

FileReleased
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
3.72 Å/pix.
x 320 pix.
= 1190.4 Å		3.72 Å/pix.
x 320 pix.
= 1190.4 Å		3.72 Å/pix.
x 320 pix.
= 1190.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 3.72 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.02
Minimum - Maximum-0.026355453 - 0.07465424
Average (Standard dev.)0.00047653387 (±0.0036851172)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 1190.4 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_69581_additional_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_69581_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_69581_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : oocytes cytoplasmic lattices

EntireName: oocytes cytoplasmic lattices
Components
  • Complex: oocytes cytoplasmic lattices

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Supramolecule #1: oocytes cytoplasmic lattices

SupramoleculeName: oocytes cytoplasmic lattices / type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Mus musculus (house mouse)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

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Sample preparation

BufferpH: 7.2
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: EMDB MAP
EMDB ID:

16458

Final reconstructionResolution.type: BY AUTHOR / Resolution: 16.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 5.0) / Number images used: 40130
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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